Mutagenesis of Trichoderma reesei endoglucanase I: Impact of expression host on activity and stability at elevated temperatures

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Chokhawala, Harshal A ^a   Roche, Christine M ^a   Kim, Tae Wan ^a   Atreya, Meera E ^a   Vegesna, Neeraja ^b   Dana, Craig M ^a   Blanch, Harvey W ^a   Clark, Douglas S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b INDIAN INSTITUTE OF TECHNOLOGY   (India)

Author keywords

B factor; Cell free synthesis; Cellulase; Endoglucanase I; Glutaminyl cylcase; Neurospora crassa; Pyroglutamate; Saccharomyces cerevisiae; Thermostability; Trichoderma reesei

Indexed keywords

AMINO ACIDS; CELLULOSE; ENGINEERS; ENZYMES; ESCHERICHIA COLI; LIGNIN; SACCHARIFICATION; STABILITY; YEAST;

B FACTORS; CELL FREE SYNTHESIS; CELLULASE; ENDOGLUCANASE I; GLUTAMINYL; NEUROSPORA CRASSA; PYROGLUTAMATE; THERMOSTABILITY; TRICHODERMA REESEI;

C (PROGRAMMING LANGUAGE);

CELLULOSE; ENDOGLUCANASE 1; GLUCAN SYNTHASE; GLUTAMINE; PYROGLUTAMIC ACID; UNCLASSIFIED DRUG; CELLULASE; FUNGAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME MODIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SYNTHESIS; ESCHERICHIA COLI; HIGH TEMPERATURE; HYPOCREA JECORINA; MUTAGENESIS; NEUROSPORA CRASSA; NONHUMAN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY; CHEMISTRY; ENZYMOLOGY; GENETICS; HALF LIFE TIME; HEAT; METABOLISM; MOLECULAR MODEL; PROTEIN CONFORMATION; SPECIES DIFFERENCE; TRICHODERMA;

CELLULASE; CELLULOSE; ENZYME STABILITY; ESCHERICHIA COLI; FUNGAL PROTEINS; HALF-LIFE; HOT TEMPERATURE; MODELS, MOLECULAR; MUTAGENESIS; NEUROSPORA CRASSA; PROTEIN CONFORMATION; PYRROLIDONECARBOXYLIC ACID; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SPECIES SPECIFICITY; TRICHODERMA;

EID: 84928728061     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0118-z     Document Type: Article

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