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Volumn 5, Issue 55, 2015, Pages 44530-44544

Enzyme immobilization on silicate glass through simple adsorption of dendronized polymer-enzyme conjugates for localized enzymatic cascade reactions

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; CATALYST ACTIVITY; CHAINS; COPOLYMERS; ENZYMES; GLASS; GLUCOSE OXIDASE; GLUCOSE SENSORS; POLYMERS; SILICATES;

EID: 84930221651     PISSN: None     EISSN: 20462069     Source Type: Journal    
DOI: 10.1039/c5ra06268c     Document Type: Article
Times cited : (38)

References (73)
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    • In that work a partially biotinylated PLL-g-PEG (with about 75-80 free amines per polymer chain) was used with neutrAvidin for the immobilization on niobium oxide (Nb2O5) of a genetically engineered β-lactamase which was biotinylated specifically
    • G. Zhen V. Eggli J. Vörös P. Zammaretti M. Textor R. Glockshuber E. Kuennemann et al. Langmuir 2004 20 10464 10473
    • (2004) Langmuir , vol.20 , pp. 10464-10473
    • Zhen, G.1    Eggli, V.2    Vörös, J.3    Zammaretti, P.4    Textor, M.5    Glockshuber, R.6    Kuennemann, E.7
  • 54
    • 84930219716 scopus 로고    scopus 로고
    • http://www.toyobo-global.com/seihin/xr/enzyme/e-top.html
  • 55
    • 84930219717 scopus 로고    scopus 로고
    • S-HyNic and S-4FB are commercially available from Solulink, Inc., San Diego, California
    • S-HyNic and S-4FB are commercially available from Solulink, Inc., San Diego, California, http://www.solulink.com
  • 68
    • 72049090637 scopus 로고    scopus 로고
    • HRP was covalently bound to polymer brushes which were first grown from a modified silicon wafer, yielding a surface density of up to ≈4 pmol cm-2 (2.6 × 1012 HRP molecules per cm2)
    • P. Ye R.-B. Wan X.-P. Wang J. Mol. Catal. B: Enzym. 2009 61 296 302
    • (2009) J. Mol. Catal. B: Enzym. , vol.61 , pp. 296-302
    • Ye, P.1    Wan, R.-B.2    Wang, X.-P.3
  • 70
    • 84967495945 scopus 로고    scopus 로고
    • Imperial College Press, London, Calculated on the basis of the following: a micropipette cut to a length of 7 cm contained a volume of 140 μL. By assuming the shape of a perfect cylinder, this gives a total surface of 3.5 cm2 per micropipette
    • P. Grunwald, Biocatalysis: Biochemical Fundamentals and Applications, Imperial College Press, London, 2009
    • (2009) Biocatalysis: Biochemical Fundamentals and Applications
    • Grunwald, P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.