Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system

Journal of Bioscience and Bioengineering

Volumn 118, Issue 5, 2014, Pages 554-557

  Kazuta, Yasuaki ^a   Matsuura, Tomoaki ^a,b   Ichihashi, Norikazu ^a   Yomo, Tetsuya ^a,b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Dialysis; Green fluorescent protein; In vitro protein synthesis; PURE system; Synthetic biology

Indexed keywords

BIOLOGY; DIALYSIS; ESCHERICHIA COLI; FLUORESCENCE; PROTEINS;

COMPONENT CONCENTRATION; DIHYDROFOLATE REDUCTASE; ESCHERICHIA COLI CELLS; GREEN FLUORESCENT PROTEIN; IN-VITRO; PROTEIN CONCENTRATIONS; PURE SYSTEM; SYNTHETIC BIOLOGY;

BIOSYNTHESIS;

BETA GALACTOSIDASE; DIHYDROFOLATE REDUCTASE; GREEN FLUORESCENT PROTEIN;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; DIALYSIS; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; BIOSYNTHESIS; DIRECTED MOLECULAR EVOLUTION; GENETICS; METABOLISM; PROCEDURES;

ESCHERICHIA COLI;

BETA-GALACTOSIDASE; DIALYSIS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; IN VITRO TECHNIQUES; PROTEIN BIOSYNTHESIS; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 84927174869     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2014.04.019     Document Type: Article

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