Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Caires, Hugo R ^a,b,c   Gomez Lazaro, Maria ^a,b   Oliveira, Carla M ^a,b,c   Gomes, David ^a,b   Mateus, Denisa D ^a,c   Oliveira, Carla ^a,c,d   Barrias, Cristina C ^a,b   Barbosa, Mário A ^a,b,c   Almeida, Catarina R ^a,b  

^a UNIVERSITY OF PORTO   (Portugal)

^b INEB INSTITUTO DE ENGENHARIA BIOMÉDICA   (Portugal)

^c UNIVERSITY OF PORTO   (Portugal)

^d University of Porto   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; BIOMATERIAL; DENDRA2 PROTEIN, DENDRONEPHTHYA; PHOTOPROTEIN;

BIOENGINEERING; BONE MARROW CELL; CELL CULTURE; CELL MOTION; CELL SURVIVAL; CELL TRACKING; CYTOLOGY; GENETIC TRANSFECTION; GENETICS; HUMAN; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; PHYSIOLOGY; PROCEDURES; THREE DIMENSIONAL IMAGING; TISSUE SCAFFOLD; WOUND HEALING;

BIOCOMPATIBLE MATERIALS; BIOENGINEERING; BIOMARKERS; BONE MARROW CELLS; CELL MOVEMENT; CELL SURVIVAL; CELL TRACKING; CELLS, CULTURED; HUMANS; IMAGING, THREE-DIMENSIONAL; LUMINESCENT PROTEINS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; TISSUE SCAFFOLDS; TRANSFECTION; WOUND HEALING;

EID: 84929376876     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep10079     Document Type: Article

References (50)

1. Bartunek J., et al. Cardiopoietic stem cell therapy in heart failure: the C-CURE (Cardiopoietic stem Cell therapy in heart failURE) multicenter randomized trial with lineage-specified biologics. J. Am. Coll. Cardiol. 61, 2329-2338 (2013).
2. Elliott M. J., et al. Stem-cell-based, tissue engineered tracheal replacement in a child: a 2-year follow-up study. Lancet 380, 994-1000 (2012).
3. Shekaran A., et al. Bone regeneration using an alpha 2 beta 1 integrin-specific hydrogel as a BMP-2 delivery vehicle. Biomaterials 35, 5453-5461 (2014).
4. Goncalves R. M., Antunes J. C., Barbosa M. A. Mesenchymal stem cell recruitment by stromal derived factor-1-delivery systems based on chitosan/poly(gamma-glutamic acid) polyelectrolyte complexes. Eur. Cell. Mater. 23, 249-260; discussion 260-241 (2012).
5. Guo Y., et al. Assessment of the green florescence protein labeling method for tracking implanted mesenchymal stem cells. Cytotechnology 64, 391-401 (2012).
6. Polzer H., et al. Long-term detection of fluorescently labeled human mesenchymal stem cell in vitro and in vivo by semi-automated microscopy. Tissue Eng. Part C Methods 18, 156-165 (2012).
7. Taghizadeh R. R., Sherley J. L. CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells. J. Biomed. Biotechnol. 2008, 453590 (2008).
8. Lalande C., et al. Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering. Eur. Cell. Mater. 21, 341-354 (2011).
9. Bago J. R., et al. In vivo bioluminescence imaging of cell differentiation in biomaterials: a platform for scaffold development. Tissue Eng. Part A 19, 593-603 (2013).
10. Granero-Molto F., et al. Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells 27, 1887-1898 (2009).
11. Teo G. S., Yang Z., Carman C. V., Karp J. M., Lin C. P. Intravital Imaging of Mesenchymal Stem Cell Trafficking and Association with Platelets and Neutrophils. Stem Cells, (2014).
12. Muller-Borer B. J., Collins M. C., Gunst P. R., Cascio W. E., Kypson A. P. Quantum dot labeling of mesenchymal stem cells. J. Nanobiotechnology 5, 9 (2007).
13. Collins M. C., Gunst P. R., Cascio W. E., Kypson A. P., Muller-Borer B. J. Labeling and imaging mesenchymal stem cells with quantum dots. Methods Mol. Biol. 906, 199-210 (2012).
14. Gildehaus F. J., et al. Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo. Mol. Imaging. Biol. 13, 1204-1214 (2011).
15. Ji F., Duan H. G., Zheng C. Q., Li J. Comparison of chloromethyl-dialkylcarbocyanine and green fluorescent protein for labeling human umbilical mesenchymal stem cells. Biotechnol. Lett., (2014).
16. Bins A. D., et al. Intravital imaging of fluorescent markers and FRET probes by DNA tattooing. BMC Biotechnol. 7, 2 (2007).
17. Chernet B. T., Adams D. S., Levin M. Photoconversion for tracking the dynamics of cell movement in Xenopus laevis embryos. Cold Spring Harb. Protoc. 2012, 683-690 (2012).
18. Nowotschin S., Hadjantonakis A. K. Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos. BMC Dev. Biol. 9, 49 (2009).
19. Baker S. M., Buckheit R. W., 3rd, Falk M. M. Green-to-red photoconvertible fluorescent proteins: tracking cell and protein dynamics on standard wide-field mercury arc-based microscopes. BMC Cell. Biol. 11, 15 (2010).
20. Kedrin D., et al. Intravital imaging of metastatic behavior through a mammary imaging window. Nat. Methods 5, 1019-1021 (2008).
21. Chudakov D. M., Lukyanov S., Lukyanov K. A. Using photoactivatable fluorescent protein Dendra2 to track protein movement. Biotechniques 42, 553, 555, 557 passim (2007).
22. Chudakov D. M., Lukyanov S., Lukyanov K. A. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. Nat. Protoc. 2, 2024-2032 (2007).
23. Griswold S. L., Sajja K. C., Jang C. W., Behringer R. R. Generation and characterization of iUBC-KikGR photoconvertible transgenic mice for live time-lapse imaging during development. Genesis 49, 591-598 (2011).
24. Carlson A. L., et al. Correction: Tracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Label. PLoS One 8, (2013).
25. Wu S., Koizumi K., Macrae-Crerar A., Gallagher K. L. Assessing the utility of photoswitchable fluorescent proteins for tracking intercellular protein movement in the Arabidopsis root. PLoS One 6, e27536 (2011).
26. Zaman M. H., et al. Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. Proc. Natl. Acad. Sci. USA 103, 10889-10894 (2006).
27. Fonseca K. B., et al. Injectable MMP-sensitive alginate hydrogels as hMSC delivery systems. Biomacromolecules 15, 380-390 (2014).
28. Bidarra S. J., Barrias C. C., Fonseca K. B., Barbosa M. A., Soares R. A., Granja P. L. Injectable in situ crosslinkable RGD-modified alginate matrix for endothelial cells delivery. Biomaterials 32, 7897-7904 (2011).
29. Ghibaudo M., Di Meglio J. M., Hersen P., Ladoux B. Mechanics of cell spreading within 3D-micropatterned environments. Lab. Chip. 11, 805-812 (2011).
30. Discher D. E., Janmey P., Wang Y. L. Tissue cells feel and respond to the stiffness of their substrate. Science 310, 1139-1143 (2005).
31. Ridley A. J., et al. Cell migration: integrating signals from front to back. Science 302, 1704-1709 (2003).
32. Maia F. R., Fonseca K. B., Rodrigues G., Granja P. L., Barrias C. C. Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels. Acta Biomater. 10, 3197-3208 (2014).
33. Santos S. G., et al. Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response. Acta Biomater. 9, 7209-7217 (2013).
34. Barbosa J. N., Amaral I. F., Aguas A. P., Barbosa M. A. Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds. J. Biomed. Mater. Res. A 93, 20-28 (2010).
35. Helledie T., Nurcombe V., Cool S. M. A simple and reliable electroporation method for human bone marrow mesenchymal stem cells. Stem Cells Dev. 17, 837-848 (2008).
36. Bidarra S. J., Barrias C. C., Barbosa M. A., Soares R., Granja P. L. Immobilization of human mesenchymal stem cells within RGD-grafted alginate microspheres and assessment of their angiogenic potential. Biomacromolecules 11, 1956-1964 (2010).
37. Almeida C. R., Vasconcelos D. P., Goncalves R. M., Barbosa M. A. Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials. J. R. Soc. Interface. 9, 261-271 (2012).
38. Evangelista M. B., et al. Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix. Biomaterials 28, 3644-3655 (2007).
39. Rowley J. A., Madlambayan G., Mooney D. J. Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20, 45-53 (1999).
40. Fonseca K. B., Bidarra S. J., Oliveira M. J., Granja P. L., Barrias C. C. Molecularly designed alginate hydrogels susceptible to local proteolysis as three-dimensional cellular microenvironments. Acta Biomater 7, 1674-1682 (2011).
41. Kuo C. K, Ma P. X. Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties. Biomaterials 22, 511-521 (2001).
42. Antunes J. C., et al. Layer-by-layer self-assembly of chitosan and poly(gamma-glutamic acid) into polyelectrolyte complexes. Biomacromolecules 12, 4183-4195 (2011).
43. Amaral I. F., Sampaio P., Barbosa M. A. Three-dimensional culture of human osteoblastic cells in chitosan sponges: the effect of the degree of acetylation. J. Biomed. Mater. Res. A 76, 335-346 (2006).
44. de Chaumont F., et al. Icy: an open bioimage informatics platform for extended reproducible research. Nat. Methods 9, 690-696 (2012).
45. Otsu N. A Threshold Selection Method from Gray-Level Histograms. Systems, Man and Cybernetics, IEEE Transactions on 9, 62-66 (1979).
46. Olivo-Marin J.-C. Extraction of spots in biological images using multiscale products. Pattern Recognition 35, 1989-1996 (2002).
47. Ollion J., Cochennec J., Loll F., Escude C., Boudier T. TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization. Bioinformatics 29, 1840-1841 (2013).
48. Pinheiro J. C. B. D. M. Mixed-effects models in S and S-PLUS. (Springer, (2000).
49. Richardson A. Multiple Comparisons Using R by Frank Bretz, Torsten Hothorn, Peter Westfall. International Statistical Review 79, 297-297 (2011).
50. Oliveira S. M., Amaral I. F., Barbosa M. A., Teixeira C. C. Engineering endochondral bone: in vitro studies. Tissue Eng. Part A 15, 625-634 (2009).