Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species

Applied and Environmental Microbiology

Volumn 69, Issue 6, 2003, Pages 3385-3392

  Schofield, David A ^a   Westwater, Caroline ^a   Hoel, Brian D ^a   Werner, Phillip A ^a   Norris, James S ^a   Schmidt, Michael G ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGES; GENES; GENETIC ENGINEERING;

GENE EXPRESSION;

ESCHERICHIA COLI;

BACTERIUM; GENE EXPRESSION; MICROBIOLOGY;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; GENE EXPRESSION REGULATION; GENE INDUCTION; GENETIC CONSERVATION; GRAM POSITIVE BACTERIUM; HOSPITAL INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PROMOTER REGION; REPORTER GENE; REPRESSOR GENE; SPECIES DIFFERENTIATION; TEMPERATURE SENSITIVE MUTANT; THERMOREGULATION;

ANIMALIA; BACTERIA (MICROORGANISMS); BROAD HOST RANGE PLASMIDS; ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; UNIDENTIFIED BACTERIOPHAGE;

EID: 0037979019     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.6.3385-3392.2003     Document Type: Article

References (56)

1. Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857.
2. Behnke, D., M. S. Gilmore, and J. Ferretti. 1982. pGB301 vector plasmid family and its use for molecular cloning in streptococci, p. 239-242. In D. Schlessinger (ed.), Microbiology. American Society for Microbiology, Washington, D.C.
3. Citron, M., M. Velleman, and H. Schuster. 1989. Three additional operators, Op21, Op68, and Op88, of bacteriophage P1. Evidence for control of the P1 dam methylase by Op68. J. Biol. Chem. 264:3611-3617.
4. de Ruyter, P. G., O. P. Kuipers, and W. M. de Vos. 1996. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl. Environ. Microbiol. 62:3662-3667.
5. Deuschle, U., R. Gentz, and H. Bujard. 1986. lac repressor blocks transcribing RNA polymerase and terminates transcription. Proc. Natl. Acad. Sci. USA 83:4134-4137.
6. Eichenbaum, Z., M. J. Federle, D. Marra, W. M. de Vos, O. P. Kuipers, M. Kleerebezem, and J. R. Scott. 1998. Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength. Appl. Environ. Microbiol. 64:2763-2769.
7. Eliason, J. L., and N. Sternberg. 1987. Characterization of the binding sites of c1 repressor of bacteriophage P1. Evidence for multiple asymmetric sites. J. Mol. Biol. 198:281-293.
8. Engel, H. W., N. Soedirman, J. A. Rost, W. J. van Leeuwen, and J. D. van Embden. 1980. Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J. Bacteriol. 142:407-413.
9. Friesenegger, A., S. Fiedler, L. A. Devriese, and R. Wirth. 1991. Genetic transformation of various species of Enterococcus by electroporation. FEMS Microbiol. Lett. 63:323-327.
10. Garvey, P., G. F. Fitzgerald, and C. Hill. 1995. Cloning and DNA sequence analysis of two abortive infection phage resistance determinants from the lactococcal plasmid pNP40. Appl. Environ. Microbiol. 61:4321-4328.
11. Geissendorfer, M., and W. Hillen. 1990. Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements. Appl. Microbiol. Biotechnol. 33:657-663.
12. Gibson, E. M., N. M. Chace, S. B. London, and J. London. 1979. Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. J. Bacteriol. 137:614-619.
13. Gonzalez, C. F., and B. S. Kunka. 1983. Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl. Environ. Microbiol. 46:81-89.
14. Graves, M. C., and J. C. Rabinowitz. 1986. In vivo and in vitro transcription of the Clostridium pasteurianum ferredoxin gene. Evidence for "extended" promoter elements in gram-positive organisms. J. Biol. Chem. 261:11409-11415.
15. BAD promoter. J. Bacteriol. 177:4121-4130.
16. Heinrich, J., H. D. Riedel, B. R. Baumstark, M. Kimura, and H. Schuster. 1989. The c1 repressor of bacteriophage P1 operator-repressor interaction of wild-type and mutant repressor proteins. Nucleic Acids Res. 17:7681-7692.
17. Heinrich, J., M. Velleman, and H. Schuster. 1995. The tripartite immunity system of phages P1 and P7. FEMS Microbiol. Rev. 17:121-126.
18. Heinzel, T., M. Velleman, and H. Schuster. 1989. ban operon of bacteriophage P1. Mutational analysis of the c1 repressor-controlled operator. J. Mol. Biol. 205:127-135.
19. Heinzel, T., M. Velleman, and H. Schuster. 1990. The c1 repressor inactivator protein coi of bacteriophage P1. Cloning and expression of coi and its interference with c1 repressor function. J. Biol. Chem. 265:17928-17934.
20. A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res. 23:2351-2360.
21. Henkin, T. M., and A. L. Sonenshein. 1987. Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis. Mol. Gen. Genet. 209:467-474.
22. Hiramatsu, K., H. Hanaki, T. Ino, K. Yabuta, T. Oguri, and F. C. Tenover. 1997. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J. Antimicrob. Chemother. 40:135-136.
23. Huycke, M. M., D. F. Sahm, and M. S. Gilmore. 1998. Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future. Emerg. Infect. Dis. 4:239-249.
24. Jensen, P. R., and K. Hammer. 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64:82-87.
25. Ji, Y., A. Marra, M. Rosenberg, and G. Woodnutt. 1999. Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection. J. Bacteriol. 181:6585-6590.
26. Jones, R. N., S. A. Marshall, M. A. Pfaller, W. W. Wilke, R. J. Hollis, M. E. Erwin, M. B. Edmond, and R. P. Wenzel. 1997. Nosocomial enterococcal bloodstream infections in the SCOPE program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. SCOPE Hospital Study Group. Diagn. Microbiol. Infect. Dis. 29:95-102.
27. Kim, L., A. Mogk, and W. Schumann. 1996. A xylose-inducible Bacillus subtilis integration vector and its application. Gene 181:71-76.
28. Kreikemeyer, B., and P. G. Jerlstrom. 1999. An Escherichia coli-Enterococcus faecalis shuttle vector as a tool for the construction of a group B Streptococcus heterologous mutant expressing the beta antigen (Bac) of the C protein complex. FEMS Microbiol. Lett. 180:255-262.
29. Lanzer, M., and H. Bujard. 1988. Promoters largely determine the efficiency of repressor action. Proc. Natl. Acad. Sci. USA 85:8973-8977.
30. Lee, G., C. Talkington, and J. Pero. 1980. Nucleotide sequence of a promoter recognized by Bacillus subtilis RNA polymerase. Mol. Gen. Genet. 180:57-65.
31. Lee, J. C. 1995. Electroporation protocols for microorganisms, p. 209-215. In J. A. Nickoloff (ed.), Methods in molecular biology, vol. 47. Humana Press, Inc., Totowa, N.J.
32. Leonhardt, H., and J. C. Alonso. 1988. Construction of a shuttle vector for inducible gene expression in Escherichia coli and Bacillus subtilis. J. Gen. Microbiol. 134:605-609.
33. 2 regulatory elements. Nucleic Acids Res. 25:1203-1210.
34. McIntyre, D. A., and S. K. Harlander. 1989. Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation. Appl. Environ. Microbiol. 55:604-610.
35. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
36. Moran, C. P., Jr., N. Lang, S. F. LeGrice, G. Lee, M. Stephens, A. L. Sonenshein, J. Pero, and R. Losick. 1982. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol. Gen. Genet. 186:339-346.
37. Powell, I. B., M. G. Achen, A. J. Hillier, and B. E. Davidson. 1988. A simple and rapid method for genetic transformation of lactic streptococci by electroporation. Appl. Environ. Microbiol. 54:655-660.
38. Poyart, C., and P. Trieu-Cuot. 1997. A broad-host-range mobilizable shuttle vector for the construction of transcriptional fusions to β-galactosidase in gram-positive bacteria. FEMS Microbiol. Lett. 156:193-198.
39. Rehm, S. J. 2002. Two new treatment options for infections due to drugresistant gram-positive cocci. Cleve. Clin. J. Med. 69:397-401, 405-413.
40. Rosner, J. L. 1972. Formation, induction, and curing of bacteriophage P1 lysogens. Virology 48:679-680.
41. Ruoff, K. L., L. de la Maza, M. J. Murtagh, J. D. Spargo, and M. J. Ferraro. 1990. Species identities of enterococci isolated from clinical specimens. J. Clin. Microbiol. 28:435-437.
42. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
43. Schaefer, T. S., and J. B. Hays. 1991. Bacteriophage P1 Bof protein is an indirect positive effector of transcription of the phage bac-1 ban gene in some circumstances and a direct negative effector in other circumstances. J. Bacteriol. 173:6469-6474.
44. Schofield, D. A., C. Westwater, J. W. Dolan, J. S. Norris, and M. G. Schmidt. 2002. Tight regulation and modulation via a C1-regulated promoter in Escherichia coli and Pseudomonas aeruginosa. Curr. Microbiol. 44:425-430.
45. Schofield, D. A., C. Westwater, J. W. Dolan, M. G. Schmidt, and J. S. Norris. 2001. Controlled expression in Klebsiella pneumoniae and Shigella fiexneri using a bacteriophage P1-derived C1-regulated promoter system. J. Bacteriol. 183:6947-6950.
46. Stark, M. J. 1987. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene 51:255-267.
47. Stieger, M., B. Wohlgensinger, M. Kamber, L. Rolf, and W. Keck. 1999. Integrational plasmids for the tetracycline-regulated expression of genes in Streptococcus pneumoniae. Gene 226:243-251.
48. Velleman, M., T. Heinzel, and H. Schuster. 1992. The Bof protein of bacteriophage P1 exerts its modulating function by formation of a ternary complex with operator DNA and C1 repressor. J. Biol. Chem. 267:12174-12181.
49. Velleman, M., M. Heirich, A. Gunther, and H. Schuster. 1990. A bacteriophage P1-encoded modulator protein affects the P1 c1 repression system. J. Biol. Chem. 265:18511-18517.
50. Voskuil, M. I., and G. H. Chambliss. 1998. The - 16 region of Bacillus subtilis and other gram-positive bacterial promoters. Nucleic Acids Res. 26:3584-3590.
51. Voskuil, M. I., K. Voepel, and G. H. Chambliss. 1995. The - 16 region, a vital sequence for the utilization of a promoter in Bacillus subtilis and Escherichia coli. Mol. Microbiol. 17:271-279.
52. Wieland, K. P., B. Wieland, and F. Gotz. 1995. A promoter-screening plasmid and xylose-inducible, glucose-repressible expression vectors for Staphylococcus carnosus. Gene 158:91-96.
53. Wirth, R., F. Y. An, and D. B. Clewell. 1986. Highly efficient protoplast transformation system for Streptococcusfaecalis and a new Escherichia coli-S. faecalis shuttle vector. J. Bacteriol. 165:831-836.
54. Wright, J. J., A. Kumar, and R. S. Hayward. 1992. Hypersymmetry in a transcriptional terminator of Escherichia coli confers increased efficiency as well as bidirectionality. EMBO J. 11:1957-1964.
55. Yarmolinsky, M. B., and N. Sternberg. 1988. Bacteriophage P1, p. 291-438. In R. Calendar (ed.), The bacteriophages, vol. 1. Plenum Publishing Corp., New York, N.Y.
56. Zhang, L., F. Fan, L. M. Palmer, M. A. Lonetto, C. Petit, L. L. Voelker, A. St John, B. Bankosky, M. Rosenberg, and D. McDevitt. 2000. Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action. Gene 255:297-305.