Deconstructing the polymerase chain reaction: Understanding and correcting bias associated with primer degeneracies and primer-template mismatches

PLoS ONE

Volumn 10, Issue 5, 2015, Pages

  Green, Stefan J ^a   Venkatramanan, Raghavee ^a,b   Naqib, Ankur ^a,b  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b University of Illinois at Chicago   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; EXONUCLEASE; GENOMIC DNA; PRIMER DNA;

AMPLICON; ARTICLE; BINDING SITE; CHEMICAL INTERACTION; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; HIGH TEMPERATURE; LOW TEMPERATURE; MELTING POINT; MICROBIAL COMMUNITY; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; BASE MISPAIRING; PROCEDURES;

BASE PAIR MISMATCH; DNA PRIMERS; POLYMERASE CHAIN REACTION;

EID: 84930672461     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0128122     Document Type: Article

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