High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  He, Yong Zhi ^a,b   Gong, Jiao ^a   Yu, Hai Ying ^a   Tao, Yong ^a   Zhang, Shan ^a   Dong, Zhi Yang ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Aspartate; Ectoine; Escherichia coli; Glycerol; Halomonas elongata; Whole cell biocatalysis

Indexed keywords

ASPARTATE SODIUM; ASPARTIC ACID; ECTOINE; GLYCEROL; SODIUM DIHYDROGEN PHOSPHATE; 2,4-DIAMINOBUTYRIC ACID; AMINOBUTYRIC ACID DERIVATIVE; AMINOTRANSFERASE; BACTERIAL PROTEIN; DIAMINO ACID; ECTOINE SYNTHASE; HYDROLYASE;

ALKALINITY; ARTICLE; BIOCATALYSIS; BIOCATALYST; BIOMASS; BIOMASS PRODUCTION; BIOSYNTHESIS; BIOTRANSFORMATION; CELL DENSITY; CELL SUSPENSION; CLONING; CONCENTRATION (PARAMETERS); DRY WEIGHT; ESCHERICHIA COLI K 12; FLOW RATE; GENE ACTIVITY; GENE CASSETTE; GENE EXPRESSION; GENE SEQUENCE; GENETIC STABILITY; HALOMONAS; HALOMONAS ELONGATA; MOLECULAR WEIGHT; NONHUMAN; OPERON; PROCESS OPTIMIZATION; REACTION TIME; WHOLE CELL; BIOREACTOR; CYTOLOGY; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGY; PROCEDURES; REPRODUCIBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ESCHERICHIA COLI K12; HALOMONAS ELONGATA;

AMINO ACIDS, DIAMINO; AMINOBUTYRATES; ASPARTIC ACID; BACTERIAL PROTEINS; BIOCATALYSIS; BIOMASS; BIOREACTORS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI K12; GENETIC ENGINEERING; GLYCEROL; HALOMONAS; HYDRO-LYASES; INDUSTRIAL MICROBIOLOGY; MASS SPECTROMETRY; OPERON; REPRODUCIBILITY OF RESULTS; TRANSAMINASES;

EID: 84928266661     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0238-0     Document Type: Article

References (33)

1. Oren A, Heldal M, Norland S, Galinski EA. Intracellular ion and organic solute concentrations of the extremely halophilic bacterium Salinibacter ruber. Extremophiles. 2002;6:491-8.
2. Roberts MF. Osmoadaptation and osmoregulation in archaea: update 2004. Front Biosci. 2004;9:1999-2019.
3. Kempf B, Bremer E. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol. 1998;170:319-30.
4. Galinski EA, PFEIFFER HP, TRÜPER HG. 1, 4, 5, 6-Tetrahydro-2-methyl-4-pyrimidinecarboxylic acid. Eur J Biochem. 1985;149:135-9.
5. Lentzen G, Schwarz T. Extremolytes: Natural compounds from extremophiles for versatile applications. Appl Microbiol Biot. 2006;72:623-34.
6. Da Costa MS, Santos H, Galinski EA. An overview of the role and diversity of compatible solutes in Bacteria and Archaea. Adv Biochem Eng Biotechnol. 1998;61:117-53.
7. Graf R, Anzali S, Buenger J, Pfluecker F, Driller H. The multifunctional role of ectoine as a natural cell protectant. Clin Dermatol. 2008;26:326-33.
8. Jorg Kunte H, Lentzen G,A, Galinski E. Industrial production of the cell protectant ectoine: protection mechanisms, processes, and products. Curr Biotechnol. 2014;3:10-25.
9. Abdel-Aziz H, Wadie W, Abdallah DM, Lentzen G, Khayyal MT. Novel effects of ectoine, a bacteria-derived natural tetrahydropyrimidine, in experimental colitis. Phytomedicine. 2013;20:585-91.
10. Pastor JM, Salvador M, Argandona M, Bernal V, Reina-Bueno M, Csonka LN, et al. Ectoines in cell stress protection: uses and biotechnological production. Biotechnol Adv. 2010;28:782-801.
11. Cánovas D, Vargas C, Iglesias-Guerra F, Csonka LN, Rhodes D, Ventosa A, et al. Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes. J Biol Chem. 1997;272:25794-801.
12. Peters P, Galinski EA, Truper HG. The Biosynthesis of Ectoine. FEMS Microbiol Lett. 1990;71:157-62.
13. Schwibbert K, MarinSanguino A, Bagyan I, Heidrich G, Lentzen G, Seitz H, et al. A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T. Environ Microbiol. 2011;13:1973-94.
14. Sauer T, Galinski EA. Bacterial milking: a novel bioprocess for production of compatible solutes. Biotechnol Bioeng. 1998;57:306-13.
15. Fallet C, Rohe P, Franco-Lara E. Process optimization of the integrated synthesis and secretion of ectoine and hydroxyectoine under hyper/hypo-osmotic stress. Biotechnol Bioeng. 2010;107:124-33.
16. Schubert T, Maskow T, Benndorf D, Harms H, Breuer U. Continuous synthesis and excretion of the compatible solute ectoine by a transgenic, nonhalophilic bacterium. Appl Environ Microbiol. 2007;73:3343-7.
17. Zhang LH, Lang YJ, Nagata S. Efficient production of ectoine using ectoine-excreting strain. Extremophiles. 2009;13:717-24.
18. Bestvater T, Louis P, Galinski EA. Heterologous ectoine production in Escherichia coli: by-passing the metabolic bottle-neck. Saline Systems. 2008;4:12.
19. Stöveken N, Pittelkow M, Sinner T, Jensen RA, Heider J, Bremer E. A specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in Pseudomonas stutzeri A1501. J Bacteriol. 2011;193:4456-68.
20. Anbu Rajan L, Joseph TC, Thampuran N, James R, Ashok Kumar K, Viswanathan C, et al. Cloning and heterologous expression of ectoine biosynthesis genes from Bacillus halodurans in Escherichia coli. Biotechnol Lett. 2008;30:1403-7.
21. Zhu D, Liu J, Han R, Shen G, Long Q, Wei X, et al. Identification and characterization of ectoine biosynthesis genes and heterologous expression of the ectABC gene cluster from Halomonas sp. QHL1, a moderately halophilic bacterium isolated from Qinghai Lake. J Microbiol. 2014;52:139-47.
22. Becker J, Schafer R, Kohlstedt M, Harder BJ, Borchert NS, Stoveken N, et al. Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microb Cell Fact. 2013;12:110.
23. Eilert E, Kranz A, Hollenberg CP, Piontek M, Suckow M. Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha. J Biotechnol. 2013;167:85-93.
24. Duetz WA, van Beilen JB, Witholt B. Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis. Curr Opin Biotechnol. 2001;12:419-25.
25. Blattner FR, Plunkett 3rd G, Bloch CA, Perna NT, Burland V, Riley M, et al. The complete genome sequence of Escherichia coli K-12. Science. 1997;277:1453-62.
26. Jebbar M, Talibart R, Gloux K, Bernard T, Blanco C. Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics. J Bacteriol. 1992;174:5027-35.
27. Göller K, Ofer A, Galinski EA. Construction and characterization of an NaCl-sensitive mutant of Halomonas elongata impaired in ectoine biosynthesis. FEMS Microbiol Lett. 1998;161:293-300.
28. Ono H, Sawada K, Khunajakr N, Tao T, Yamamoto M, Hiramoto M, et al. Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata. J Bacteriol. 1999;181:91-9.
29. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor; 2001.
30. Sechi S, Chait BT. Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification. Anal Chem. 1998;70:5150-8.
31. Liu H, Luo Y, Han J, Wu J, Wu Z, Feng D, et al. Proteome reference map of Haloarcula hispanica and comparative proteomic and transcriptomic analysis of polyhydroxyalkanoate biosynthesis under genetic and environmental perturbations. J Proteome Res. 2013;12:1300-15.
32. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959;37:911-7.
33. Mengerink Y, Kutlán D, Tóth F, Csámpai A, Molnár-Perl I. Advances in the evaluation of the stability and characteristics of the amino acid and amine derivatives obtained with the o-phthaldialdehyde/3-mercaptopropionic acid and o-phthaldialdehyde/N-acetyl-L-cysteine reagents: High-performance liquid chromatography-mass spectrometry study. J Chromatogr A. 2002;949:99-124.