Thermoadaptation-directed evolution of chloramphenicol acetyltransferase in an error-prone thermophile using improved procedures

Applied Microbiology and Biotechnology

Volumn 99, Issue 13, 2015, Pages 5563-5572

  Kobayashi, Jyumpei ^a,b   Furukawa, Megumi ^b   Ohshiro, Takashi ^a   Suzuki, Hirokazu ^a,b  

^a TOTTORI UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Chloramphenicol acetyltransferase; Chloramphenicol resistance; Error prone thermophile; Geobacillus kaustophilus; Thermoadaptation directed evolution

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYST ACTIVITY; ENCODING (SYMBOLS); ENZYMES; GENE ENCODING; GENES; STABILITY;

CHLORAMPHENICOL ACETYLTRANSFERASE; DIRECTED EVOLUTION; ENZYMATIC PROPERTIES; ERROR PRONES; GEOBACILLUS KAUSTOPHILUS; PROTEIN THERMOSTABILITIES; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS AUREUS;

ANTIBIOTICS;

BACTERIAL ENZYME; CHLORAMPHENICOL ACETYLTRANSFERASE; RECOMBINANT PROTEIN; MUTANT PROTEIN;

ADAPTATION; BACTERIUM; ENZYME ACTIVITY; EVOLUTIONARY BIOLOGY; HIGH TEMPERATURE; MUTAGENICITY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; GEL FILTRATION; GENETIC MARKER; GEOBACILLUS; GEOBACILLUS KAUSTOPHILUS; MOLECULAR EVOLUTION; MUTATION RATE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS AUREUS; TEMPERATURE ACCLIMATIZATION; THERMOSTABILITY; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; METABOLISM; MICROBIAL GENETICS; MISSENSE MUTATION; PROCEDURES; PROTEIN STABILITY; RADIATION RESPONSE; TEMPERATURE;

GEOBACILLUS; GEOBACILLUS KAUSTOPHILUS; STAPHYLOCOCCUS AUREUS;

CHLORAMPHENICOL O-ACETYLTRANSFERASE; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GENETICS, MICROBIAL; GEOBACILLUS; MUTANT PROTEINS; MUTATION, MISSENSE; PROTEIN STABILITY; RECOMBINANT PROTEINS; STAPHYLOCOCCUS AUREUS; TEMPERATURE;

EID: 84930757423     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6522-4     Document Type: Article

References (31)

1. Asako H, Shimizu M, Itoh N (2008) Engineering of NADPH-dependent aldo-keto reductase from Penicillium citrinum by directed evolution to improve thermostability and enantioselectivity. Appl Microbiol Biotechnol 80(5):805–812. doi:10.1007/s00253-008-1594-z
2. Ben Mabrouk S, Ayadi DZ, Ben Hlima H, Bejar S (2013) Thermostability improvement of maltogenic amylase MAUS149 by error prone PCR. J Biotechnol 168(4):601–606. doi:10.1016/j.jbiotec.2013.08.026
3. I. Protein Sci 21(4):520–530. doi:10.1002/pro.2036
4. Bloom JD, Meyer MM, Meinhold P, Otey CR, MacMillan D, Arnold FH (2005) Evolving strategies for enzyme engineering. Curr Opin Struct Biol 15(4):447–452. doi:10.1016/j.sbi.2005.06.004
5. Brouns SJJ, Wu H, Akerboom J, Turnbull AP, de Vos WM, van der Oost J (2005) Engineering a selectable marker for hyperthermophiles. J Biol Chem 280(12):11422–11431. doi:10.1074/jbc.M413623200
6. Drummond DA, Iverson BL, Georgiou G, Arnold FH (2005) Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins. J Mol Biol 350(4):806–1816. doi:10.1016/j.jmb.2005.05.023
7. Fang ZM, Zhou P, Chang F, Yin Q, Fang W, Yuan J, Zhang X, Xiao Y (2014) Structure-based rational design to enhance the solubility and thermostability of a bacterial laccase Lac15. PLoS One 9(7):e102423. doi:10.1371/journal.pone.0102423
8. Fridjonsson O, Watzlawick H, Mattes R (2002) Thermoadaptation of α-galactosidase AgaB1 in Thermus thermophilus. J Bacteriol 184(12):3385–3391. doi:10.1128/JB.184.12.3385-3391.2002
9. Hoseki J, Yano T, Koyama Y, Kuramitsu S, Kagamiyama H (1999) Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J Biochem 126(5):951–956
10. Huang J, Cheng Y, Ko T, Lin C, Lai H, Chen C, Ma Y, Zheng Y, Huang C, Zou P, Liu J, Guo R (2012) Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-D-glucanase. Appl Microbiol Biotechnol 94(1):111–121. doi:10.1007/s00253-011-3586-7
11. Imanaka T, Fujii M, Aramori I, Aiba S (1982) Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis. J Bacteriol 149(3):824–830
12. Labrou NE (2010) Random mutagenesis methods for in vitro directed enzyme evolution. Curr Protein Pept Sci 11(1):91–100. doi:10.2174/1389209198868622037
13. Leslie AGW (1990) Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 Å resolution. J Mol Biol 213(1):167–186. doi:10.1016/s0022-2836(05)80129-9
14. Leslie AGW, Moody PCE, Shaw WV (1988) Structure of chloramphenicol acetyltransferase at 1.75-Å resolution. Proc Natl Acad Sci U S A 85(12):4133–4137. doi:10.1073/pnas.85.12.4133
15. Liao H, McKenzie T, Hageman R (1986) Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc Natl Acad Sci U S A 83(3):576–580. doi:10.1073/pnas.83.3.576
16. Mankin AS, Garrett RA (1991) Chloramphenicol resistance mutations in the single 23S rRNA gene of the archaeon Halobacterium halobium. J Bacteriol 173(11):3559–3563
17. Matsumura M, Aiba S (1985) Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus. J Biol Chem 260(28):15298–15303
18. McMurry LM, George AM, Levy SB (1994) Active efflux of chloramphenicol in susceptible Escherichia coli strains and in multiple-antibiotic-resistant (Mar) mutants. Antimicrob Agents Chemother 38(3):542–546. doi:10.1128/AAC.38.3.542
19. Nakamura A, Takakura Y, Kobayashi H, Hoshino T (2005) In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Thermus thermophilus. J Biosci Bioeng 100(2):158–163. doi:10.1263/jbb.100.158
20. Rhee MS, Kim J, Qian Y, Ingram LO, Shanmugam KT (2007) Development of plasmid vector and electroporation condition for gene transfer in sporogenic lactic acid bacterium, Bacillus coagulans. Plasmid 58(1):13–22. doi:10.1016/j.plasmid.2006.11.006
21. Shaw WV, Brodsky RF (1968) Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. J Bacteriol 95(1):28–36
22. Stephens DE, Khan FI, Singh P, Bisetty K, Singh S, Permaul K (2014) Creation of thermostable and alkaline stable xylanase by DNA shuffling. J Biotechnol 187:139–146. doi:10.1016/j.jbiotec.2014.07.446
23. Suzuki H, Yoshida K (2012) Genetic transformation of Geobacillus kaustophilus HTA426 by conjugative transfer of host-mimicking plasmids. J Microbiol Biotechnol 22(9):1279–1287. doi:10.4014/jmb.1203.03023
24. Suzuki H, Murakami A, Yoshida K (2012) Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR. Appl Environ Microbiol 78(20):7376–7383. doi:10.1128/aem. 01669-12
25. Suzuki H, Wada K, Furukawa M, Doi K, Ohshima T (2013a) A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426. Biosci Biotechnol Biochem 77(11):2316–2318. doi:10.1271/bbb.1304921
26. Suzuki H, Yoshida K, Ohshima T (2013b) Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426. Appl Environ Microbiol 79(17):5151–5158. doi:10.1128/AEM. 01506-13
27. Suzuki H, Kobayashi J, Wada K, Furukawa M, Doi K (2015) Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426. Appl Environ Microbiol 81(1):149–158. doi:10.1128/AEM. 02577-14
28. Takami H, Takaki Y, Chee G-J, Nishi S, Shimamura S, Suzuki H, Matsui S, Uchiyama I (2004) Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus. Nucleic Acids Res 32(21):6292–6303. doi:10.1093/nar/gkh970
29. Tamakoshi M, Nakano Y, Kakizawa S, Yamagishi A, Oshima T (2001) Selection of stabilized 3-isopropylmalate dehydrogenase of Saccharomyces cerevisiae using the host-vector system of an extreme thermophile, Thermus thermophilus. Extremophiles 5(1):17–22. doi:10.1007/s007920000168
30. Taylor MP, Esteban CD, Leak DJ (2008) Development of a versatile shuttle vector for gene expression in Geobacillus spp. Plasmid 60(1):45–52. doi:10.1016/j.plasmid.2008.04.001
31. Wu L, Welker NE (1989) Protoplast transformation of Bacillus stearothermophilus NUB36 by plasmid DNA. J Gen Microbiol 135(5):1315–1324. doi:10.1099/00221287-135-5-1315