Nondestructive evaluation of progressive neuronal changes in organotypic rat hippocampal slice cultures using ultrahigh-resolution optical coherence microscopy

Neurophotonics

Volumn 1, Issue 2, 2014, Pages

  Li, Fengqiang ^a,b   Song, Yu ^a   Dryer, Alexandra ^a   Cogguillo, William ^a   Berdichevsky, Yevgeny ^a,b   Zhou, Chao ^a,b  

^a Lehigh University   (United States)

^b LEHIGH UNIVERSITY   (United States)

Author keywords

image segmentation; neuron death; optical coherence microscopy; organotypic

Indexed keywords

CELL DEATH; CELLS; CYTOLOGY; DIAGNOSIS; NEURONS; NONDESTRUCTIVE EXAMINATION; RATS; THROUGHPUT; TISSUE; TISSUE CULTURE;

HIGH THROUGHPUT SCREENING; HIPPOCAMPAL CULTURE; NON DESTRUCTIVE EVALUATION; NON-DESTRUCTIVE TECHNIQUE; OPTICAL COHERENCE MICROSCOPY; ORGANOTYPIC; TRANSVERSE RESOLUTION; ULTRAHIGH RESOLUTION;

IMAGE SEGMENTATION;

KYNURENIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CELL COUNT; CONTROLLED STUDY; HIPPOCAMPUS; IN VITRO STUDY; MICROSCOPY; NERVE CELL LESION; NONHUMAN; OPTICAL COHERENCE MICROSCOPY; OPTICAL RESOLUTION; RAT; SEIZURE; SLICE CULTURE; TISSUE CULTURE;

EID: 84930814119     PISSN: 2329423X     EISSN: 23294248     Source Type: Journal    
DOI: 10.1117/1.NPh.1.2.025002     Document Type: Article

References (31)

1. J. H. Sung, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab on a Chip. 13(7), 1201-1212 (2013
2. A. M. Ghaemmaghami, et al. Biomimetic tissues on a chip for drug discovery. Drug Discov. Today 17(3-4), 173-181 (2012
3. B. H. Gahwiler, et al. Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20(10), 471-477 (1997
4. Y. Berdichevsky, et al. PI3K-Akt signaling activates mTOR-mediated epileptogenesis in organotypic hippocampal culture model of post-Traumatic epilepsy. J. Neurosci. 33(21), 9056-9067 (2013
5. W. Loscher and C. Brandt, "Prevention or modification of epileptogenesis after brain insults: experimental approaches and translational research. Pharmacol. Rev. 62(4), 668-700 (2010
6. Y. Berdichevsky, et al. Interictal spikes, seizures and ictal cell death are not necessary for post-Traumatic epileptogenesis in vitro. Neurobiol. Dis. 45(2), 774-785 (2012
7. D. Huang, et al. Optical coherence tomography. Science 254(5035), 1178-1181 (1991
8. A. D. Aguirre, et al. Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography. Opt. Lett. 31(23), 3459-3461 (2006
9. Y. Chen, et al. Optical coherence tomography (OCT) reveals depthresolved dynamics during functional brain activation. J. Neurosci. Methods 178(1), 162-173 (2009
10. V. J. Srinivasan, et al. OCT methods for capillary velocimetry. Biomed. Opt. Express 3(3), 612-629 (2012
11. J. Lee, et al. Quantitative imaging of cerebral blood flow velocity and intracellular motility using dynamic light scattering-optical coherence tomography. J. Cereb. Blood Flow Metab. 33(6), 819-825 (2013
12. J. Lee, et al. Dynamic light scattering optical coherence tomography. Opt. Express 20(20), 22262-22277 (2012
13. J. A. Izatt, et al. Optical coherence microscopy in scattering media. Opt. Lett. 19(8), 590-592 (1994
14. A. D. Aguirre, et al. High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging. Opt. Lett. 28(21), 2064-2066 (2003
15. A. D. Aguirre, et al. High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe. Opt. Express 18(5), 4222-4239 (2010
16. C. Zhou, et al. Integrated optical coherence tomography and microscopy for ex vivo multiscale evaluation of human breast tissues. Cancer Res. 70(24), 10071-10079 (2010
17. C. Zhou, et al. Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy. J. Biomed. Opt. 15(1), 016001 (2010
18. H. C. Lee, et al. Integrated optical coherence tomography and optical coherence microscopy imaging of ex vivo human renal tissues. J. Urol. 187(2), 691-699 (2012
19. H. C. Lee, et al. Ultrahigh speed spectral-domain optical coherence microscopy. Biomed. Opt. Express 4(8), 1236-1254 (2013
20. O. O. Ahsen, et al. Swept source optical coherence microscopy using a 1310 nm VCSEL light source. Opt. Express 21(15), 18021-18033 (2013
21. F. Li, et al. Label-free evaluation of angiogenic sprouting in microengineered devices using ultrahigh-resolution optical coherence microscopy. J. Biomed. Opt. 19(1), 016006 (2014
22. T. Bolmont, et al. Label-free imaging of cerebral beta-Amyloidosis with extended-focus optical coherence microscopy. J. Neurosci. 32(42), 14548-14556 (2012
23. C. Leahy, H. Radhakrishnan, and V. J. Srinivasan, "Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast. Biomed. Opt. Express 4(10), 1978-1990 (2013
24. V. J. Srinivasan, et al. Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast. Opt. Express. 20(3), 2220-2239 (2012
25. T. W. Stone, "Neuropharmacology of quinolinic and kynurenic acids. Pharmacol. Rev. 45(3), 309-379 (1993
26. A. C. Foster, et al. Kynurenic acid blocks neurotoxicity and seizures induced in rats by the related brain metabolite quinolinic acid. Neurosci. Lett. 48(3), 273-278 (1984
27. D. Zadori, et al. Neuroprotective effects of a novel kynurenic acid analogue in a transgenic mouse model of Huntington's disease. J. Neural Transm. 118(6), 865-875 (2011
28. R. A. Leitgeb, et al. Extended focus depth for Fourier domain optical coherence microscopy. Opt. Lett. 31(16), 2450-2452 (2006
29. M. Oberlaender, et al. Automated three-dimensional detection and counting of neuron somata. J. Neurosci. Methods 180(1), 147-160 (2009
30. W. Choi, et al. Tomographic phase microscopy. Nat. Methods 4(9), 717-719 (2007
31. L. D. P. Miller, et al. Spontaneous pyramidal cell-death in organotypic slice cultures from rat hippocampus is prevented by glutamate-receptor antagonists. Neuroscience 63(2), 471-487 (1994