Long-term detection of fluorescently labeled human mesenchymal stem cell in vitro and in vivo by semi-automated microscopy

Tissue Engineering - Part C: Methods

Volumn 18, Issue 2, 2012, Pages 156-165

  Polzer, Hans ^a   Volkmer, Elias ^a   Saller, Maximilian M ^a,b   Prall, Wolf C ^a   Haasters, Florian ^a   Drosse, Inga ^a   Anz, David ^c   Mutschler, Wolf ^a   Schieker, Matthias ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b MUNICH UNIVERSITY OF APPLIED SCIENCES   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOMATION; BACTERIOPHAGES; CELL CULTURE; FLOW CYTOMETRY; FLUORESCENCE; METABOLISM; STEM CELLS;

CARBOXYFLUORESCEIN; CELL VIABILITY; ENHANCED GREEN FLUORESCENT PROTEIN; FIELD OF VIEWS; FLUORESCENT LABELING; HUMAN MESENCHYMAL STEM CELLS; IN-VITRO; IN-VIVO; LABELED CELLS; LABELING TECHNIQUES; MESENCHYMAL STEM CELL; METABOLIC ACTIVITY; METABOLIC EFFECTS; NUDE MICE; REGENERATIVE MEDICINE; RELATIVE FLUORESCENCE INTENSITY; SEMI-AUTOMATED; SUCCINIMIDYL ESTER; TIME POINTS; TRANSPLANTED CELLS;

SCAFFOLDS (BIOLOGY);

MUS MUSCULUS;

2 (4 IODOPHENYL) 3 (4 NITROPHENYL) 5 (2,4 DISULFOPHENYL) 2H TETRAZOLIUM; 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-(2,4-DISULFOPHENYL)-2H-TETRAZOLIUM; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; TETRAZOLIUM; TISSUE SCAFFOLD;

ALGORITHM; ANIMAL; ARTICLE; AUTOMATION; BONE MATRIX; BONE MINERALIZATION; CELL LINE; CELL SURVIVAL; CELL TRACKING; CHEMISTRY; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; MESENCHYMAL STEM CELL; METABOLISM; METHODOLOGY; MOUSE; NUDE MOUSE; REPRODUCIBILITY; STAINING; TIME;

ALGORITHMS; ANIMALS; AUTOMATION; BONE MATRIX; CALCIFICATION, PHYSIOLOGIC; CELL LINE; CELL SURVIVAL; CELL TRACKING; FLOW CYTOMETRY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; MESENCHYMAL STEM CELLS; MICE; MICE, NUDE; MICROSCOPY, FLUORESCENCE; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING; TETRAZOLIUM SALTS; TIME FACTORS; TISSUE SCAFFOLDS;

EID: 84856153273     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2011.0275     Document Type: Article

References (46)

1. Drosse, I., Volkmer, E., Capanna, R., De Biase, P., Mutschler, W., and Schieker, M. Tissue engineering for bone defect healing: an update on a multi-component approach. Injury 39 Suppl 2, S9, 2008.
2. Schieker, M., Heiss, C., and Mutschler, W. [Bone Substitutes.]. Unfallchirurg 111, 613, 2008.
3. Schieker, M., Seitz, H., Seitz, S., Drosse, I., and Mutschler, W. Biomaterials as scaffold for bone tissue engineering. Eur J Trauma 32, 114, 2010.
4. Kannan, R.Y., Salacinski, H.J., Sales, K., Butler, P., and Seifalian, A.M. The roles of tissue engineering and vascularisation in the development of micro-vascular networks: a review. Biomaterials 26, 1857, 2005.
5. Volkmer, E., Drosse, I., Otto, S., Stangelmayer, A., Stengele, M., Kallukalam, B.C., Mutschler, W., and Schieker, M. Hypoxia in static and dynamic 3D culture systems for tissue engineering of bone. Tissue Eng Part A 14, 1331, 2008.
6. Volkmer, E., Kallukalam, B.C., Maertz, J., Otto, S., Drosse, I., Polzer, H., Bocker, W., Stengele, M., Docheva, D., Mutschler, W., and Schieker, M. Hypoxic preconditioning of human mesenchymal stem cells overcomes hypoxia-induced inhibition of osteogenic differentiation. Tissue Eng Part A 16, 153, 2010.
7. Arkudas, A., Beier, J.P., Heidner, K., Tjiawi, J., Polykandriotis, E., Srour, S., Sturzl, M., Horch, R.E., and Kneser, U. Axial prevascularization of porous matrices using an arteriovenous loop promotes survival and differentiation of transplanted autologous osteoblasts. Tissue Eng 13, 1549, 2007.
8. Kneser, U., Schaefer, D.J., Polykandriotis, E., and Horch, R.E. Tissue engineering of bone: the reconstructive surgeon's point of view. J Cell Mol Med 10, 7, 2006.
9. Himmelreich, U., and Dresselaers, T. Cell labeling and tracking for experimental models using magnetic resonance imaging. Methods 48, 112, 2009.
10. Zhang, Y., Ruel, M., Beanlands, R.S., deKemp, R.A., Suuronen, E.J., and DaSilva, J.N. Tracking stem cell therapy in the myocardium: applications of positron emission tomography. Curr Pharm Des 14, 3835, 2008.
11. Henning, T.D., Boddington, S., and Daldrup-Link, H.E. Labeling HESCs and HMSCs with iron oxide nanoparticles for non-invasive in vivo tracking with MR imaging. J Vis Exp pii: 685. doi: 10.3791/685.2008.
12. Jager, M., Degistirici, O., Knipper, A., Fischer, J., Sager, M., and Krauspe, R. Bone healing and migration of cord bloodderived stem cells into a critical size femoral defect after xenotransplantation. J Bone Miner Res 22, 1224, 2007.
13. Brazelton, T.R., and Blau, H.M. Optimizing techniques for tracking transplanted stem cells in vivo. Stem Cells 23, 1251, 2005.
14. Hemmrich, K., Meersch, M., von Heimburg, D., and Pallua, N. Applicability of the dyes CFSE, CM-DiI and PKH26 for tracking of human preadipocytes to evaluate adipose tissue engineering. Cells Tissues Organs 184, 117, 2006.
15. Rieck, B., Schlaak, S., and Berger, A. [In vivo staining with the fluorescence marker pkh26 in experimental fat cell transplantation. technique and initial results]. Langenbecks Arch Chir Suppl Kongressbd 115, 237, 1998.
16. Rieck, B., and Schlaak, S. In vivo tracking of rat preadipocytes after autologous transplantation. Ann Plast Surg 51, 294, 2003.
17. Kneser, U., Stangenberg, L., Ohnolz, J., Buettner, O., Stern-Straeter, J., Mobest, D., Horch, R.E., Stark, G.B., and Schaefer, D.J. Evaluation of processed bovine cancellous bone matrix seeded with syngenic osteoblasts in a critical size calvarial defect rat model. J Cell Mol Med 10, 695, 2006.
18. Sutton, E.J., Henning, T.D., Pichler, B.J., Bremer, C., and Daldrup-Link, H.E. Cell tracking with optical imaging. Eur Radiol 18, 2021, 2008.
19. Quah, B.J., Warren, H.S., and Parish, C.R. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat Protoc 2, 2049, 2007.
20. Weir, C., Morel-Kopp, M.C., Gill, A., Tinworth, K., Ladd, L., Hunyor, S.N., and Ward, C. Mesenchymal stem cells: isolation, characterisation and in vivo fluorescent dye tracking. Heart Lung Circ 17, 395, 2008.
21. Lyons, A.B. Divided We Stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester. Immunol Cell Biol 77, 509, 1999.
22. Parish, C.R. Fluorescent dyes for lymphocyte migration and proliferation studies. Immunol Cell Biol 77, 499, 1999.
23. Lanzkron, S.M., Collector, M.I., and Sharkis, S.J. Hematopoietic stem cell tracking in vivo: a comparison of short-term and long-term repopulating cells. Blood 93, 1916, 1999.
24. Oostendorp, R.A., Audet, J., and Eaves, C.J. High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro and in vivo. Blood 95, 855, 2000.
25. Dumitriu, I.E., Mohr, W., Kolowos, W., Kern, P., Kalden, J.R., and Herrmann, M. 5,6-Carboxyfluorescein diacetate succinimidyl ester-labeled apoptotic and necrotic as well as detergent-treated cells can be traced in composite cell samples. Anal Biochem 299, 247, 2001.
26. Zimmermann, C.E., Gierloff, M., Hedderich, J., Acil, Y., Wiltfang, J., and Terheyden, H. Survival of transplanted rat bone marrow-derived osteogenic stem cells in vivo. Tissue Eng Part A 17, 1147, 2010.
27. Beavis, A.J., and Pennline, K.J. Tracking of murine spleen cells in vivo: detection of pkh26-labeled cells in the pancreas of non-obese diabetic (NOD) mice. J Immunol Methods 170, 57, 1994.
28. Rieck, B., and Schlaak, S. Measurement in vivo of the survival rate in autologous adipocyte transplantation. Plast Reconstr Surg 111, 2315, 2003.
29. Chen, J., Wang, C., Lu, S., Wu, J., Guo, X., Duan, C., Dong, L., Song, Y., Zhang, J., Jing, D., Wu, L., Ding, J., and Li, D. In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells. Cell Tissue Res 319, 429, 2005.
30. Li, X., Dancausse, H., Grijalva, I., Oliveira, M., and Levi, A.D. Labeling Schwann cells with CFSE-an in vitro and in vivo study. J Neurosci Methods 125, 83, 2003.
31. von Horsten, S., Helfritz, A., Kuhlmann, S., Nave, H., Tschernig, T., Pabst, R., Ben Eliyahu, S., Meyer, D., Schmidt, R.E., and Schmitz, C. Stereological quantification of carboxyfluorescein-labeled rat lung metastasis: a new method for the assessment of natural killer cell activity and tumor adhesion in vivo and in situ. J Immunol Methods 239, 25, 2000.
32. Jomha, N.M., Anoop, P.C., Elliott, J.A., Bagnall, K., and McGann, L.E. Validation and reproducibility of computerised cell-viability analysis of tissue slices. BMC Musculoskelet Disord 4, 5, 2003.
33. Polzer, H., Haasters, F., Prall, W.C., Saller, M.M., Volkmer, E., Drosse, I., Mutschler, W., and Schieker, M. Quantification of fluorescence intensity of labeled human mesenchymal stem cells and cell counting of unlabeled cells in phasecontrast imaging: an open-source-based algorithm. Tissue Eng Part C Methods 16, 1277, 2010.
34. Schieker, M., Pautke, C., Haasters, F., Schieker, J., Docheva, D., Bocker, W., Guelkan, H., Neth, P., Jochum, M., and Mutschler, W. Human mesenchymal stem cells at the singlecell level: simultaneous seven-colour immunofluorescence. J Anat 210, 592, 2007.
35. Bö cker, W., Yin, Z., Drosse, I., Haasters, F., Rossmann, O., Wierer, M., Popov, C., Locher, M., Mutschler, W., Docheva, D., and Schieker, M. Introducing a Single-Cell-Derived Human Mesenchymal Stem Cell Line Expressing HTERT after lentiviral gene transfer. J Cell Mol Med 12, 1347, 2008.
36. Colter, D.C., Class, R., DiGirolamo, C.M., and Prockop, D.J. Rapid expansion of recycling stem cells in cultures of plasticadherent cells from human bone marrow. Proc Natl Acad Sci USA 97, 3213, 2000.
37. Janzen, V., Forkert, R., Fleming, H.E., Saito, Y., Waring, M.T., Dombkowski, D.M., Cheng, T., DePinho, R.A., Sharpless, N.E., and Scadden, D.T. Stem-cell ageing modified by the cyclin-dependent kinase inhibitor P16INK4a. Nature 443, 421, 2006.
38. Seitz, S., Ern, K., Lamper, G., Docheva, D., Drosse, I., Milz, S., Mutschler, W., and Schieker, M. Influence of in vitro cultivation on the integration of cell-matrix constructs after subcutaneous implantation. Tissue Eng 13, 1059, 2007.
39. Abramoff, M.D., Magelhaes, P.J., and Ram, S.J. Image processing with imageJ. Biophotonics Int 11, 36, 2004.
40. Kawarasaki, T., Uchiyama, K., Hirao, A., Azuma, S., Otake, M., Shibata, M., Tsuchiya, S., Enosawa, S., Takeuchi, K., Konno, K., Hakamata, Y., Yoshino, H., Wakai, T., Ookawara, S., Tanaka, H., Kobayashi, E., and Murakami, T. Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source. J Biomed Opt 14, 054017, 2009.
41. Nose, A., and Takeichi, M. A Novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos. J Cell Biol 103, 2649, 1986.
42. Hodgkin, P.D., Lee, J.H., and Lyons, A.B. B Cell differentiation and isotype switching is related to division cycle number. J Exp Med 184, 277, 1996.
43. Hawkins, E.D., Hommel, M., Turner, M.L., Battye, F.L., Markham, J.F., and Hodgkin, P.D. Measuring lymphocyte proliferation, survival and differentiation using CFSE timeseries data. Nat Protoc 2, 2057, 2007.
44. Weston, S.A., and Parish, C.R. New Fluorescent dyes for lymphocyte migration studies. analysis by flow cytometry and fluorescence microscopy. J Immunol Methods 133, 87, 1990.
45. Kneser, U., Polykandriotis, E., Ohnolz, J., Heidner, K., Grabinger, L., Euler, S., Amann, K.U., Hess, A., Brune, K., Greil, P., Sturzl, M., and Horch, R.E. Engineering of vascularized transplantable bone tissues: induction of axial vascularization in an osteoconductive matrix using an arteriovenous loop. Tissue Eng 12, 1721, 2006.
46. Ge, Z., Goh, J.C., and Lee, E.H. The effects of bone marrowderived mesenchymal stem cells and fascia wrap application to anterior cruciate ligament tissue engineering. Cell Transplant 14, 763, 2005.