Continued progress in developing the Pig-a gene mutation assay

Mutagenesis

Volumn 30, Issue 3, 2015, Pages 313-314

  Heflich, Robert H ^a   Dobrovolsky, Vasily N ^a   Dertinger, Stephen D ^b  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

^b LITRON LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCAN; PHOSPHATIDYLINOSITOL GLYCAN CLASS A; UNCLASSIFIED DRUG; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; MEMBRANE PROTEIN; PHOSPHATIDYLINOSITOL GLYCAN-CLASS A PROTEIN;

ERYTHROCYTE; GENE SILENCING; IN VIVO STUDY; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RETICULOCYTE; REVIEW; RISK ASSESSMENT; X CHROMOSOME; ANIMAL; BIOSYNTHESIS; GENETICS; HUMAN; MUTATION; REPORTER GENE;

SUIDAE;

ANIMALS; GENES, REPORTER; GPI-LINKED PROTEINS; HUMANS; MEMBRANE PROTEINS; MUTAGENESIS; MUTATION;

EID: 84929579262     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/geu082     Document Type: Review

References (15)

1. Iida, Y., Takeda, J., Miyata, T., Inoue, N., Nishimura, J., Kitani, T., Maeda, K. and Kinoshita, T. (1994) Characterization of genomic PIG-A gene: A gene for glycosylphosphatidylinositol-anchor biosynthesis and paroxysmal nocturnal hemoglobinuria. Blood, 83, 3126-3131.
2. Kawagoe, K., Takeda, J., Endo, Y. and Kinoshita, T. (1994) Molecular cloning of murine Pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus. Genomics, 23, 566-574.
3. Araten, D. J., Nafa, K., Pakdeesuwan, K. and Luzzatto, L. (1999) Clonal populations of hematopoietic cells with paroxysmal nocturnal hemoglobinuria genotype and phenotype are present in normal individuals. Proc. Natl. Acad. Sci. U. S. A., 96, 5209-5214.
4. Bryce, S. M., Bemis, J. C. and Dertinger, S. D. (2008) In vivo mutation assay based on the endogenous Pig-A locus. Environ. Mol. Mutagen., 49, 256-264.
5. Miura, D., Dobrovolsky, V. N., Kasahara, Y., Katsuura, Y. and Heflich, R. H. (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat. Environ. Mol. Mutagen., 49, 614-621.
6. Kimoto, T., Chikura, S., Suzuki, K. et al. (2011) Further development of the rat Pig-A mutation assay: measuring rat Pig-A mutant bone marrow erythroids and a high throughput assay for mutant peripheral blood reticulocytes. Environ. Mol. Mutagen., 52, 774-783.
7. Dertinger, S. D., Bryce, S. M., Phonethepswath, S. and Avlasevich, S. L. (2011) When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-A mutant frequency by flow cytometric analysis. Mutat. Res., 721, 163-170.
8. Dertinger, S. D., Phonethepswath, S., Weller, P. et al. (2011) Interlaboratory Pig-A gene mutation assay trial: Studies of 1,3-propane sultone with immunomagnetic enrichment of mutant erythrocytes. Environ. Mol. Mutagen., 52, 748-755.
9. Gollapudi, B. B., Lynch, A. M., Heflich, R. H. et al. (2015) The in vivo Pig-A assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup. Mutat. Res., in press. doi:10.1016/j.mrgentox. 2014.09.007
10. Revollo, J., Pearce, M. G., Petibone, D. M., Mittelstaedt, R. A. and Dobrovolsky, V. N. (2015) Confirmation of Pig-A mutation in flow cytrometryidentified CD48-deficient T-lymphocytes from F344 rats. Mutagenesis, 30, 315-324.
11. Kenyon, M. O., Coffing, S. L., Ackerman, J. I., Gunther, W., Dertinger, S. and Dobo, K. L. (2015) Compensatory erythropoiesis has no impact on the outcome of the in vivo Pig-A mutation assay in rats following treatment with the hemolytic agent 2-butoxyethanol. Mutagenesis, 30, 325-334.
12. Stankowski, L. F., Jr, Aardema, M. J., Lawlor, T. E., Pant, K., Roy, S., Xu, Y. and Elbekai, R. (2015) Integration of Pig-a, micronucleus, chromosome aberration, and comet assay endpoints in a 28-day rodent toxicity study with urethane. Mutagenesis, 30, 335-342.
13. Bemis, J. C., Labash, C., Avlasevich, S. L., Carlson, K., Berg, A., Torous, D. K., Barragato, M., MacGregor, J. T. and Dertinger, S. D. (2015) Rat Pig-A mutation assay responds to the genotoxic carcinogen ethyl carbamate but not the non-genotoxic carcinogen methyl carbamate. Mutagenesis, 30, 343-347.
14. Labash, C., Avlasevich, S. L., Carlson, K., Torous, D. K., Berg, A., Bemis, J. C., MacGregor, J. T. and Dertinger, S. D. (2015) Comparison of male versus female responses in the Pig-A mutation assay. Mutagenesis, 30, 349-357.
15. Godin-Ethier, J., Leroux, F., Wang, N., Thébaud, S., Merah, F. and Nelson, A. (2015) Characterization of an in vivo Pig-A mutation assay for use in regulatory toxicology studies. Mutagenesis, 30, 359-363.