Imaging Beads-Retained Prey Assay for Rapid and Quantitative Protein-Protein Interaction

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  Zhou, Yan ^a   Hong, Wanjin ^b   Lu, Lei ^a  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTOR PROTEIN; BINDING PROTEIN; FURIN; GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN ARL1; PROTEIN GRIP; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; IMAGING BEADS RETAINED PREY ASSAY; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; VALIDATION STUDY;

ANIMALS; CLONING, MOLECULAR; CYTOSOL; FURIN; GLUTATHIONE; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; IMMOBILIZED PROTEINS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; OPEN READING FRAMES; PROTEIN INTERACTION MAPPING; RATS; REPRODUCIBILITY OF RESULTS;

EID: 84875509584     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0059727     Document Type: Article

References (12)

1. Kang J, Lee MS, Gorenstein DG, (2005) Quantitative analysis of chemiluminescence signals using a cooled charge-coupled device camera. Anal Biochem 345: 66-71.
2. Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, et al. (2005) High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307: 1621-1625.
3. Schulte R, Talamas J, Doucet C, Hetzer MW, (2008) Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. PLoS One 3: e2061.
4. Patel SS, Rexach MF, (2008) Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics 7: 121-131.
5. Wu M, Lu L, Hong W, Song H, (2004) Structural basis for recruitment of GRIP domain golgin-245 by small GTPase Arl1. Nat Struct Mol Biol 11: 86-94.
6. Lu L, Hong W, (2003) Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi. Molecular biology of the cell 14: 3767-3781.
7. Teuchert M, Schafer W, Berghofer S, Hoflack B, Klenk HD, et al. (1999) Sorting of furin at the trans-Golgi network. Interaction of the cytoplasmic tail sorting signals with AP-1 Golgi-specific assembly proteins. J Biol Chem 274: 8199-8207.
8. Teuchert M, Berghofer S, Klenk HD, Garten W, (1999) Recycling of furin from the plasma membrane. Functional importance of the cytoplasmic tail sorting signals and interaction with the AP-2 adaptor medium chain subunit. J Biol Chem 274: 36781-36789.
9. Thomas G, (2002) Furin at the cutting edge: from protein traffic to embryogenesis and disease. Nat Rev Mol Cell Biol 3: 753-766.
10. Bonifacino JS, Traub LM, (2003) Signals for sorting of transmembrane proteins to endosomes and lysosomes. Annu Rev Biochem 72: 395-447.
11. Lu L, Horstmann H, Ng C, Hong W, (2001) Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J Cell Sci 114: 4543-4555.
12. Kelso RJ, Buszczak M, Quinones AT, Castiblanco C, Mazzalupo S, et al. (2004) Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Res 32: D418-420.