High-resolution cytometry for high-content cell cycle analysis

Current Protocols in Cytometry

Volumn 2014, Issue , 2014, Pages 7.41.1-7.41.15

  Furia, Laura ^a   Pelicci, Piergiuseppe ^a   Faretta, Mario ^a  

^a EUROPEAN INSTITUTE OF ONCOLOGY   (Italy)

Author keywords

Automated microscopy; Cell cycle; High resolution; High content analysis; Image cytometry; S phase; Wide field microscopy

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; FLUOROCHROME;

ARTICLE; AUTOMATION; CELL CYCLE; CELL LABELING; COMPUTER PROGRAM; CYTOMETRY; FLOW CYTOMETRY; HIGH RESOLUTION CYTOMETRY; IMAGE CYTOMETRY; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; INFORMATION PROCESSING; MICROSCOPY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; TIME LAPSE IMAGING; ANIMAL; DEVICES; HUMAN; PROCEDURES;

ANIMALS; AUTOMATION; CELL CYCLE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY;

EID: 84931384627     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0741s70     Document Type: Article

References (21)

1. Basiji, D.A., Ortyn, W.E., Liang, L., Venkatachalam, V., and Morrissey, P. 2007. Cellular image analysis and imaging by flow cytometry. Clinics Lab. Med. 27:653-670.
2. Bernaś, T., Berniak, K., Rybak, P., Zarȩbski, M., Zhao, H., Darzynkiewicz, Z., and Dobrucki, J.W. 2013. Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage. Cytometry A 83:825-932.
3. Berniak, K., Rybak, P., Bernaś, T., Zarȩbski, M., Biela, E., Zhao, H., Darzynkiewicz, Z., and Dobrucki, J.W. 2013. Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis. Cytometry A 83:824-913.
4. Buck, S.B., Bradford, J., Gee, K.R., Agnew, B.J., Clarke, S.T., and Salic, A. 2008. Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies. BioTechniques 44:927-929.
5. Chattopadhyay, P.K., Hogerkorp, C.M., and Roederer, M. 2008. A chromatic explosion: The development and future of multiparameter flowcytometry. Immunology 125:441-449.
6. Conchello, J.A. and Lichtman, J.W. 2005. Optical sectioning microscopy. Nat. Methods 2:920-931.
7. Darzynkiewicz, Z., Traganos, F., and Melamed M.R. 1980. New cell cycle compartments identified by multiparameter flow cytometry. Cytometry 1:98-108.
8. Darzynkiewicz, Z., Zhao, H., Halicka, H.D., and Li, J. 2011a. Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "Click chemistry". Cytometry A 79:328-337.
9. Darzynkiewicz, Z., Traganos, F., Zhao, H., Halicka, H.D., Skommer, J., and Wlodkowic, D. 2011b. Analysis of individual molecular events of DNA damage response by flow- and image-assisted cytometry. Methods Cell Biol. 103:115-147.
10. Furia, L., Pelicci, P.G., and Faretta, M. 2013a. A computational platform for robotized fluorescence microscopy (I): High-content imagebased cell-cycle analysis. Cytometry A 83:333-343.
11. Furia, L., Pelicci, P.G., and Faretta, M. 2013b. A computational platform for robotized fluorescence microscopy (II): DNA damage, replication, checkpoint activation, and cell cycle progression by high-content high-resolution multiparameter image-cytometry. Cytometry A 83:344-355.
12. Hell, S.W. 2007. Far-field optical nanoscopy. Science 316:1153-1158.
13. Kamentsky, L.A. 2001. Laser scanning cytometry. Methods Cell Biol. 63:51-87.
14. Li, X., Melamed, M.R., and Darzynkiewicz, Z. 1996. Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp. Cell Res. 222:28-37.
15. Loo, L.H., Wu, L.F., and Altschuler, S.J. 2007. Image-based multivariate profiling of drug responses from single cells. Nat. Methods 4:445-453.
16. Mach, W.J., Thimmesch, A.R., Orr, J.A., Slusser, J.G., and Pierce, J.D. 2010. Flow cytometry and laser scanning cytometry, a comparison of techniques. J. Clin. Monitor. Comput. 24:251-259.
17. McCoy, J.P. Jr. 2011. High-content screening: Getting more from less. Nat. Methods 8:390-391.
18. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wunsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R., and Ellenberg, J. 2010. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464:721-727.
19. Ortyn, W.E., Perry, D.J., Venkatachalam, V., Liang, L., Hall, B.E., Frost, K., and Basiji, D.A. 2007. Extended depth of field imaging for high speed cell analysis. Cytometry A 71:215-231.
20. Pawley, J.B. 1995. Handbook of Biological Confocal Microscopy. Plenum, New York.
21. Salic, A. and Mitchison, T.J. 2008. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. U.S.A. 105:2415-2420.