Genomic DNA extraction from whole blood using a digital microfluidic (DMF) platform with magnetic beads

Microsystem Technologies

Volumn 23, Issue 2, 2017, Pages 313-320

  Hung, Ping Yi ^a   Jiang, Pei Shing ^b   Lee, Erh Fang ^b   Fan, Shih Kang ^a   Lu, Yen Wen ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; DIGITAL MICROFLUIDICS; DNA; ELECTROPHORESIS; NUCLEIC ACIDS; WASHING;

CENTRIFUGAL SEPARATION; EXTRACTION PROTOCOLS; GEL ELECTROPHORESIS; GENOMIC DNA EXTRACTION; MAGNETIC BEADS; MANUAL OPERATIONS; MICRO-FLUIDIC DEVICES; SAMPLE PREPARATION;

EXTRACTION;

EID: 84926352369     PISSN: 09467076     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00542-015-2512-9     Document Type: Article

References (20)

1. Boles DJ, Benton JL, Siew GJ, Levy MH, Thwa Prasanna K, Sandahl MA, Rouse JL, Perkins LC, Sudarsan AP, Jalili R, Pamula VK, Srinivasan V, Fair RB, P. B. G. ffi. n, Eckhardt AE, Pollack MG (2011) Droplet-based pyrosequencing using digital microfluidics. Anal Chem 83:8439–8447
2. Claassen S, du Toit E, Kaba M, Moodley C, Zar HJ, Nicol MP (2013) A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples. J Microbiol Methods 94:103–110
3. Claassena S, Toit ED, Kaba M, Moodley C, Zar HJ, Nicol MP (2013) A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples. J Microbiol Methods 94:103–110
4. Fan S-K, Wang F-M (2014) Multiphase optofluidics on an electro-microfluidic platform powered by electrowetting and dielectrophoresis. Lab Chip 14:2728–2738
5. Geng T, Bao N, Sriranganathanw N, Li L, Lu C (2012) Genomic DNA extraction from cells by electroporation on an integrated microfluidic platform. Anal Chem 84:9632–9639
6. Hilz H, Wiegers U, Adamietz P (1975) Stimulation of proteinase K action by denaturing agents application to the isolation of nucleic acids and the degradation of masked proteins. Eur J Biochem 56:103–108
7. Hsieh Y-L, Ho T-Y, Chakrabarty K (2014) Biochip synthesis and dynamic error recovery for sample preparation using digital microfluidics. IEEE Trans Comput Aided Des Integr Circuits Syst 33:183–196
8. Hua Z, Rouse JL, Eckhardt AE, Srinivasan V, Pamula VK, Schell WA, Benton JL, Mitchell TG, Pollack MG (2010) Multiplexed real-time polymerase Chain reaction on a digital microfluidic platform. Anal Chem 82:2310–2316
9. Hung P-Y, Lu H-Y, Fan S-K, Lu Y-W (2014) Mechatronic systems in digital microfluidics. Int J Autom Smart Technol 4:216–221
10. Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345:646–651
11. Jebrail MJ, Wheeler AR (2010) Let’s get digital: digitizing chemical biology with microfluidics. Curr Opin Chem Biol 14:574–581
12. Jebrail MJ, Bartsch MS, Patel KD (2012) Digital microfluidics: a versatile tool for applications in chemistry, biology and medicine. Lab Chip 12:2452–2463
13. Jebrail MJ, Sinha A, Vellucci S, Renzi RF, Cesar Ambriz C, Gondhalekar JS Schoeniger, Patel Kamlesh D, Branda SS (2014) World-to-digital-microfluidic interface enabling extraction and purification of RNA from human whole blood. Anal Chem 86:3856–3862
14. Kim J, Johnson M, Hill P, Sonkul RS, Kim J, Gale BK (2012) Automated microfluidic DNA/RNA extraction with both disposable and reusable components. J Micromech Microeng 22:015007
15. Kim H, Jebrail MJ, Sinha A, Bent ZW, Solberg OD, Williams KP, Langevin SA, Renzi RF, Van De Vreugde JL, Meagher RJ, Schoeniger JS, Lane TW, Branda SS, Bartsch MS, Patel KD (2013) A microfluidic DNA library preparation platform for next-generation sequencing. Plos One 8:e68988
16. Mousa NA, Jebrail MJ, Yang H, Abdelgawad M, Metalnikov P, Chen J, Wheeler AR, Casper RF (2009) Droplet-scale estrogen assays in breast tissue, Blood, and Serum. Sci Transl Med 1:1ra2
17. Rival A, Jary D, Delattre C, Fouillet Y, Castellan G, Bellemin-Comte A, Gidrol X (2014) An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR. Lab Chip 14(19):3739–3749
18. Shamsi MH, Choi K, Ng AHC, Wheeler AR (2014) A digital microfluidic electrochemical immunoassay. Lab Chip 14:547–554
19. Shen H-H, Fan S-K, Kim C-J, Yao D-J (2014) EWOD microfluidic systems for biomedical applications. Microfluid Nanofluid 16:965–987
20. Sista R, Hua Z, Thwar P, Sudarsan A, Srinivasan V, Eckhardt A, Pollack M, Pamula V (2008) Development of a digital microfluidic platform for point of care testing. Lab Chip 8:2091–2104