Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats

Mutagenesis

Volumn 30, Issue 3, 2015, Pages 315-324

  Revollo, Javier ^a   Pearce, Mason G ^a   Petibone, Dayton M ^a   Mittelstaedt, Roberta A ^a   Dobrovolsky, Vasily N ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD48 ANTIGEN; ETHYLNITROSOUREA; GENOMIC DNA; GLYCOSYLPHOSPHATIDYLINOSITOL; LEUKOCYTE ANTIGEN; MEMBRANE PROTEIN; MUTAGENIC AGENT; PHOSPHATIDYLINOSITOL GLYCAN-CLASS A PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOASSAY; CELL SELECTION; CONTROLLED STUDY; ERYTHROCYTE; FISCHER 344 RAT; FLOW CYTOMETRY; GENE; GENE MUTATION; GENOME ANALYSIS; IN VIVO STUDY; INDUCED MUTATION; LYMPHOCYTE; LYMPHOCYTE CLONE; MALE; MARKER GENE; MOLECULAR BIOLOGY; NEXT GENERATION SEQUENCING; NONHUMAN; PIG A GENE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT; SPLEEN LYMPHOCYTE; T LYMPHOCYTE; ANIMAL; CELL CULTURE; DNA MUTATIONAL ANALYSIS; GENETICS; HIGH THROUGHPUT SEQUENCING; IMMUNOMAGNETIC SEPARATION; METABOLISM; MUTAGEN TESTING; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; PHENOTYPE;

ANIMALIA; RATTUS; SUIDAE;

ANIMALS; ANTIGENS, CD; BASE SEQUENCE; CELLS, CULTURED; DNA MUTATIONAL ANALYSIS; ETHYLNITROSOUREA; FLOW CYTOMETRY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; IMMUNOMAGNETIC SEPARATION; MALE; MEMBRANE PROTEINS; MUTAGENESIS; MUTAGENICITY TESTS; MUTAGENS; MUTATION; PHENOTYPE; RATS, INBRED F344; T-LYMPHOCYTES;

EID: 84929583420     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/geu030     Document Type: Article

References (17)

1. Dobrovolsky, V. N., Miura, D., Heflich, R. H. and Dertinger, S. D. (2010) The in vivo Pig-A gene mutation assay, a potential tool for regulatory safety assessment. Environ. Mol. Mutagen., 51, 825-835.
2. Luzzatto, L. (2006) Paroxysmal nocturnal hemoglobinuria: an acquired X-linked genetic disease with somatic-cell mosaicism. Curr. Opin. Genet. Dev., 16, 317-322.
3. Nishimura, J., Murakami, Y. and Kinoshita, T. (1999) Paroxysmal nocturnal hemoglobinuria: An acquired genetic disease. Am. J. Hematol., 62, 175-182.
4. Kinoshita, T., Fujita, M. and Maeda, Y. (2008) Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress. J. Biochem., 144, 287-294.
5. Tiede, A., Bastisch, I., Schubert, J., Orlean, P. and Schmidt, R. E. (1999) Biosynthesis of glycosylphosphatidylinositols in mammals and unicellular microbes. Biol. Chem., 380, 503-523.
6. Richards, S. J. and Barnett, D. (2007) The role of flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria in the clinical laboratory. Clin. Lab. Med., 27, 577-90.
7. Dertinger, S. D., Phonethepswath, S., Weller, P., et al. (2011) International Pig-A gene mutation assay trial: evaluation of transferability across 14 laboratories. Environ. Mol. Mutagen., 52, 690-698.
8. Dertinger, S. D., Phonethepswath, S., Avlasevich, S. L., et al. (2012) Efficient monitoring of in vivo pig-A gene mutation and chromosomal damage: summary of 7 published studies and results from 11 new reference compounds. Toxicol. Sci., 130, 328-348.
9. Miura, D., Dobrovolsky, V. N., Mittelstaedt, R. A., Kasahara, Y., Katsuura, Y. and Heflich, R. H. (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants. Environ. Mol. Mutagen., 49, 622-630.
10. Miura, D., Shaddock, J. G., Mittelstaedt, R. A., Dobrovolsky, V. N., Kimoto, T., Kasahara, Y. and Heflich, R. H. (2011) Analysis of mutations in the Pig-A gene of spleen T-cells from N-ethyl-N-nitrosourea-treated fisher 344 rats. Environ. Mol. Mutagen., 52, 419-423.
11. Kimoto, T., Suzuki, K., Kobayashi, X. M., Dobrovolsky, V. N., Heflich, R. H., Miura, D. and Kasahara, Y. (2011) Manifestation of Pig-A mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: direct sequencing of Pig-A cDNA from bone marrow cells negative for GPI-anchored protein expression. Mutat. Res., 723, 36-42.
12. Dobrovolsky, V. N., Boctor, S. Y., Twaddle, N. C., et al. (2010) Flow cytometric detection of Pig-A mutant red blood cells using an erythroid-specific antibody: application of the method for evaluating the in vivo genotoxicity of methylphenidate in adolescent rats. Environ. Mol. Mutagen., 51, 138-145.
13. Dobrovolsky, V. N., Shaddock, J. G., Mittelstaedt, R. A., Miura, D. and Heflich, R. H. (2013) Detection of in vivo mutation in the Hprt and Pig-A genes of rat lymphocytes. Methods Mol. Biol., 1044, 79-95.
14. Mittelstaedt, R. A., Smith, B. A. and Heflich, R. H. (1995) Analysis of in vivo mutation induced by N-ethyl-N-nitrosourea in the hprt gene of rat lymphocytes. Environ. Mol. Mutagen., 26, 261-269.
15. Gilles, A., Meglécz, E., Pech, N., Ferreira, S., Malausa, T. and Martin, J. F. (2011) Accuracy and quality assessment of 454 GS-FLX Titanium pyrosequencing. BMC Genomics, 12, 245.
16. Dertinger, S. D., Bryce, S. M., Phonethepswath, S. and Avlasevich, S. L. (2011) When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-A mutant frequency by flow cytometric analysis. Mutat. Res., 721, 163-170.
17. Shane, B. S., de Boer, J., Watson, D. E., Haseman, J. K., Glickman, B. W. and Tindall, K. R. (2000) LacI mutation spectra following benzo[a]pyrene treatment of Big Blue mice. Carcinogenesis, 21, 715-725.