Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Journal of the American Chemical Society

Volumn 133, Issue 27, 2011, Pages 10368-10371

  Chen, Chia Hung ^a   Sarkar, Aniruddh ^a   Song, Yong Ak ^a,b   Miller, Miles A ^b   Kim, Sung Jae ^a   Griffith, Linda G ^b   Lauffenburger, Douglas A ^b   Han, Jongyoon ^a,b  

^a USA   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DROPLET-BASED MICROFLUIDICS; ENZYME ASSAYS; INTEGRATED MICROFLUIDIC DEVICES; LIMITED SENSITIVITY; MATRIX METALLOPROTEINASES; MICRO DROPLETS; MICRO REACTOR; PROTEASE ACTIVITIES; SAMPLE VOLUME;

ASSAYS; BIOMOLECULES; CHEMICAL REACTORS; CONCENTRATION (PROCESS); DROPS;

MICROFLUIDICS;

BETA GALACTOSIDASE; GELATINASE B; MATRIX METALLOPROTEINASE; PROTEINASE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DEVICE; EMBRYO; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; FIBROBLAST CULTURE; FLUORESCENCE; MICROFLUIDICS; MOLECULE; MOUSE; NANOFLUIDICS; NONHUMAN; PROTEIN DEGRADATION; REACTION TIME; TECHNOLOGY;

ANIMALS; CELLS, CULTURED; FIBROBLASTS; MATRIX METALLOPROTEINASES; MICE; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROFLUIDICS; SENSITIVITY AND SPECIFICITY;

EID: 79960068110     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja2036628     Document Type: Article

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