Two-plasmid vector system for independently controlled expression of green and red fluorescent fusion proteins in Staphylococcus aureus

Applied and Environmental Microbiology

Volumn 79, Issue 9, 2013, Pages 3133-3136

  Brzoska, Anthony J ^a   Firth, Neville ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DIVISIONS; FILAMENT FORMATION; FUSION PROTEINS; GREEN FLUORESCENT PROTEIN; RED FLUORESCENT; RED FLUORESCENT PROTEINS; STAPHYLOCOCCUS AUREUS; VECTOR SYSTEMS;

BACTERIA; CELL PROLIFERATION; DNA; FLUORESCENCE;

PROTEINS;

BACTERIUM; GENE EXPRESSION; PLASMID; PROTEIN;

COCCOIDEA; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEIN; CYTOSKELETON PROTEIN; FTSZ PROTEIN, BACTERIA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

CELL DIVISION; CYTOLOGY; CYTOSKELETON; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METABOLISM; PLASMID; PROMOTER REGION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; CELL DIVISION; CYTOSKELETAL PROTEINS; CYTOSKELETON; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS;

EID: 84876345641     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00144-13     Document Type: Article

References (25)

1. Kluytmans J, Struelens M. 2009. Meticillin resistant Staphylococcus aureus in the hospital. BMJ 338:b364. doi:10.1136/bmj.b364.
2. Lewis PJ. 2004. Bacterial subcellular architecture: recent advances and future prospects. Mol. Microbiol. 54:1135-1150.
3. Malone CL, Boles BR, Lauderdale KJ, Thoendel M, Kavanaugh JS, Horswill AR. 2009. Fluorescent reporters for Staphylococcus aureus. J. Microbiol. Methods 77:251-260.
4. Guo Y, Ramos RI, Cho JS, Donegan NP, Cheung AL, Miller LS. 2013. In vivo bioluminescence imaging to evaluate systemic and topical antibiotics against community-acquired methicillin-resistant Staphylococcus aureus-infected skin wounds in mice. Antimicrob. Agents Chemother. 57:855-863.
5. Boles BR, Horswill AR. 2008. Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog. 4:e1000052. doi:10.1371/journal.ppat.1000052.
6. Pereira PM, Veiga H, Jorge AM, Pinho MG. 2010. Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus. Appl. Environ. Microbiol. 76:4346-4353.
7. Liew AT, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, Lewis PJ, Harry EJ. 2011. A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus. Microbiology 157:666-676.
8. Sastalla I, Chim K, Cheung GY, Pomerantsev AP, Leppla SH. 2009. Codon-optimized fluorescent proteins designed for expression in low-GC gram-positive bacteria. Appl. Environ. Microbiol. 75:2099-2110.
9. Paprotka K, Giese B, Fraunholz MJ. 2010. Codon-improved fluorescent proteins in investigation of Staphylococcus aureus host pathogen interactions. J. Microbiol. Methods 83:82-86.
10. Firth N, Apisiridej S, Berg T, O'Rourke BA, Curnock S, Dyke KG, Skurray RA. 2000. Replication of staphylococcal multiresistance plasmids. J. Bacteriol. 182:2170-2178.
11. Janniere L, Bruand C, Ehrlich SD. 1990. Structurally stable Bacillus subtilis cloning vectors. 87:53-61.
12. Kreiswirth BN, Lofdahl S, Betley MJ, O'Reilly M, Schlievert PM, Bergdoll MS, Novick RP. 1983. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709-712.
13. Kwong SM, Skurray RA, Firth N. 2004. Staphylococcus aureus multiresistance plasmid pSK41: analysis of the replication region, initiator protein binding and antisense RNA regulation. Mol. Microbiol. 51:497-509.
14. Ma X, Ehrhardt DW, Margolin W. 1996. Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 93:12998-13003.
15. Wu LJ, Errington J. 2004. Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtilis. Cell 117: 915-925.
16. Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N. 2007. Segrosome structure revealed by a complex of ParR with centromere DNA. 450:1268-1271.
17. Popp D, Xu W, Narita A, Brzoska AJ, Skurray RA, Firth N, Ghoshdastider U, Maeda Y, Robinson RC, Schumacher MA. 2010. Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation. J. Biol. Chem. 285:10130-10140.
18. Clements MO, Watson SP, Foster SJ. 1999. Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity. J. Bacteriol. 181: 3898-3903.
19. Veiga H, Jorge AM, Pinho MG. 2011. Absence of nucleoid occlusion effector Noc impairs formation of orthogonal FtsZ rings during Staphylococcus aureus cell division. Mol. Microbiol. 80:1366-1380.
20. Erickson HP, Anderson DE, Osawa M. 2010. FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one. Microbiol. Mol. Biol. Rev. 74:504-528.
21. Moller-Jensen J, Jensen RB, Lowe J, Gerdes K. 2002. Prokaryotic DNA segregation by an actin-like filament. EMBO J. 21:3119-3127.
22. Becker E, Herrera NC, Gunderson FQ, Derman AI, Dance AL, Sims J, Larsen RA, Pogliano J. 2006. DNA segregation by the bacterial actin AlfA during Bacillus subtilis growth and development. EMBO J. 25:5919-5931.
23. Polka JK, Kollman JM, Agard DA, Mullins RD. 2009. The structure and assembly dynamics of plasmid actin AlfA imply a novel mechanism of DNA segregation. J. Bacteriol. 191:6219-6230.
24. Gerdes K, Howard M, Szardenings F. 2010. Pushing and pulling in prokaryotic DNA segregation. 141:927-942.
25. Derman AI, Becker EC, Truong BD, Fujioka A, Tucey TM, Erb ML, Patterson PC, Pogliano J. 2009. Phylogenetic analysis identifies many uncharacterized actin-like proteins (Alps) in bacteria: regulated polymerization, dynamic instability and treadmilling in Alp7A. Mol. Microbiol. 73:534-552.