Single Bead Affinity Detection (SINBAD) for the analysis of protein interactions

PLoS ONE

Volumn 3, Issue 4, 2008, Pages

  Schulte, Roberta ^a   Talamas, Jessica ^a   Doucet, Christine ^a   Hetzer, Martin W ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KARYOPHERIN ALPHA; KARYOPHERIN BETA; QUANTUM DOT; DNA; PROTEIN;

AFFINITY CHROMATOGRAPHY; ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SINGLE BEAD AFFINITY DETECTION; ANIMAL; HUMAN; METABOLISM; METHODOLOGY; XENOPUS;

ANIMALS; CHROMATOGRAPHY, AFFINITY; DNA; HUMANS; PROTEIN INTERACTION MAPPING; PROTEINS; XENOPUS;

EID: 44349090258     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0002061     Document Type: Article

References (20)

1. Burbulis I, Yamaguchi K, Yu R, Resnekov O, Brent R (2007) Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera. Nat Methods 4: 1011-1013.
2. Georganopoulou DG, Chang L, Nam JM, Thaxton CS, Mufson EJ, et al. (2005) Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease. Proc Natl Acad Sci U S A 102: 2273-2276.
3. Oh BK, Nam JM, Lee SW, Mirkin CA (2006) A fluorophore-based bio-barcode amplification assay for proteins. Small 2: 103-108.
4. Patolsky F, Zheng G, Lieber CM (2006) Nanowire-based biosensors. Anal Cbem 78: 4260-4269.
5. Gorlich D, Kutay U (1999) Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol 15: 607-660.
6. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, et al. (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307: 538-544.
7. Gorlich D (1997) Nuclear protein import. Curr Opin Cell Biol 9: 412-419.
8. Bischoff FR, Gorlich D (1997) RanBPI is crucial for the release of RanGTP from importin beta-related nuclear transport factors. FEBS Lett 419: 249-254.
9. Gorlich D, Pante N, Kutay U, Aebi U, Bischoff FR (1996) Identification of different roles for RanGDP and RanGTP in nuclear protein import. Embo J 15: 5584-5594.
10. Hetzer M, Walther TC, Mattaj IW (2005) Pushing the Envelope: Structure, Function, and Dynamics of the Nuclear Periphery. Annu Rev Cell Dev Biol.
11. Wente SR (2000) Gatekeepers of the nucleus. Science 288: 1374-1377.
12. Weis K (2003) Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell 112: 441-451.
13. Walther TC, Alves A, Pickersgill H, Loiodice I, Hetzer M, et al. (2003) The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell 113: 195-206.
14. Lutzmann M, Kunze R, Buerer A, Aebi U, Hurt E (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. Embo J 21: 387-397.
15. Zhang CY, Johnson LW (2006) Homogenous rapid detection of nucleic acids using two-color quantum dots. Analyst 131: 484-488.
16. Stansel RM, de Lange T, Griffith JD (2001) T-loop assembly in vitro involves binding of TRF2 near the 3′ telomeric overhang. Embo J 20: 5532-5540.
17. Patel SS, Rexach MF (2008) Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics 7: 121-131.
18. D'Angelo MA, Anderson DJ, Richard E, Hetzer MW (2006) Nuclear pores form de novo from both sides of the nuclear envelope. Science 312: 440-443.
19. Anderson DJ, Hetzer MW (2007) Nuclear Envelope Formation by Chromatin-mediated Reorganization of the Endoplasmic Reticulum. Nature Cell Biology 9: 1160-1166.
20. Franz C, Walczak R, Yavuz S, Santarella R, Gentzel M, et al. (2007) MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly. EMBO Rep 8: 165-172.