2000-fold parallelized dual-color STED fluorescence nanoscopy

Optics Express

Volumn 23, Issue 1, 2015, Pages 211-223

  Bergermann, Fabian ^a   Alber, Lucas ^a   Sahl, Steffen J ^b   Engelhardt, Johann ^a   Hell, Stefan W ^a,b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; STIMULATED EMISSION;

CIRCULAR FIELD OF VIEWS; FLUORESCENCE SWITCH; HIGH SPEED DETECTORS; IMAGING CAPABILITIES; MULTICOLOR FLUORESCENCE; PARALLELIZATIONS; SPATIO-TEMPORAL RESOLUTION; STIMULATED EMISSION DEPLETION;

COLOR;

EID: 84928203283     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.23.000211     Document Type: Article

References (33)

1. S. W. Hell and J. Wichmann, "Breaking the diffraction resolution limit by stimulated emission: stimulatedemission-depletion fluorescence microscopy," Opt. Lett. 19(11), 780-782 (1994).
2. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, "Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission," Proc. Natl. Acad. Sci. U.S.A. 97(15), 8206-8210 (2000).
3. S. W. Hell, "Toward fluorescence nanoscopy," Nat. Biotechnol. 21(11), 1347-1355 (2003).
4. M. J. Rust, M. Bates, and X. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Methods 3(10), 793-796 (2006).
5. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, "Imaging Intracellular Fluorescent Proteins at Nanometer Resolution," Science 313(5793), 1642-1645 (2006).
6. S. T. Hess, T. P. K. Girirajan, and M. D. Mason, "Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy," Biophys. J. 91(11), 4258-4272 (2006).
7. J. Fölling, M. Bossi, H. Bock, R. Medda, C. A. Wurm, B. Hein, S. Jakobs, C. Eggeling, and S. W. Hell, "Fluorescence nanoscopy by ground-state depletion and single-molecule return," Nat. Methods 5(11), 943-945 (2008).
8. M. Hofmann, C. Eggeling, S. Jakobs, and S. W. Hell, "Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins," Proc. Natl. Acad. Sci. U.S.A. 102(49), 17565-17569 (2005).
9. E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, "STED microscopy reveals crystal colour centres with nanometric resolution," Nat. Photonics 3(3), 144-147 (2009).
10. S. Berning, K. I. Willig, H. Steffens, P. Dibaj, and S. W. Hell, "Nanoscopy in a Living Mouse Brain," Science 335(6068), 551 (2012).
11. F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, and S. W. Hell, "Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution," Biophys. J. 105(1), L01-L03 (2013).
12. G. Lukinavičius, L. Reymond, E. D'Este, A. Masharina, F. Göttfert, H. Ta, A. Güther, M. Fournier, S. Rizzo, H. Waldmann, C. Blaukopf, C. Sommer, D. W. Gerlich, H.-D. Arndt, S. W. Hell, and K. Johnsson, "Fluorogenic probes for live-cell imaging of the cytoskeleton," Nat. Methods 11(7), 731-733 (2014).
13. V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, and S. W. Hell, "Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement," Science 320(5873), 246-249 (2008).
14. S. W. Hell, "Microscopy and its focal switch," Nat. Methods 6(1), 24-32 (2009).
15. RESOLFT: Reversible Saturable/Switchable Optically Linear (Fluorescence) Transitions.
16. T. Grotjohann, I. Testa, M. Leutenegger, H. Bock, N. T. Urban, F. Lavoie-Cardinal, K. I. Willig, C. Eggeling, S. Jakobs, and S. W. Hell, "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP," Nature 478(7368), 204-208 (2011).
17. A. Chmyrov, J. Keller, T. Grotjohann, M. Ratz, E. d'Este, S. Jakobs, C. Eggeling, and S. W. Hell, "Nanoscopy with more than 100,000 'doughnuts'," Nat. Methods 10(8), 737-740 (2013).
18. C. A. Wurm, K. Kolmakov, F. Göttfert, H. Ta, M. Bossi, H. Schill, S. Berning, S. Jakobs, G. Donnert, V. N. Belov, and S. W. Hell, "Novel red fluorophores with superior performance in STED microscopy," Opt. Nanoscopy 1(1), 1-7 (2012).
19. P. Bingen, M. Reuss, J. Engelhardt, and S. W. Hell, "Parallelized STED fluorescence nanoscopy," Opt. Express 19(24), 23716-23726 (2011).
20. B. Yang, F. Przybilla, M. Mestre, J.-B. Trebbia, and B. Lounis, "Large parallelization of STED nanoscopy using optical lattices," Opt. Express 22(5), 5581-5589 (2014).
21. M. G. Gustafsson, "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy," J. Microsc. 198(2), 82-87 (2000).
22. M. G. Gustafsson, "Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution," Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081-13086 (2005).
23. E. H. Rego, L. Shao, J. J. Macklin, L. Winoto, G. A. Johansson, N. Kamps-Hughes, M. W. Davidson, and M. G. L. Gustafsson, "Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution," Proc. Natl. Acad. Sci. U.S.A. 109(3), E135-E143 (2012).
24. F. Görlitz, P. Hoyer, H. Falk, L. Kastrup, J. Engelhardt, and S. W. Hell, "A STED Microscope Designed for Routine Biomedical Applications," Prog. Electromagnetics Res. 147, 57-68 (2014).
25. G. Vicidomini, A. Schönle, H. Ta, K. Y. Han, G. Moneron, C. Eggeling, and S. W. Hell, "STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects," PLoS ONE 8(1), e54421 (2013).
26. B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, and S. W. Hell, "Resolution scaling in STED microscopy," Opt. Express 16(6), 4154-4162 (2008).
27. J. Bückers, D. Wildanger, G. Vicidomini, L. Kastrup, and S. W. Hell, "Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses," Opt. Express 19(4), 3130-3143 (2011).
28. G. Donnert, C. Eggeling, and S. W. Hell, "Major signal increase in fluorescence microscopy through dark-state relaxation," Nat. Methods 4(1), 81-86 (2007).
29. G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, and S. W. Hell, "Macromolecular-scale resolution in biological fluorescence microscopy," Proc. Natl. Acad. Sci. U.S.A. 103(31), 11440-11445 (2006).
30. G. Porter, P. J. Sadkowski, and C. J. Tredwell, "Picosecond rotational diffusion in kinetic and steady state fluorescence spectroscopy," Chem. Phys. Lett. 49(3), 416-420 (1977).
31. M. Dyba and S. W. Hell, "Focal spots of size λ/23 open up far-field fluorescence microscopy at 33 nm axial resolution," Phys. Rev. Lett. 88(16), 163901 (2002).
32. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system," Proc. R. Soc. Lond. Ser. Math. Phys. Sci. 253, 358-379 (1959).
33. Y. Maruyama and E. Charbon, "A time-gated 128×128 CMOS SPAD array for on-chip fluorescence detection," in Proc. Intl. Image Sensor Workshop (IISW) (2011), pp. 270-273.