A high-throughput mass spectrometry assay coupled with redox activity testing reduces artifacts and false positives in lysine demethylase screening

Journal of Biomolecular Screening

Volumn 20, Issue 6, 2015, Pages 810-820

  Wigle, Tim J ^a   Swinger, Kerren K ^a   Campbell, John E ^a   Scholle, Michael D ^b   Sherrill, John ^b   Admirand, Elizabeth A ^a   Boriack Sjodin, P Ann ^a   Kuntz, Kevin W ^a   Chesworth, Richard ^a   Moyer, Mikel P ^a   Scott, Margaret Porter ^a   Copeland, Robert A ^a  

^a EPIZYME INC   (United States)

^b SAMDI Tech   (United States)

Author keywords

epigenetics; KDM1A or LSD1 or AOF2; KDM4C or JMJD2C or GASC1; lysine demethylase (KDM); redox; self assembled monolayer desorption ionization mass spectrometry (SAMDI)

Indexed keywords

2 OXOGLUTARIC ACID; 4 CARBOXY 2,2' DIPYRIDINE; ENZYME INHIBITOR; EPZ 020809; HISTONE DEMETHYLASE; LYSINE DEMETHYLASE; LYSINE DEMETHYLASE INHIBITOR; LYSINE SPECIFIC DEMETHYLASE 1; LYSINE SPECIFIC DEMETHYLASE 4C; N OXAYLGLYCINE; PROTEIN AOF2; PROTEIN GASC1; PROTEIN JMJD2C; PROTEIN LSD1; PYRIDINE DERIVATIVE; TRANYLCYPROMINE; UNCLASSIFIED DRUG; HISTONE; KDM1A PROTEIN, HUMAN; KDM4C PROTEIN, HUMAN; MOLECULAR LIBRARY;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DEMETHYLATION; DRUG SCREENING; ENZYME INHIBITION; ENZYME KINETIC ASSAY; HIGH THROUGHPUT SCREENING; HUMAN; MASS SPECTROMETRY; OXIDATION; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SELF ASSEMBLED DESORPTION IONIZATION MASS SPECTROMETRY; SENSITIVITY ANALYSIS; SIGNAL DETECTION; ARTIFACT; DRUG DEVELOPMENT; ENZYME ASSAY; METABOLISM; METHYLATION; MOLECULAR LIBRARY; PROCEDURES;

ARTIFACTS; DRUG DISCOVERY; ENZYME ASSAYS; HIGH-THROUGHPUT SCREENING ASSAYS; HISTONE DEMETHYLASES; HISTONES; HUMANS; JUMONJI DOMAIN-CONTAINING HISTONE DEMETHYLASES; MASS SPECTROMETRY; METHYLATION; OXIDATION-REDUCTION; SMALL MOLECULE LIBRARIES;

EID: 84931426731     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057115575689     Document Type: Article

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