Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage

Cytometry Part A

Volumn 83, Issue 10, 2013, Pages 925-932

  Bernas, Tytus ^a,b   Berniak, Krzysztof ^a   Rybak, Paulina ^a   Zarebski, Miroslaw ^a   Zhao, Hong ^c   Darzynkiewicz, Zbigniew ^c   Dobrucki, Jerzy W ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b NENCKI INSTITUTE OF EXPERIMENTAL BIOLOGY   (Poland)

^c NEW YORK MEDICAL COLLEGE   (United States)

Author keywords

H2AX; Camptothecin; Cell cycle; Correlation analysis; DDR; DNA damage; DNA damage response; DNA replication; Histone H2AX phosphorylation; Hydrogen peroxide; Oxidative stress

Indexed keywords

CAMPTOTHECIN; DNA TOPOISOMERASE INHIBITOR; H2AFX PROTEIN, HUMAN; HISTONE;

CELL CULTURE; CONFOCAL MICROSCOPY; DNA DAMAGE; DNA REPLICATION; DRUG EFFECTS; HUMAN; IMAGE PROCESSING; METABOLISM; OXIDATIVE STRESS; PHYSIOLOGY; PROCEDURES; THREE DIMENSIONAL IMAGING;

CAMPTOTHECIN; CELLS, CULTURED; DNA DAMAGE; DNA REPLICATION; HISTONES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; OXIDATIVE STRESS; TOPOISOMERASE I INHIBITORS;

EID: 84884989585     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22325     Document Type: Article

References (13)

1. Berniak K, Rybak P, Bernaś T, Zarebski M, Biela E, Zhao H, Darzynkiewicz Z, Dobrucki JW. Relationship between DNA Damage Response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative image analysis. Cytometry A 2013;83A:913-924.
2. Zhao H, Dobrucki J, Rybak P, Traganos F, Halicka DH, Darzynkiewicz Z. Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using "click chemistry." Cytometry A 2011;79A:897-902.
3. Zhao H, Li J, Traganos F, Halicka HD, Zarebski M, Dobrucki J, Darzynkiewicz Z. Cell fixation in zinc salt solution is compatible with DNA damage response detection by phospho-specific antibodies. Cytometry A 2011;79A:470-476.
4. Huang X, Okafuji M, Traganos F, Luther E, Holden E, Darzynkiewicz Z. Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by the DNA cross-linking agent cisplatin. Cytometry A 2004;58A:99-110.
5. Zhao H, Rybak P, Dobrucki J, Traganos F, Darzynkiewicz Z. Relationship of DNA damage signaling to DNA replication following treatment with DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone, or etoposide. Cytometry A 2012;81A:45-51.
6. Ripley BD. The second-order analysis of stationary point process. J Appl Prob 1976;13:255-266.
7. Bailey TC, Gatrell T. Interactive Spatial Data Analysis. Harlow, Essex, England: Longman Scientific & Technical; 1995.
8. Umande PP, D. A. Analysis of promyelocytic leukemia nuclear bodies. In: Adams NM, Freemont PS, editors. Advances in Nuclear Architecture. Dordrecht, Netherlands: Springer Science and Business Media B.V.; 2011.
9. Veatch SL, Machta BB, Shelby SA, Chiang EN, Holowka DA, Baird BA. Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting. PLoS One 2012;7:e31457.
10. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 2006;5:1940-1945.
11. Cleaver JE. γH2Ax: Biomarker of damage or functional participant in DNA repair "all that glitters is not gold!". Photochem Photobiol 2011;87:1230-1239.
12. Furia L, Pelicci PG, Faretta M. A computational platform for robotized fluorescence microscopy (I): High-content image-based cell-cycle analysis. Cytometry A 2013;83A:333-343.
13. Furia L, Pelicci PG, Faretta M. A computational platform for robotized fluorescence microscopy (II): DNA damage, replication, checkpoint activation, and cell cycle progression by high-content high-resolution multiparameter image-cytometry. Cytometry A. 2013;83A:344-355.