New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli

Applied and Environmental Microbiology

Volumn 81, Issue 9, 2015, Pages 3243-3254

  Schwendener, Sybille ^a   Perreten, Vincent ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CLONING; ESCHERICHIA COLI; GENES; PROTEINS;

CONSTITUTIVE PROMOTERS; EXPRESSION SYSTEM; MOLECULAR MANIPULATION; POLYHISTIDINE-TAGGED FUSION PROTEIN; PROMOTER UPSTREAM; PROTEIN PURIFICATION; STAPHYLOCOCCUS AUREUS; TRANSLATIONAL FUSION;

GENE EXPRESSION;

CLONE; COLIFORM BACTERIUM; CYTOLOGY; GENE EXPRESSION; MOLECULAR ANALYSIS; PROTEIN;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

BACTERIAL DNA; HYBRID PROTEIN; METHYLTRANSFERASE; RRNA (ADENOSINE-O-2'-)METHYLTRANSFERASE;

AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CHEMISTRY; DNA REPLICATION ORIGIN; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR BIOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PLASMID; PROCEDURES; PROMOTER REGION; REPLICON; STAPHYLOCOCCUS AUREUS;

CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; METHYLTRANSFERASES; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; REPLICATION ORIGIN; REPLICON; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCUS AUREUS;

EID: 84927795067     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03803-14     Document Type: Article

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