Co-expression of functional human Heme Oxygenase 1, Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by "self-cleaving" 2A peptide system

Plasmid

Volumn 79, Issue , 2015, Pages 22-29

  De Giorgi, Marco ^a,b   Cinti, Alessandro ^a   Pelikant Malecka, Iwona ^b   Chisci, Elisa ^a   Lavitrano, Marialuisa ^a   Giovannoni, Roberto ^a   Smolenski, Ryszard T ^a,b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b MEDICAL UNIVERSITY OF GDANSK   (Poland)

Author keywords

2A peptide; Ecto 5' nucleotidase; Ecto nucleoside triphosphate diphosphohydrolase 1; Heme oxygenase 1; Multicistronic vector; Xenotransplantation

Indexed keywords

ECTO 5' NUCLEOTIDASE; ECTO 5' NUCLEOTIDASE PLUS ECTO NUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 1 PLUS HEME OXYGENASE 1; ECTO NUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 1; FURIN; GENE THERAPY AGENT; HEME OXYGENASE 1; NUCLEOSIDE TRIPHOSPHATE; UNCLASSIFIED DRUG; 5' NUCLEOTIDASE; APYRASE; CD39 ANTIGEN; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; HMOX1 PROTEIN, HUMAN; LEUKOCYTE ANTIGEN; NT5E PROTEIN, HUMAN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENETIC TRANSFECTION; HEK293 CELL LINE; HUMAN; PLASMID; PLASMID P2A PEPTIDE SYSTEM; PLASMID PFUR2A PEPTIDE SYSTEM; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; TRICISTRONIC PLASMID; VIRAL GENE DELIVERY SYSTEM; VIRAL GENE THERAPY; XENOTRANSPLANTATION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; XENOGRAFT;

5'-NUCLEOTIDASE; ANTIGENS, CD; APYRASE; FURIN; GENE EXPRESSION REGULATION; GPI-LINKED PROTEINS; HEK293 CELLS; HEME OXYGENASE-1; HUMANS; PLASMIDS; TRANSPLANTATION, HETEROLOGOUS;

EID: 84926051660     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2015.03.004     Document Type: Article

References (37)

1. Akamatsu Y., Haga M., Tyagi S., Yamashita K., Graça-Souza A.V., Ollinger R., et al. Heme oxygenase-1-derived carbon monoxide protects hearts from transplant associated ischemia reperfusion injury. FASEB J. 2004, 18:771-772.
2. Antonioli L., Pacher P., Vizi E.S., Haskó G. CD39 and CD73 in immunity and inflammation. Trends Mol. Med 2013, 19:355-367.
3. Arber C., Abhyankar H., Heslop H.E., Brenner M.K., Liu H., Dotti G., et al. The immunogenicity of virus-derived 2A sequences in immunocompetent individuals. Gene Ther 2013, 20:958-962.
4. Cooper D.K.C., Ekser B., Burlak C., Ezzelarab M., Hara H., Paris L., et al. Clinical lung xenotransplantation-what donor genetic modifications may be necessary?. Xenotransplantation 2012, 19:144-158.
5. Cooper D.K.C., Hara H., Ezzelarab M., Bottino R., Trucco M., Phelps C., et al. The potential of genetically-engineered pigs in providing an alternative source of organs and cells for transplantation. J. Biomed. Res 2013, 27:249-253.
6. Cozzi E., Tucker A.W., Langford G.A., Pino-Chavez G., Wright L., O'Connell M.J., et al. Characterization of pigs transgenic for human decay-accelerating factor. Transplantation 1997, 64:1383-1392.
7. de Felipe P., Ryan M.D. Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences. Traffic 2004, 5:616-626.
8. de Felipe P., Martín V., Cortés M.L., Ryan M., Izquierdo M. Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy. Gene Ther 1999, 6:198-208.
9. de Felipe P., Luke G.A., Hughes L.E., Gani D., Halpin C., Ryan M.D. E unum pluribus: multiple proteins from a self-processing polyprotein. Trends Biotechnol 2006, 24:68-75.
10. De Giorgi M., Pelikant-Malecka I., Sielicka A., Slominska E.M., Giovannoni R., Cinti A., et al. Functional analysis of expression of human ecto-nucleoside triphosphate diphosphohydrolase-1 and/or ecto-5'-nucleotidase in pig endothelial cells. Nucleosides Nucleotides Nucleic Acids 2014, 33:313-318.
11. Deng W., Yang D., Zhao B., Ouyang Z., Song J., Fan N., et al. Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS ONE 2011, 6:e19986.
12. Di Stasi A., Tey S.K., Dotti G., Fujita Y., Kennedy-Nasser A., Martinez C., et al. Inducible apoptosis as a safety switch for adoptive cell therapy. N. Engl. J. Med 2011, 365:1673-1683.
13. Dwyer K.M., Robson S.C., Nandurkar H.H., Campbell D.J., Gock H., Murray-Segal L.J., et al. Thromboregulatory manifestations in human CD39 transgenic mice and the implications for thrombotic disease and transplantation. J. Clin. Invest 2004, 113:1440-1446.
14. Fang J., Qian J.-J., Yi S., Harding T.C., Tu G.H., VanRoey M., et al. Stable antibody expression at therapeutic levels using the 2A peptide. Nat. Biotechnol 2005, 23:584-590.
15. Fisicaro N., Londrigan S.L., Brady J.L., Salvaris E., Nottle M.B., O'Connell P.J., et al. Versatile co-expression of graft-protective proteins using 2A-linked cassettes. Xenotransplantation 2011, 18:121-130.
16. Gock H., Nottle M., Lew A.M., d'Apice A.J.F., Cowan P. Genetic modification of pigs for solid organ xenotransplantation. Transplant. Rev. (Orlando) 2011, 25:9-20.
17. Gottlieb Y., Truman M., Cohen L.A., Leichtmann-Bardoogo Y., Meyron-Holtz E.G. Endoplasmic reticulum anchored heme-oxygenase 1 faces the cytosol. Haematologica 2012, 97:1489-1493.
18. Hasegawa T., Bouïs D., Liao H., Visovatti S.H., Pinsky D.J. Ecto-5' nucleotidase (CD73)-mediated adenosine generation and signaling in murine cardiac allograft vasculopathy. Circ. Res 2008, 103:1410-1421.
19. Huttinger Z.M., Milks M.W., Nickoli M.S., Aurand W.L., Long L.C., Wheeler D.G., et al. Ectonucleotide triphosphate diphosphohydrolase-1 (CD39) mediates resistance to occlusive arterial thrombus formation after vascular injury in mice. Am. J. Pathol 2012, 181:322-333.
20. Kaczmarek E., Koziak K., Sévigny J., Siegel J.B., Anrather J., Beaudoin A.R., et al. Identification and characterization of CD39/vascular ATP diphosphohydrolase. J. Biol. Chem 1996, 271:33116-33122.
21. Klemens M.R., Sherman W.R., Holmberg N.S., Ruedi S.M., Low M.G., Thompson L.F. Characterization of soluble vs membrane-bound human placental 5'-nucleotidase. Biochem. Biophys. Res. Commun 1990, 172:1371-1377.
22. Klemz R., Mashreghi M.-F., Spies C., Volk H.-D., Kotsch K. Amplifying the fluorescence of bilirubin enables the real-time detection of heme oxygenase activity. Free Radic. Biol. Med 2009, 46:305-311.
23. Lavitrano M., Bacci M.L., Forni M., Lazzereschi D., Di Stefano C., Fioretti D., et al. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proc. Natl. Acad. Sci. U.S.A. 2002, 99:14230-14235.
24. Mansouri M., Berger P. Strategies for multigene expression in eukaryotic cells. Plasmid 2014, 75:12-17.
25. Minskaia E., Nicholson J., Ryan M.D. Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications. BMC Biotechnol 2013, 13:67.
26. Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y. Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form. Eur. J. Biochem 1990, 191:563-569.
27. Osborne F.N., Kalsi K.K., Lawson C., Lavitrano M., Yacoub M.H., Rose M.L., et al. Expression of human ecto-5'-nucleotidase in pig endothelium increases adenosine production and protects from NK cell-mediated lysis. Am. J. Transplant 2005, 5:1248-1255.
28. Park S.J., Cho B., Koo O.J., Kim H., Kang J.T., Hurh S., et al. Production and characterization of soluble human TNFRI-Fc and human HO-1 (HMOX1) transgenic pigs by using the F2A peptide. Transgenic Res 2014, 23:407-419.
29. Petersen B., Ramackers W., Lucas-Hahn A., Lemme E., Hassel P., Queisser A.-L., et al. Transgenic expression of human heme oxygenase-1 in pigs confers resistance against xenograft rejection during ex vivo perfusion of porcine kidneys. Xenotransplantation 2011, 18:355-368.
30. Rothwell D.G., Crossley R., Bridgeman J.S., Sheard V. Functional expression of secreted proteins from a bicistronic retroviral cassette based on FMDV 2A can be position- dependent. Hum. Gene Ther 2010, 21:1631-1637.
31. Smolenski R.T., Lachno D.R., Ledingham S.J., Yacoub M.H. Determination of sixteen nucleotides, nucleosides and bases using high-performance liquid chromatography and its application to the study of purine metabolism in hearts for transplantation. J. Chromatogr 1990, 527:414-420.
32. Soares M.P., Bach F.H. Heme oxygenase-1: from biology to therapeutic potential. Trends Mol. Med 2009, 15:50-58.
33. Soares M.P., Seldon M.P., Gregoire I.P., Vassilevskaia T., Berberat P.O., Yu J., et al. Heme oxygenase-1 modulates the expression of adhesion molecules associated with endothelial cell activation. J. Immunol 2004, 172:3553-3563.
34. Verrier J.D., Madorsky I., Coggin W.E., Geesey M., Hochman M., Walling E., et al. Bicistronic lentiviruses containing a viral 2A cleavage sequence reliably co-express two proteins and restore vision to an animal model of LCA1. PLoS ONE 2011, 6:e20553.
35. Yang S., Cohen C.J., Peng P.D., Zhao Y., Cassard L., Yu Z., et al. Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition. Gene Ther 2008, 15:1411-1423.
36. Yin W., Xiang P., Li Q. Investigations of the effect of DNA size in transient transfection assay using dual luciferase system. Anal. Biochem 2005, 346:289-294.
37. Zernecke A., Bidzhekov K., Ozüyaman B., Fraemohs L., Liehn E.A., Lüscher-Firzlaff J.M., et al. CD73/ecto-5'-nucleotidase protects against vascular inflammation and neointima formation. Circulation 2006, 113:2120-2127.