Comparison of male versus female responses in the Pig-a mutation assay

Mutagenesis

Volumn 30, Issue 3, 2015, Pages 349-357

  Labash, Carson ^a   Avlasevich, Svetlana L ^a   Carlson, Kristine ^a   Torous, Dorothea K ^a   Berg, Ariel ^a   Bemis, Jeffrey C ^a   Macgregor, James T ^b   Dertinger, Stephen D ^a  

^a LITRON LABORATORIES   (United States)

^b Toxicology Consulting Services   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ETHYLNITROSOUREA; PIG A PROTEIN; PROTEIN; UNCLASSIFIED DRUG; MEMBRANE PROTEIN; MUTAGENIC AGENT; PHOSPHATIDYLINOSITOL GLYCAN-CLASS A PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; ERYTHROCYTE; FEMALE; FLOW CYTOMETRY; GENE MUTATION; IMMUNOMAGNETIC SEPARATION; MALE; NONHUMAN; PHENOTYPE; PIG A GENE MUTATION ASSAY; PRIORITY JOURNAL; RAT; RETICULOCYTE; SEX DIFFERENCE; ANIMAL; COMPARATIVE STUDY; DRUG EFFECTS; GENETICS; MICRONUCLEUS TEST; MUTAGENESIS; MUTATION; MUTATION RATE; SPRAGUE DAWLEY RAT;

RATTUS; RODENTIA; SUIDAE;

ANIMALS; ETHYLNITROSOUREA; FEMALE; MALE; MEMBRANE PROTEINS; MICRONUCLEUS TESTS; MUTAGENESIS; MUTAGENS; MUTATION; MUTATION RATE; RATS, SPRAGUE-DAWLEY; RETICULOCYTES;

EID: 84929577223     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/geu055     Document Type: Article

References (27)

1. Araten, D. J., Nafa, K., Pakdeesuwan, K. and Luzzatto, L. (1999) Clonal populations of hematopoietic cells with paroxysmal nocturnal hemoglobinuria genotype and phenotype are present in normal individuals. Proc. Natl. Acad. Sci. U. S. A., 96, 5209-5214.
2. Chen, R., Eshleman, J. R., Brodsky, R. A. and Medof, M. E. (2001) Glycophosphatidylinositol-anchored protein deficiency as a marker of mutator phenotypes in cancer. Cancer Res., 61, 654-658.
3. Bryce, S. M., Bemis, J. C. and Dertinger, S. D. (2008) In vivo mutation assay based on the endogenous Pig-A locus. Environ. Mol. Mutagen., 49, 256-264.
4. Miura, D., Dobrovolsky, V. N., Kasahara, Y., Katsuura, Y. and Heflich, R. H. (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat. Environ. Mol. Mutagen., 49, 614-621.
5. Dobrovolsky, V. N., Miura, D., Heflich, R. H. and Dertinger, S. D. (2010) The in vivo Pig-A gene mutation assay, a potential tool for regulatory safety assessment. Environ. Mol. Mutagen., 51, 825-835.
6. Dertinger, S.D., Phonethepswath, S., Franklin, D., et al. (2010) Integration of mutation and chromosomal damage endpoints into 28-day repeat dose toxicology studies, Toxicol. Sci., 115, 401-411.
7. Stankowski, L. F. Jr, Roberts, D. J., Chen, H., Lawlor, T., McKeon, M., Murli, H., Thakur, A. and Xu, Y. (2011) Integration of Pig-a, micronucleus, chromosome aberration, and Comet assay endpoints in a 28-day rodent toxicity study with 4-nitroquinoline-1-oxide. Environ. Mol. Mutagen., 52, 738-747.
8. Lynch, A. M., Giddings, A., Custer, L., Gleason, C., Henwood, A., Aylott, M. and Kenny, J. (2011) International Pig-A gene mutation assay trial (stage III): results with N-methyl-N-nitrosourea. Environ. Mol. Mutagen., 52, 699-710.
9. Lynch, A. M., Sasaki, J. C., Elespuru, R., et al. (2011) New and emerging technologies for genetic toxicity testing. Environ. Mol. Mutagen., 52, 205-223.
10. Schuler, M., Gollapudi, B. B., Thybaud, V. and Kim, J. H. (2011) Need and potential value of the Pig-ain vivo mutation assay-A HESI perspective. Environ. Mol. Mutagen., 52, 685-689.
11. Dertinger, S.D., Phonethepswath, S., Weller, P. et al. (2011) International Pig-A gene mutation assay trial: Evaluation of transferability across fourteen laboratories. Environ. Mol. Mutagen., 52, 690-698.
12. Kimoto, T., Horibata, K., Chikura, S. et al. (2013) Interlaboratory trial of the rat Pig-A mutation assay using an erythroid marker HIS49 antibody. Mutat. Res., 755, 126-134.
13. Bhalli, J. A., Shaddock, J. G., Pearce, M. G., Dobrovolsky, V. N., Cao, X., Heflich, R. H. and Vohr, H. W. (2011) Report on stage III Pig-A mutation assays using benzo[a]pyrene. Environ. Mol. Mutagen., 52, 731-737.
14. Cammerer, Z., Bhalli, J.A., Cao, X. et al. (2011) Report on Stage III Pig-A mutation assays using N-ethyl-N-nitrosourea-Comparison with other in vivo genotoxicity endpoints. Environ. Mol. Mutagen., 52, 721-730.
15. Gollapudi, B.B., Lynch, A.M., Heflich, R.H. et al. (2014) The in vivo pig-A assay: A report of the International Workshop on Genotoxicity Testing (IWGT) Workgroup. Mutat. Res., in press, doi:10.1016/j.mrgentox. 2014.09.007.
16. Torous, D. K., Hall, N. E., Murante, F. G., Gleason, S. E., Tometsko, C. R. and Dertinger, S. D. (2003) Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy. Toxicol. Sci., 74, 309-314.
17. Dertinger, S. D., Phonethepswath, S., Avlasevich, S. L., Torous, D. K., Mereness, J., Cottom, J., Bemis, J. C. and Macgregor, J. T. (2014) Pig-A gene mutation and micronucleated reticulocyte induction in rats exposed to tumorigenic doses of the leukemogenic agents chlorambucil, thiotepa, melphalan, and 1,3-propane sultone. Environ. Mol. Mutagen., 55, 299-308.
18. Vardeman, S.B. (1992). What about other intervals? The American Statistician. 46, 193-197.
19. Dertinger, S.D., Camphausen, K., MacGregor, J.T. et al. (2004) Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood. Environ. Mol. Mutagen., 44, 427-435.
20. Tometsko, A. M., Torous, D. K. and Dertinger, S. D. (1993) Analysis of micronucleated cells by flow cytometry. 1. Achieving high resolution with a malaria model. Mutat. Res., 292, 129-135.
21. Dertinger, S. D., Bryce, S. M., Phonethepswath, S. and Avlasevich, S. L. (2011) When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-A mutant frequency by flow cytometric analysis. Mutat. Res., 721, 163-170.
22. Dertinger, S.D., Phonethepswath, S., Weller, P. et al. (2011c) Interlaboratory Pig-A gene mutation assay trial: Studies of 1,3-propane sultone with immunomagnetic enrichment of mutant erythrocytes. Environ. Mol. Mutagen., 52, 748-755.
23. Dertinger, S.D., Phonethepswath, S., Avlasevich, S.L., Torous, D.K., Mereness, J., Bryce, S.M., Bemis, J.C., Bell, S., Weller, P. and MacGregor, J.T. (2012) Efficient monitoring of in vivo Pig-A gene mutation and chromosomal damage: Summary of 7 published studies and results from 11 new reference compounds. Toxicol. Sci., 130, 328-348.
24. Phonethepswath, S., Franklin, D., Torous, D.K. et al. (2010) Pig-A mutation: Kinetics in rat erythrocytes following exposure to five prototypical mutagens. Toxicol. Sci., 114, 59-70.
25. OECD (1997) Guideline for the testing of chemicals. Mammalian erythrocyte micronucleus test, Guideline 474.
26. Clayton, J.A. and Collins, F.S. (2014) NIH to balance sex in cell and animal studies. Nature, 509, 282-283.
27. Kenyon, M., Coffing, S., Ackerman, J., Gunther, W., Dertinger, S.D., Criswell, K., Dobo, K. (2015) Compensatory erythropoiesis has no impact on the outcome of the in vivo Pig-A mutation assay in rats following treatment with the hemolytic agent 2-butoxyethanol. Mutagenesis, 30, 325-334.