Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Gao, Fan ^a,b   Wang, Kai ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Blood; Immune repertoire; Next generation sequencing

Indexed keywords

BLOOD; CELL MEMBRANES; T-CELLS;

ADAPTIVE IMMUNITY; GENETIC MECHANISM; HIGH-THROUGHPUT SEQUENCING; IMMUNE REPERTOIRE; NEXT-GENERATION SEQUENCING; SYSTEMATIC EXPLORATION; T CELL RECEPTOR; VARIABLE REGIONS;

POLYMERASE CHAIN REACTION;

IMMUNOGLOBULIN; RNA; T LYMPHOCYTE RECEPTOR BETA CHAIN; IMMUNOGLOBULIN VARIABLE REGION; LYMPHOCYTE ANTIGEN RECEPTOR; PRIMER DNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; FEASIBILITY STUDY; GENE AMPLIFICATION; GENE LIBRARY; GENE MAPPING; GENETIC VARIABILITY; GENOME SIZE; HUMAN; IMMUNE RESPONSE; IMMUNOGENETICS; LIGATION ANCHORED POLYMERASE CHAIN REACTION; MALIGNANT MENINGIOMA; MENINGIOMA; NEXT GENERATION SEQUENCING; NUCLEIC ACID ANALYSIS; RNA EXTRACTION; VDJ RECOMBINATION; BLOOD; FEMALE; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MALE; NUCLEOTIDE SEQUENCE; PATHOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES; T LYMPHOCYTE;

BASE SEQUENCE; DNA PRIMERS; FEMALE; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MALE; MENINGIOMA; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; RNA; T-LYMPHOCYTES;

EID: 84930207446     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0153-9     Document Type: Article

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