The effects on bronchial epithelial mucociliary cultures of coarse, fine, and ultrafine particulate matter from an underground railway station

Toxicological Sciences

Volumn 145, Issue 1, 2015, Pages 98-107

  Loxham, Matthew ^a,b   Morgan Walsh, Rebecca J ^a   Cooper, Matthew J ^b   Blume, Cornelia ^a   Swindle, Emily J ^a,b   Dennison, Patrick W ^a,c   Howarth, Peter H ^a,c   Cassee, Flemming R ^d,e   Teagle, Damon A H ^b   Palmer, Martin R ^b   Davies, Donna E ^a,b,c  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^c UNIVERSITY HOSPITAL SOUTHAMPTON NHS FOUNDATION TRUST   (United Kingdom)

^d NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^e UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Bronchial epithelium; Environmental exposure; metals; Particulatematter; Primary cell culture; Underground railway

Indexed keywords

ACETYLCYSTEINE; DEFEROXAMINE; HEME OXYGENASE 1; INTERLEUKIN 8; LACTATE DEHYDROGENASE; REACTIVE OXYGEN METABOLITE; PARTICULATE MATTER;

ARTICLE; CELL DIFFERENTIATION; CELL VACUOLE; CONTROLLED STUDY; CYTOSOL; ENZYME RELEASE; HUMAN; HUMAN CELL; LUNG ALVEOLUS EPITHELIUM CELL; MITOCHONDRION; PARTICULATE MATTER; PROTEIN EXPRESSION; RAILWAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSMISSION ELECTRON MICROSCOPY; BRONCHUS; CELL CULTURE; CYTOLOGY; DRUG EFFECTS; EPITHELIUM CELL; EUKARYOTIC FLAGELLUM; PARTICLE SIZE; TOXICITY; TRAFFIC AND TRANSPORT;

BRONCHI; CELLS, CULTURED; CILIA; EPITHELIAL CELLS; HUMANS; PARTICLE SIZE; PARTICULATE MATTER; TRANSPORTATION;

EID: 84929698454     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfv034     Document Type: Article

References (51)

1. Bachoual, R., Boczkowski, J., Goven, D., Amara, N., Tabet, L., On, D., Lecon-Malas, V., Aubier, M., and Lanone, S. (2007). Biological effects of particles from the Paris subway system. Chem. Res. Toxicol. 20, 1426-1433.
2. Baulig, A., Garlatti, M., Bonvallot, V., Marchand, A., Barouki, R., Marano, F., and Baeza-Squiban, A. (2003). Involvement of reactive oxygen species in the metabolic pathways triggered by diesel exhaust particles in human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 285, L671-L679.
3. Belade, E., Armand, L., Martinon, L., Kheuang, L., Fleury-Feith, J., Baeza-Squiban, A., Lanone, S., Billon-Galland, M. A., Pairon, J. C., and Boczkowski, J. (2012). A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines. Toxicol. in vitro 26, 57-66.
4. Bigert, C., Alderling, M., Svartengren, M., Plato, N., de Faire, U., and Gustavsson, P. (2008). Blood markers of inflammation and coagulation and exposure to airborne particles in employees in the Stockholm underground. Occup. Environ. Med. 65, 655-658.
5. Bigert, C., Alderling, M., Svartengren, M., Plato, N., and Gustavsson, P. (2011). No short-term respiratory effects among particle-exposed employees in the Stockholm subway. Scand. J. Work. Environ. Health 37, 129-135.
6. Bigert, C., Klerdal, K., Hammar, N., and Gustavsson, P. (2007). Myocardial infarction in Swedish subway drivers. Scand. J. Work. Environ. Health 33, 267-271.
7. Billet, S., Garcon, G., Dagher, Z., Verdin, A., Ledoux, F., Cazier, F., Courcot, D., Aboukais, A., and Shirali, P. (2007). Ambient particulate matter (PM2.5): physicochemical characterization and metabolic activation of the organic fraction in human lung epithelial cells (A549). Environ. Res. 105, 212-223.
8. Bowler, R. P. and Crapo, J. D. (2002). Oxidative stress in allergic respiratory diseases. J. Allergy Clin. Immunol. 110, 349-356.
9. Brook, R. D., Rajagopalan, S., Pope, C. A., Brook, J. R., Bhatnagar, A., Diez-Roux, A. V., Holguin, F., Hong, Y. L., Luepker, R. V., Mittleman, M. A., et al. (2010). Particulate matter air pollution and cardiovascular disease an update to the scientific statement from the American Heart Association. Circulation 121, 2331-2378.
10. Brunekreef, B. and Holgate, S. T. (2002). Air pollution and health. Lancet 360, 1233-1242.
11. Bucchieri, F., Puddicombe, S. M., Lordan, J. L., Richter, A., Buchanan, D., Wilson, S. J., Ward, J., Zummo, G., Howarth, P. H., Djukanovic, R., et al. (2002). Asthmatic bronchial epithelium is more susceptible to oxidant-induced apoptosis. Am. J. Respir. Cell Mol. Biol. 27, 179-85.
12. Calcabrini, A., Meschini, S., Marra, M., Falzano, L., Colone, M., De Berardis, B., Paoletti, L., Arancia, G., and Fiorentini, C. (2004). Fine environmental particulate engenders alterations in human lung epithelial A549 cells. Environ. Res. 95, 82-91.
13. Choi, H. S., Ashitate, Y., Lee, J. H., Kim, S. H., Matsui, A., Insin, N., Bawendi, M. G., Semmler-Behnke, M., Frangioni, J. V., and Tsuda, A. (2010). Rapid translocation of nanoparticles from the lung airspaces to the body. Nat. Biotechnol. 28, 1300-1303.
14. Derendorf, H., Hochhaus, G., Meibohm, B., Mollmann, H., and Barth, J. (1998). Pharmacokinetics and pharmacodynamics of inhaled corticosteroids. J. Allergy Clin. Immunol. 101, S440-S446.
15. Dockery, D. W., Pope, C. A., Xu, X. P., Spengler, J. D., Ware, J. H., Fay, M. E., Ferris, B. G., and Speizer, F. E. (1993). An Association between Air-Pollution and Mortality in 6 United States Cities. N. Engl. J. Med. 329, 1753-1759.
16. Freyre-Fonseca, V., Delgado-Buenrostro, N. L., Gutierrez-Cirlos, E. B., Calderon-Torres, C. M., Cabellos-Avelar, T., Sanchez-Perez, Y., Pinzon, E., Torres, I., Molina-Jijon, E., Zazueta, C., et al. (2011). Titanium dioxide nanoparticles impair lung mitochondrial function. Toxicol. Lett. 202, 111-119.
17. Geiser, M., Rothen-Rutishauser, B., Kapp, N., Schurch, S., Kreyling, W., Schulz, H., Semmler, M., Hof, V. I., Heyder, J., and Gehr, P. (2005). Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells. Environ. Health Perspect. 113, 1555-1560.
18. Gustavsson, P., Bigert, C., and Pollan, M. (2008). Incidence of lung cancer among subway drivers in Stockholm. Am. J. Ind. Med. 51, 545-547.
19. Jachak, A., Lai, S. K., Hida, K., Suk, J. S., Markovic, N., Biswal, S., Breysse, P. N., and Hanes, J. (2012). Transport of metal oxide nanoparticles and single-walled carbon nanotubes in human mucus. Nanotoxicology 6, 614-622.
20. Janssen, N. A. H., Brunekreef, B., van Vliet, P., Aarts, F., Meliefste, K., Harssema, H., and Fischer, P. (2003). The relationship between air pollution from heavy traffic and allergic sensitization, bronchial hyperresponsiveness, and respiratory symptoms in Dutch schoolchildren. Environ. Health Perspect. 111, 1512-1518.
21. Johnson, M. (1998). Development of fluticasone propionate and comparison with other inhaled corticosteroids. J. Allergy Clin. Immunol. 101, S434-S439.
22. Karlsson, H. L., Holgersson, A., and Moller, L. (2008). Mechanisms related to the genotoxicity of particles in the subway and from other sources. Chem. Res. Toxicol. 21, 726-731.
23. Karlsson, M., Kurz, T., Brunk, U. T., Nilsson, S. E., and Frennesson, C. I. (2010). What does the commonly used DCF test for oxidative stress really show? Biochem. J. 428, 183-190.
24. Karlsson, H. L., Ljungman, A. G., Lindbom, J., and Moller, L. (2006). Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway. Toxicol. Lett. 165, 203-211.
25. Kim, S., Shen, S., Sioutas, C., Zhu, Y. F., and Hinds, W. C. (2002). Size distribution and diurnal and seasonal trends of ultrafine particles in source and receptor sites of the Los Angeles basin. J. Air Waste Manag. Assoc. 52, 297-307.
26. Klepczynska-Nystrom, A., Larsson, B. M., Grunewald, J., Pousette, C., Lundin, A., Eklund, A., and Svartengren, M. (2012). Health effects of a subway environment in mild asthmatic volunteers. Respir. Med. 106, 25-33.
27. Konczol, M., Ebeling, S., Goldenberg, E., Treude, F., Gminski, R., Giere, R., Grobety, B., Rothen-Rutishauser, B., Merfort, I., and Mersch-Sundermann, V. (2011). Cytotoxicity and genotoxicity of size-fractionated iron oxide (magnetite) in A549 human lung epithelial cells: role of ROS, JNK, and NF-kappa B. Chem. Res. Toxicol. 24, 1460-1475.
28. Leino, M. S., Loxham, M., Blume, C., Swindle, E. J., Jayasekera, N. P., Dennison, P. W., Shamji, B. W., Edwards, M. J., Holgate, S. T., Howarth, P. H., et al. (2013). Barrier disrupting effects of Alternaria alternata extract on bronchial epithelium from asthmatic donors. PLoS One 8, e71278.
29. Li, N., Alam, J., Venkatesan, M. I., Eiguren-Fernandez, A., Schmitz, D., Di Stefano, E., Slaughter, N., Killeen, E., Wang, X. R., Huang, A., et al. (2004). Nrf2 is a key transcription factor that regulates antioxidant defense in macrophages and epithelial cells: protecting against the proinflammatory and oxidizing effects of diesel exhaust chemicals. J. Immunol. 173, 3467-3481.
30. Li, N., Sioutas, C., Cho, A., Schmitz, D., Misra, C., Sempf, J., Wang, M. Y., Oberley, T., Froines, J., and Nel, A. (2003). Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage. Environ. Health Perspect. 111, 455-460.
31. Lim, S. S., Vos, T., Flaxman, A. D., Danaei, G., Shibuya, K., Adair-Rohani, H., Amann, M., Anderson, H. R., Andrews, K. G., Aryee, M., et al. (2012). A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 380, 2224-2260.
32. Lindbom, J., Gustafsson, M., Blomqvist, G., Dahl, A., Gudmundsson, A., Swietlicki, E., and Ljungman, A. G. (2006). Exposure to wear particles generated from studded tires and pavement induces inflammatory cytokine release from human macrophages. Chem. Res. Toxicol. 19, 521-530.
33. Logan, W. P. (1953). Mortality in the London fog incident, 1952. Lancet 1, 336-8.
34. Lordan, J. L., Bucchieri, F., Richter, A., Konstantinidis, A., Holloway, J. W., Thornber, M., Puddicombe, S. M., Buchanan, D., Wilson, S. J., Djukanovic, R., et al. (2002). Cooperative effects of Th2 cytokines and allergen on normal and asthmatic bronchial epithelial cells. J. Immunol. 169, 407-414.
35. Loxham, M., Cooper, M. J., Gerlofs-Nijland, M. E., Cassee, F. R., Davies, D. E., Palmer, M. R., and Teagle, D. A. (2013). Physicochemical characterization of airborne particulate matter at a mainline underground railway station. Environ. Sci. Technol. 47, 3614-22.
36. Loxham, M., Davies, D. E., and Blume, C. (2014). Epithelial function and dysfunction in asthma. Clin. Exp. Allergy 44, 1299-1313.
37. Maynard, R. L. (2013). A new analogy for the effects of ambient particles on health. Part. Fibre Toxicol. 10, 30.
38. Montefort, S., Roberts, J. A., Beasley, R., Holgate, S. T., and Roche, W. R. (1992). The site of disruption of the bronchial epithelium in asthmatic and nonasthmatic subjects. Thorax 47, 499-503.
39. Phalen, R. F., Oldham, M. J., and Nel, A. E. (2006). Tracheobronchial particle dose considerations for in vitro toxicology studies. Toxicol. Sci. 92, 126-132.
40. Pope, C. A. (1989). Respiratory-disease associated with community air-pollution and a steel mill, Utah Valley. Am. J. Public Health 79, 623-628.
41. Querol, X., Moreno, T., Karanasiou, A., Reche, C., Alastuey, A., Viana, M., Font, O., Gil, J., de Miguel, E., and Capdevila, M. (2012). Variability of levels and composition of PM10 and PM2.5 in the Barcelona metro system. Atmos. Chem. Phys. 12, 5055-5076.
42. Robinson, J. P., Bruner, L. H., Bassoe, C. F., Hudson, J. L., Ward, P. A., and Phan, S. H. (1988). Measurement of intracellular fluorescence of human-monocytes relative to oxidativemetabolism. J. Leukoc. Biol. 43, 304-310.
43. Royall, J. A. and Ischiropoulos, H. (1993). Evaluation of 2',7'- dichlorofluorescin and dihydrorhodamine 123 as fluorescent- probes for intracellular H2O2 in cultured endothelialcells. Arch. Biochem. Biophys. 302, 348-355.
44. Schaudien, D., Knebel, J., and Creutzenberg, O. (2012). In vitro study revealed different size behavior of different nanoparticles. J. Nanopart. Res. 14, 1039.
45. Seaton, A., Cherrie, J., Dennekamp, M., Donaldson, K., Hurley, J. F., and Tran, C. L. (2005). The London Underground: dust and hazards to health. Occup. Environ. Med. 62, 355-362.
46. Steenhof, M., Mudway, I. S., Gosens, I., Hoek, G., Godri, K. J., Kelly, F. J., Harrison, R. M., Pieters, R. H. H., Cassee, F. R., Lebret, E., et al. (2013). Acute nasal pro-inflammatory response to air pollution depends on characteristics other than particle mass concentration or oxidative potential: the RAPTES project. Occup. Environ. Med. 70, 341-348.
47. Strak, M., Steenhof, M., Godri, K. J., Gosens, I., Mudway, I. S., Cassee, F. R., Lebret, E., Brunekreef, B., Kelly, F. J., Harrison, R. M., et al. (2011). Variation in characteristics of ambient particulate matter at eight locations in the Netherlands-the RAPTES project. Atmos. Environ. 45, 4442-4453.
48. Swift, L. M. and Sarvazyan, N. (2000). Localization of dichlorofluorescin in cardiac myocytes: implications for assessment of oxidative stress. Am J Physiol. Heart C 278, H982-H990.
49. Upadhyay, D., Panduri, V., Ghio, A., and Kamp, D. W. (2003). Particulate matter induces alveolar epithelial cell DNA damage and apoptosis-role of free radicals and the mitochondria. Am. J. Respir. Cell Mol. Biol. 29, 180-187.
50. van Klaveren, R. J. and Nemery, B. (1999). Role of reactive oxygen species in occupational and environmental obstructive pulmonary diseases. Curr. Opin. Pulm. Med. 5, 118-23.
51. Xiao, G. G., Wang, M. Y., Li, N., Loo, J. A., and Nel, A. E. (2003). Use of proteomics to demonstrate a hierarchical oxidative stress response to diesel exhaust particle chemicals in a macrophage cell line. J. Biol. Chem. 278, 50781-50790.