Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells

International Journal of Nanomedicine

Volumn 10, Issue , 2015, Pages 1841-1854

  Lee, Kunwoo ^a,b   Yu, Pengzhi ^c   Lingampalli, Nithya ^a   Kim, Hyun Jin ^a   Tang, Richard ^a   Murthy, Niren ^a,b  

^a University of California at Berkeley   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

Author keywords

Cardiac fibroblast transfection; Cardiac regeneration; Direct cardiac reprogramming; MRNA transfection

Indexed keywords

ALPHA ACTININ; GREEN FLUORESCENT PROTEIN; LIPOFECTAMINE; MESSENGER RNA; POLYARGININE; POLYPEPTIDE; CYSTEINYL-ARGINYL-PROLYL-PROLYL-ARGININE; GENETIC MARKER; LIPID; OLIGOPEPTIDE; RECOMBINANT PROTEIN; T BOX TRANSCRIPTION FACTOR; T-BOX TRANSCRIPTION FACTOR 5;

ACTC2 GENE; ACTN2 GENE; ALPHA MHC GENE; ANIMAL CELL; ARTICLE; CELL STRUCTURE; COMPLEX FORMATION; CONTROLLED STUDY; FIBROBLAST; GENE; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GJA1 GENE; HAND2 GENE; HEART MUSCLE CELL; IMMUNOHISTOCHEMISTRY; MOLECULAR DYNAMICS; MOUSE; NEWBORN; NONHUMAN; NUCLEAR REPROGRAMMING; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; RNA TRANSPORT; SIGNAL TRANSDUCTION; TNNT2 GENE; UPREGULATION; ANIMAL; CARDIAC MUSCLE; CARDIAC MUSCLE CELL; CELL CULTURE; CHEMISTRY; CYTOLOGY; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETIC TRANSFECTION; GENETICS; METABOLISM; PHYSIOLOGY; PROCEDURES; TRANSGENIC MOUSE; ULTRASTRUCTURE;

ANIMALS; CELLS, CULTURED; CELLULAR REPROGRAMMING; FIBROBLASTS; GENE EXPRESSION REGULATION; GENETIC MARKERS; GREEN FLUORESCENT PROTEINS; LIPIDS; MICE, TRANSGENIC; MYOCARDIUM; MYOCYTES, CARDIAC; OLIGOPEPTIDES; RECOMBINANT PROTEINS; RNA, MESSENGER; T-BOX DOMAIN PROTEINS; TRANSFECTION;

EID: 84928137597     PISSN: 11769114     EISSN: 11782013     Source Type: Journal    
DOI: 10.2147/IJN.S75124     Document Type: Article

References (24)

1. Qian L, Huang Y, Spencer C, et al. In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature. 2012;485(7400):593–598.
2. Ieda M, Fu JD, Delgado-Olguin P, et al. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell. 2010;142(3):375–386.
3. Song K, Nam YJ, Luo X, et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 2012;485(7400):599–604.
4. Warren L, Manos PD, Ahfeldt T, et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 2010;7(5):618–630.
5. Zhou H, Wu S, Joo J, et al. Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell. 2009;4(5):381–384.
6. Adler AF, Grigsby CL, Kulangara K, Wang H, Yasuda R, Leong KW. Nonviral direct conversion of primary mouse embryonic fibroblasts to neuronal cells. Mol Ther Nucleic Acids. 2012;1:e32.
7. Warren L, Ni Y, Wang J, Guo X. Feeder-free derivation of human induced pluripotent stem cells with messenger RNA. Sci Rep. 2012;2:657.
8. Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S. Generation of mouse induced pluripotent stem cells without viral vectors. Science. 2008;322(5903):949–953.
9. Mandal PK, Rossi DJ. Reprogramming human fibroblasts to pluripotency using modified mRNA. Nat Protoc. 2013;8(3):568–582.
10. Yakubov E, Rechavi G, Rozenblatt S, Givol D. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors. Biochem Biophys Res Commun. 2010;394(1):189–193.
11. Kim D, Kim CH, Moon JI, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. 2009;4(6):472–476.
12. Kormann MS, Hasenpusch G, Aneja MK, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nat Biotechnol. 2011;29(2):154–157.
13. Bettinger T, Carlisle RC, Read ML, Ogris M, Seymour LW. Peptide-mediated RNA delivery: a novel approach for enhanced transfection of primary and post-mitotic cells. Nucleic Acids Res. 2001;29(18):3882–3891.
14. Wada R, Muraoka N, Inagawa K, et al. Induction of human cardiomyocyte-like cells from fibroblasts by defined factors. Proc Natl Acad Sci U S A. 2013;110(31):12667–12672.
15. Laeremans H, Rensen SS, Ottenheijm HC, Smits JF, Blankesteijn WM. Wnt/frizzled signalling modulates the migration and differentiation of immortalized cardiac fibroblasts. Cardiovasc Res. 2010;87(3):514–523.
16. Subramanian M, Lim J, Dobson J. Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes. PLoS One. 2013;8(7):e69812.
17. Drews K, Tavernier G, Demeester J, et al. The cytotoxic and immunogenic hurdles associated with non-viral mRNA-mediated reprogramming of human fibroblasts. Biomaterials. 2012;33(16):4059–4068.
18. Zhang L, Hoffman JA, Ruoslahti E. Molecular profiling of heart endothelial cells. Circulation. 2005;112(11):1601–1611.
19. Zhang H, Li N, Sirish P, et al. The cargo of CRPPR-conjugated liposomes crosses the intact murine cardiac endothelium. J Control Release. 2012;163(1):10–17.
20. Qian L, Berry EC, Fu JD, Ieda M, Srivastava D. Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro. Nat Protoc. 2013;8(6):1204–1215.
21. Carey BW, Markoulaki S, Hanna JH, et al. Reprogramming factor stoichiometry influences the epigenetic state and biological properties of induced pluripotent stem cells. Cell Stem Cell. 2011;9(6):588–598.
22. Papapetrou EP, Tomishima MJ, Chambers SM, et al. Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. 2009;106(31):12759–12764.
23. Bisping E, Ikeda S, Sedej M, et al. Transcription factor GATA4 is activated but not required for insulin-like growth factor 1 (IGF1)-induced cardiac hypertrophy. J Biol Chem. 2012;287(13):9827–9834.
24. Bruneau BG, Nemer G, Schmitt JP, et al. A murine model of Holt-Oram syndrome defines roles of the T-box transcription factor Tbx5 in cardiogenesis and disease. Cell. 2001;106(6):709–721.