Biased clique shuffling reveals stabilizing mutations in cellulase Cel7A

Biotechnology and Bioengineering

Volumn 109, Issue 11, 2012, Pages 2710-2719

  Dana, Craig M ^a   Saija, Poonam ^a   Kal, Sarala M ^a   Bryan, Mara B ^a   Blanch, Harvey W ^a   Clark, Douglas S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cel7A CBHI; Cellulase; Directed evolution; DNA shuffling; Enzyme thermostability; Yeast expression

Indexed keywords

CEL7A CBHI; CELLULASE; DIRECTED EVOLUTION; DNA SHUFFLING; YEAST EXPRESSION;

CELL MEMBRANES; GENES; SACCHARIFICATION;

YEAST;

ADENOSINE TRIPHOSPHATASE; CELLULASE; GLYCOSIDASE; PROTEIN DISULFIDE ISOMERASE;

ARTICLE; BIASED CLIQUE SHUFFLING; CELL MEMBRANE; CHIMERA; DNA SHUFFLING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME STABILITY; FUNGAL STRAIN; GENE DELETION; GENE MUTATION; GLYCOSYLATION; HIGH THROUGHPUT SCREENING; HYDROPHILICITY; MOLECULAR LIBRARY; MUTAGENESIS; NONHUMAN; SACCHAROMYCES CEREVISIAE; TEMPERATURE; THERMOSTABILITY; UPREGULATION;

CELLULASE; DNA SHUFFLING; ENZYME STABILITY; METABOLIC ENGINEERING; MUTAGENESIS; MUTATION, MISSENSE; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 84866759500     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24708     Document Type: Article

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