High-throughput, quantitative enzyme kinetic analysis in microdroplets using stroboscopic epifluorescence imaging

Analytical Chemistry

Volumn 87, Issue 9, 2015, Pages 4965-4972

  Hess, David ^a   Rane, Anandkumar ^a   Demello, Andrew J ^a   Stavrakis, Stavros ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DISSOCIATION; ENZYME KINETICS; ENZYMES; FLUIDIC DEVICES; KINETICS; LASER EXCITATION; MICROFLUIDICS; REACTION KINETICS;

DISSOCIATION CONSTANT; DROPLET-BASED MICROFLUIDICS; EPI-FLUORESCENCE IMAGING; FLUOROGENIC SUBSTRATE; HIGH-TIME RESOLUTION; INDIVIDUAL DROPLETS; KINETIC MEASUREMENT; MICROFLUIDIC CHANNEL;

DROPS;

BETA GALACTOSIDASE;

ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; KINETICS; LASER; METABOLISM; MICROFLUIDIC ANALYSIS; PARTICLE SIZE; PROCEDURES; SURFACE PROPERTY;

BETA-GALACTOSIDASE; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; HIGH-THROUGHPUT SCREENING ASSAYS; KINETICS; LASERS; MICROFLUIDIC ANALYTICAL TECHNIQUES; PARTICLE SIZE; SURFACE PROPERTIES;

EID: 84928920298     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/acs.analchem.5b00766     Document Type: Article

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