A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

Applied Microbiology and Biotechnology

Volumn 99, Issue 12, 2015, Pages 5151-5162

  Sun, Zhaopeng ^a,b   Deng, Aihua ^a   Hu, Ting ^a   Wu, Jie ^a,b   Sun, Qinyun ^a,b   Bai, Hua ^a,b   Zhang, Guoqiang ^a   Wen, Tingyi ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Bacillus subtilis; PCR based ssDNA; Recombinase; Recombineering frequency

Indexed keywords

DNA; ESCHERICHIA COLI; GENES; MACHINERY; POLYMERASE CHAIN REACTION;

BACILLUS SUBTILIS; PCR-BASED SSDNA; PURINE NUCLEOSIDE PHOSPHORYLASE; RECOMBINASE; RECOMBINATION ACTIVITY; RECOMBINATION MACHINERY; RECOMBINEERING; URACIL PHOSPHORIBOSYLTRANSFERASE;

BACTERIOLOGY;

RECOMBINASE; RECOMBINASE GP35; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; BACTERIAL DNA; VIRUS PROTEIN;

BACTERIOPHAGE; BACTERIUM; DNA; ENZYME ACTIVITY; GENETIC ENGINEERING; KINSHIP; NEW RECORD; PHYLOGENETICS; POLYMERASE CHAIN REACTION; RECOMBINATION;

ALPHA AMYLASE GENE; ARTICLE; BACILLUS SUBTILIS; BACTERIOPHAGE; CONTROLLED STUDY; ENTEROBACTERIA PHAGE LAMBDA; ESCHERICHIA COLI; GENE; GENE LOCUS; HOST; NONHUMAN; PHYLOGENETIC TREE; POLYMERASE CHAIN REACTION; PURINE NUCLEOSIDE PHOSPHORYLASE GENE; URACIL PHOSPHORIBOSYLTRANSFERASE GENE; ENZYMOLOGY; EVALUATION STUDY; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; METABOLISM; PROCEDURES;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BACILLUS SUBTILIS; BACTERIOPHAGES; DNA, BACTERIAL; DNA, SINGLE-STRANDED; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION; RECOMBINASES; RECOMBINATION, GENETIC; VIRAL PROTEINS;

EID: 84929948449     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6485-5     Document Type: Article

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