Comparison between drug screening by immunoassay and ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry in post-mortem urine

Drug Testing and Analysis

Volumn 7, Issue 5, 2015, Pages 420-427

  Sundström, Mira ^a   Pelander, Anna ^a   Ojanperä, Ilkka ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Drug screening; Drugs of abuse; Immunoassay; Time of flight mass spectrometry; Urine

Indexed keywords

DRUG PRODUCTS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOLOGY; INDUCTIVELY COUPLED PLASMA;

DRUG OF ABUSE; DRUG-SCREENING; FALSE POSITIVE; HIGH-RESOLUTION TIME-OF-FLIGHT MASS SPECTROMETRY; IMMUNOASSAY; POST MORTEM; PRESCRIPTION DRUGS; TIME OF FLIGHT MASS SPECTROMETRY; ULTRA-HIGH PERFORMANCE LIQUID CHROMATOGRAPHIES; URINE;

MASS SPECTROMETRY;

AMPHETAMINE DERIVATIVE; BENZODIAZEPINE DERIVATIVE; BUPRENORPHINE; CANNABIS; OPIATE; (22E)-3ALPHA,6ALPHA,7ALPHA,12ALPHA-TETRAHYDROXY-5BETA-CHOL-22-EN-24-OIC ACID; CHOLIC ACID DERIVATIVE; NARCOTIC ANALGESIC AGENT;

ARTICLE; AUTOPSY; CONTROLLED STUDY; DRUG SCREENING; ENZYME IMMUNOASSAY; HUMAN; IMMUNOASSAY; LIMIT OF QUANTITATION; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; SENSITIVITY AND SPECIFICITY; TIME OF FLIGHT MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; URINALYSIS; COMPARATIVE STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROCEDURES; REPRODUCIBILITY; SUBSTANCE ABUSE; URINE;

AMPHETAMINES; ANALGESICS, OPIOID; AUTOPSY; BENZODIAZEPINES; BUPRENORPHINE; CHOLIC ACIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; IMMUNOASSAY; MASS SPECTROMETRY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE DETECTION; URINALYSIS;

EID: 84929133512     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.1683     Document Type: Article

References (27)

1. K.E. Moeller, K.C. Lee, J.C. Kissack . Urine drug screening: Practical guide for clinicians. Mayo Clin. Proc. 2008, 83, 66.
2. P.L. Tenore . Advanced urine toxicology testing. J. Addict. Dis. 2010, 29, 436.
3. A.G. Verstraete, F.V. Heyden . Comparison of the sensitivity and specificity of six immunoassays for the detection of amphetamines in urine. J. Anal. Toxicol. 2005, 29, 359.
4. D.M. Bush . The US mandatory guidelines for federal workplace drug testing programs: Current status and future considerations. Forensic Sci. Int. 2008, 174, 111.
5. M.A. von Mach, C. Weber, M.R. Meyer, L.S. Weilemann, H.H. Maurer, F.T. Peters . Comparison of urinary on-site immunoassay screening and gas chromatography-mass spectrometry results of 111 patients with suspected poisoning presenting at an emergency department. Ther. Drug Monit. 2007, 29, 27.
6. J.C. Eichhorst, M.L. Etter, N. Rousseaux, D.C. Lehotay . Drugs of abuse testing by tandem mass spectrometry: A rapid, simple method to replace immunoassays. Clin. Biochem. 2009, 42, 1531.
7. C. Bell, C. George, A.T. Kicman, A. Traynor . Development of a rapid LC-MS/MS method for direct urinalysis of designer drugs. Drug Test. Anal. 2011, 3, 496.
8. A. Saleh, N.N. Stephanson, I. Granelli, T. Villen, O. Beck . Evaluation of a direct high-capacity target screening approach for urine drug testing using liquid chromatography-time-of-flight mass spectrometry. J. Chromatogr. B 2012, 909, 6.
9. A. de Castro, M. Gergov, P. Östman, I. Ojanperä, A. Pelander . Combined drug screening and confirmation by liquid chromatography time-of-flight mass spectrometry with reverse database search. Anal. Bioanal. Chem. 2012, 403, 1265.
10. S. Broecker, S. Herre, B. Wüst, J. Zweigenbaum, F. Pragst . Development and practical application of a library of CID accurate mass spectra of more than 2500 toxic compounds for systematic toxicological analysis by LC-QTOF-MS with data-dependent acquisition. Anal. Bioanal. Chem. 2011, 400, 101.
11. R.I.D. Birkler, R. Telving, O. Ingemann-Hansen, A.V. Charles, M. Johannsen, M.F. Andreasen . Screening analysis of drugs and drugs of abuse in whole blood using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) - Toxicological findings in cases of alleged sexual assault. Forensic Sci. Int. 2012, 222, 154.
12. H.K. Lee, C.S. Ho, Y.P.H. Iu, P.S.J. Lai, C.C. Shek, Y.-C. Lo, H.B. Klinke, M. Wood . Development of a broad toxicological screening technique for urine using ultra-performance liquid chromatography and time-of-flight mass spectrometry. Anal. Chim. Acta 2009, 649, 80.
13. F. Guale, S. Shahreza, J.P. Walterscheid, H.-H. Chen, C. Arndt, A.T. Kelly, A. Mozayani . Validation of LC-TOF-MS screening for drugs, metabolites, and collateral compounds in forensic toxicology specimens. J. Anal. Toxicol. 2013, 37, 17.
14. M. Roman, L. Ström, H. Tell, M. Josefsson . Liquid chromatography/time-of-flight mass spectrometry analysis of post-mortem blood samples for targeted toxicological screening. Anal. Bioanal. Chem. 2013, 405, 4107.
15. I. Ojanperä, M. Kolmonen, A. Pelander . Current use of high-resolution mass spectrometry in drug screening relevant to clinical and forensic toxicology and doping control. Anal. Bioanal. Chem. 2012, 403, 1203.
16. M. Sundström, A. Pelander, V. Angerer, M. Hutter, S. Kneisel, I. Ojanperä . A high-sensitivity ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry (UHPLC-HR-TOFMS) method for screening synthetic cannabinoids and other drugs of abuse in urine. Anal. Bioanal. Chem. 2013, 405, 8463.
17. E. Vuori, M. Happonen, M. Gergov, T. Nenonen, A. Järvinen, R.A. Ketola, R. Vahala . Wastewater analysis reveals regional variability in exposure to abused drugs and opioids in Finland. Sci. Total Environ. 2014, 487, 688.
18. A.H. Wu, T.A. Onigbinde, S.S. Wong, K.G. Johnson . Identification of methamphetamines and over-the-counter sympathometic amines by full-scan GC-ion trap MS with electron impact and chemical ionization. J. Anal. Toxicol. 1992, 16, 137.
19. S. Mykkänen, J. Seppälä, K. Ariniemi, P. Lillsunde . GCD quantitation of opiates as propionyl derivatives in blood. J. Anal. Toxicol. 2000, 24, 122.
20. IST. Application Note IST 1001: SPE Application Note for Extraction of THC-COOH from Urine (THC Column). International Sorbent Technology Ltd, Hengoed, Caerffili, United Kingdom, 1997.
21. J.H. Lewis, J. Bini, P. Bowron, J.C. Wadsworth . Profiling benzodiazepine abuse by GC/MS. The International Association of Forensic Toxicologists, Proceedings of the 34th Triennial Meeting. Interlaken 1996, MOLINApress, Leipzig, 1997, pp. 259-261.
22. R. Meatherall . Optimal Enzymatic hydrolysis of urinary benzodiazepine conjugates. J. Anal. Toxicol. 1994, 18, 382.
23. M. Gergov, P. Nokua, E. Vuori, I. Ojanperä . Simultaneous screening and quantification of 25 opioid drugs in post-mortem blood and urine by liquid chromatography-tandem mass spectrometry. Forensic Sci. Int. 2009, 186, 36.
24. A. Arntson, B. Ofsa, D. Lancaster, J.R. Simon, M. McMullin, B. Logan . Validation of a novel immunoassay for the detection of synthetic cannabinoids and metabolites in urine specimens. J. Anal. Toxicol. 2013, 37, 284.
25. I.A. Lua, S.-L. Lin, H.R. Lin, A.C. Lua . Replacing immunoassays for mephedrone, ketamines and six amphetamine-type stimulants with flow injection analysis tandem mass spectrometry. J. Anal. Toxicol. 2012, 36, 575.
26. I.-L. Tsai, T.-I. Weng, Y.J. Tseng, H.K.-L. Tan, H.-J. Sun, C.-H. Kuo . Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography - quadrupole time-of-flight mass spectrometry. J. Anal. Toxicol. 2013, 37, 642.
27. R. Kronstrand, L. Brinkhagen, C. Birath-Karlsson, M. Roman, M. Josefsson . LC-QTOF-MS as a superior strategy to immunoassay for the comprehensive analysis of synthetic cannabinoids in urine. Anal. Bioanal. Chem. 2014, 406, 3599.