X-ray and Cryo-EM structures reveal mutual conformational changes of Kinesin and GTP-state microtubules upon binding

EMBO Journal

Volumn 34, Issue 9, 2015, Pages 1270-1286

  Morikawa, Manatsu ^a,b   Yajima, Hiroaki ^a,b   Nitta, Ryo ^a,b,e   Inoue, Shigeyuki ^a,b   Ogura, Toshihiko ^c   Sato, Chikara ^c   Hirokawa, Nobutaka ^a,b,d  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^e RIKEN   (Japan)

Author keywords

cryo electron microscopy; kinesin; microtubule; polarized transport in neuron; X ray crystallography

Indexed keywords

ADENOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; KINESIN; TUBULIN; 5'-GUANYLYLMETHYLENEBISPHOSPHONATE; ADENOSINE DIPHOSPHATE; KIF5C PROTEIN, MOUSE;

ARTICLE; BINDING AFFINITY; COMPUTER MODEL; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HANGING DROP VAPOR DIFFUSION METHOD; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MICROTUBULE; NERVE FIBER TRANSPORT; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY; X RAY DIFFRACTION; AMINO ACID SEQUENCE; ANALOGS AND DERIVATIVES; ANIMAL; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR DOCKING; MOLECULAR GENETICS; MOUSE; PROCEDURES; PROTEIN CONFORMATION; ULTRASTRUCTURE;

ADENOSINE DIPHOSPHATE; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CRYOELECTRON MICROSCOPY; CRYSTALLOGRAPHY, X-RAY; GUANOSINE TRIPHOSPHATE; KINESIN; MICE; MICROTUBULES; MODELS, MOLECULAR; MOLECULAR DOCKING SIMULATION; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION;

EID: 84929658259     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.15252/embj.201490588     Document Type: Article

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