Effective and durable genetic modification of human mesenchymal stem cells via controlled release of rAAV vectors from self-assembling peptide hydrogels with a maintained differentiation potency

Acta Biomaterialia

Volumn 18, Issue , 2015, Pages 118-127

  Rey Rico, Ana ^a   Venkatesan, Jagadeesh K ^a   Frisch, Janina ^a   Schmitt, Gertrud ^a   Monge Marcet, Amália ^b   Lopez Chicon, Patricia ^c   Mata, Alvaro ^b   Semino, Carlos ^c   Madry, Henning ^a   Cucchiarini, Magali ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^c UNIVERSITAT RAMON LLULL   (Spain)

Author keywords

Differentiation potency; Human MSCs; rAAV gene transfer; Self assembling hydrogels

Indexed keywords

BIOCOMPATIBILITY; CELL CULTURE; GENE TRANSFER; GENES; HYALURONIC ACID; IONIC STRENGTH; PEPTIDES; PH; STEM CELLS; TISSUE ENGINEERING; VECTORS; VIRUSES;

ADENO-ASSOCIATED VIRUS VECTORS; DIFFERENTIATION POTENCY; HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS; HUMAN MSC; PEPTIDE HYDROGELS; RECOMBINANT ADENO-ASSOCIATED VIRUS; RECOMBINANT ADENO-ASSOCIATED VIRUS GENE TRANSFER; SELF-ASSEMBLING; SELF-ASSEMBLING HYDROGEL; SELF-ASSEMBLING PEPTIDES;

HYDROGELS;

AGGRECAN; COLLAGEN TYPE 1; COLLAGEN TYPE 10; COLLAGEN TYPE 2; HYALURONIC ACID; PARVOVIRUS VECTOR; PEPTIDE; SELF ASSEMBLING PEPTIDE; TOLONIUM CHLORIDE; TRANSCRIPTION FACTOR SOX9; UNCLASSIFIED DRUG; DELAYED RELEASE FORMULATION; HYDROGEL; RADA16-I;

ARTICLE; BIOLOGICAL ACTIVITY; BONE MARROW DERIVED MESENCHYMAL STEM CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL SURVIVAL; CELL VIABILITY; CHONDROGENESIS; CLINICAL ARTICLE; CYTOTOXICITY; DNA MODIFICATION; FLUORESCENCE; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; HYDROGEL; MONOLAYER CULTURE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TISSUE ENGINEERING; TRANSGENE; CYTOLOGY; DELAYED RELEASE FORMULATION; DEPENDOPARVOVIRUS; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC PROCEDURES; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STROMA CELL; METABOLISM; PHARMACOLOGY;

ADENO-ASSOCIATED VIRUS;

CELL DIFFERENTIATION; CELL SURVIVAL; CHONDROGENESIS; DELAYED-ACTION PREPARATIONS; DEPENDOVIRUS; GENE EXPRESSION REGULATION; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; HYALURONIC ACID; HYDROGELS; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STROMAL CELLS; PEPTIDES; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84927030976     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2015.02.013     Document Type: Article

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