Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-d-glucanase

Applied Microbiology and Biotechnology

Volumn 94, Issue 1, 2012, Pages 111-121

  Huang, Jian Wen ^a,b   Cheng, Ya Shan ^c   Ko, Tzu Ping ^d   Lin, Cheng Yen ^a,b   Lai, Hui Lin ^a,b   Chen, Chun Chi ^e   Ma, Yanhe ^g   Zheng, Yingying ^g   Huang, Chun Hsiang ^g   Zou, Peijian ^g   Liu, Je Ruei ^c,f   Guo, Rey Ting ^g  

^a Genozyme Biotechnology Inc   (Taiwan)

^b AsiaPac Biotechnology Co Ltd   (China)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^d INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^e INSTITUTE OF BIOMEDICAL SCIENCES   (Taiwan)

^f AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

^g Chinese Academy of Sciences   (China)

Author keywords

Glucanase; Cellobiose; Cellotetraose; Crystal structure; Synchrotron radiation

Indexed keywords

CELLOBIOSE; CELLOTETRAOSE; CRUDE PROTEINS; DOUBLE MUTANTS; E. COLI; ENZYME STABILITY; FEED ADDITIVES; FIBROBACTER SUCCINOGENES; GLUCANASE; INDUSTRIAL FERMENTATION; P. PASTORIS; PICHIA PASTORIS; PLANT FIBERS; PROTEIN PRODUCTION; PROTEIN STABILITY; PROTEIN STRUCTURES; RATIONAL DESIGN; SIMILAR PATTERN; SINGLE MUTANT; SPECIFIC ACTIVITY; STRUCTURAL POINT; STRUCTURE-BASED; STRUCTURE-FUNCTION RELATIONSHIP; SUBSTRATE COMPLEXES; TURNOVER RATE; WILD-TYPE PROTEINS;

ANIMALS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; INDUSTRIAL APPLICATIONS; PURIFICATION; SYNCHROTRON RADIATION; X RAY CRYSTALLOGRAPHY;

PROTEINS;

ENDO 1,3(4) BETA GLUCANASE; FUNGAL PROTEIN;

ANIMAL; BACTERIUM; CRYSTAL STRUCTURE; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; PROTEIN; TEMPERATURE TOLERANCE; YEAST;

ARTICLE; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FERMENTATION; FIBROBACTER SUCCINOGENES; MUTATION; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION; THERMOTOGA MARITIMA; WILD TYPE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; ENDO-1,3(4)-BETA-GLUCANASE; ENZYME STABILITY; FIBROBACTER; HOT TEMPERATURE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PICHIA; PROTEIN ENGINEERING;

ANIMALIA; ESCHERICHIA COLI; FIBROBACTER SUCCINOGENES; PICHIA PASTORIS;

EID: 84858451153     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3586-7     Document Type: Article

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