Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein

Biotechnology and Bioengineering

Volumn 112, Issue 7, 2015, Pages 1297-1305

  Beiss, Veronique ^a   Spiegel, Holger ^a   Boes, Alexander ^a   Kapelski, Stephanie ^a   Scheuermayer, Matthias ^b   Edgue, Gueven ^a   Sack, Markus ^c   Fendel, Rolf ^a   Reimann, Andreas ^a   Schillberg, Stefan ^a   Pradel, Gabriele ^b,c   Fischer, Rainer ^a,c  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Agroinfiltration; Downstream processing; Host cell protein removal; Plant made vaccine; Plasmodium falciparum

Indexed keywords

COST EFFECTIVENESS; DEVELOPING COUNTRIES; DISEASE CONTROL; DISEASES; LIFE CYCLE; PROTEINS; PURIFICATION; RECOMBINANT PROTEINS; VACCINES;

AGROINFILTRATION; DOWNSTREAM-PROCESSING; HOST CELL PROTEIN; PLANT-MADE VACCINES; PLASMODIUM FALCIPARUM;

PLANTS (BOTANY);

HYBRID PROTEIN; MALARIA TRANSMISSION BLOCKING VACCINE; MALARIA VACCINE; UNCLASSIFIED DRUG; PARASITE ANTIGEN; PFS230 ANTIGEN, PLASMODIUM FALCIPARUM; PFS25 PROTEIN, PLASMODIUM FALCIPARUM; PROTOZOAL PROTEIN;

AGROBACTERIUM TUMEFACIENS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY TITER; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DEVELOPING COUNTRY; DRUG DETERMINATION; GAMETOCYTE; GENE EXPRESSION SYSTEM; GENE FUSION; HEAT TREATMENT; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIFE CYCLE; MALARIA; MOUSE; NICOTIANA BENTHAMIANA; NONHUMAN; PLANT LEAF; PLASMODIUM FALCIPARUM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRODUCT SAFETY; PROTEIN PURIFICATION; TRANSIENT EXPRESSION; ANIMAL; GENETICS; HEAT; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PRECIPITATION; PROCEDURES; TOBACCO; TRANSGENIC PLANT; VACCINATION;

EUKARYOTA; MUS; NICOTIANA BENTHAMIANA; PLASMODIUM FALCIPARUM; TULIP BREAKING VIRUS;

ANIMALS; ANTIGENS, PROTOZOAN; FRACTIONAL PRECIPITATION; HOT TEMPERATURE; MALARIA VACCINES; MICE; PLANTS, GENETICALLY MODIFIED; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; TOBACCO; VACCINATION;

EID: 84929951763     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25548     Document Type: Article

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