A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation

Bioscience, Biotechnology and Biochemistry

Volumn 78, Issue 5, 2014, Pages 832-838

  Mori, Tadashi ^a   Hidaka, Masafumi ^a   Ikuji, Hiroko ^a   Yoshizawa, Ibuki ^a   Toyohara, Haruhiko ^b   Okuda, Toru ^c   Uchida, Chiyoko ^d   Asano, Tomoichiro ^e   Yotsu Yamashita, Mari ^a   Uchida, Takafumi ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c TAMAGAWA UNIVERSITY   (Japan)

^d FUKUSHIMA UNIVERSITY   (Japan)

^e HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Adipose cell; High throughput screen; Obesity; Prolyl isomerase; Seaweed polyphenol

Indexed keywords

CELL CULTURE; CELLS; CYTOLOGY; FIBROBLASTS; NUTRITION; SEAWEED;

ADIPOSE CELLS; HIGH-THROUGHPUT SCREENS; OBESITY; POLYPHENOLS; PROLYL ISOMERASE;

THROUGHPUT;

ECKLONIA KUROME;

BIOLOGICAL PRODUCT; ENZYME INHIBITOR; NIMA-INTERACTING PEPTIDYLPROLYL ISOMERASE; PEPTIDYLPROLYL ISOMERASE; POLYPHENOL;

3T3 CELL LINE; ADIPOCYTE; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL DIFFERENTIATION; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; DRUG SCREENING; FIBROBLAST; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; MOUSE; SEAWEED;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; BIOLOGICAL PRODUCTS; CELL DIFFERENTIATION; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; FIBROBLASTS; HIGH-THROUGHPUT SCREENING ASSAYS; MICE; PEPTIDYLPROLYL ISOMERASE; POLYPHENOLS; SEAWEED;

EID: 84925884153     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1080/09168451.2014.905189     Document Type: Article

References (22)

1. Takahashi K, Uchida C, Shin RW, Shimazaki K, Uchida T. Prolyl isomerase Pin1: New findings on post-translational modifications and physiological substrates in cancer, Alzheimer's disease and asthma. Cell. Mol. Life Sci. 2008;65:359-375.
2. Uchida T, Furumai K, Fukuda T, Akiyama H, Takezawa M, Asano T, Fujimori F, Uchida C. Prolyl Isomerase Pin1 Regulates Mouse Embryonic Fibroblast Differentiation into Adipose Cells. PLoS ONE. 2012;7:e31823.
3. Nakatsu Y, Otani Y, Sakoda H, Zhang J, Guo Y, Okubo H, Kushiyama A, Fujishiro M, Kikuchi T, Fukushima T, Ohno H, Tsuchiya Y, Kamata H, Nagamachi A, Inaba T, Nishimura F, Katagiri H, Takahashi SI, Kurihara H, Uchida T, Asano T. Role of Pin1 in the pathogenesis of non-alcoholic steatohepatitis in a rodent model. J. Biol. Chem. 2012;287:44526-44535.
4. Nakatsu Y, Sakoda H, Kushiyama A, Zhang J, Ono H, Fujishiro M, Kikuchi T, Fukushima T, Yoneda M, Ohno H, Horike N, Kanna M, Tsuchiya Y, Kamata H, Nishimura F, Isobe T, Ogihara T, Katagiri H, Oka Y, Takahashi SI, Kurihara H, Uchida T, Asano T. Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 associates with IRS-1 and enhances insulin actions and adipogenesis. J. Biol. Chem. 2011;286:20812-20822.
5. Nakatsu Y, Sakoda H, Kushiyama A, Ono H, Fujishiro M, Horike N, Yoneda M, Ohno H, Kamata H, Nishimura F, Ogihara T, Katagiri H, Oka Y, Fukushima T, Takahashi S, Kurihara H, Uchida T, Asano T. Pin1 associates with and induces translocation of CRTC2 to the cytosol, thereby suppressing CRE transcriptional activity. J. Biol. Chem. 2010;285:33018-33027.
6. Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nat. Rev. Drug Discovery 2005;4:206-220.
7. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 2007;70:461-477.
8. Clardy J, Walsh C. Lessons from natural molecules. Nature. 2004;432:829-837.
9. Sedrani R, Kallen J, Martin Cabrejas LM, Papageorgiou CD, Senia F, Rohrbach S, Wagner D, Thai B, Jutzi Eme AM, France J, Oberer L, Rihs G, Zenke G, Wagner J. Sanglifehrin-Cyclophilin Interaction: Degradation Work, Synthetic Macrocyclic Analogues, X-ray Crystal Structure, and Binding Data. J. Am. Chem. Soc. 2003;125:3849-3859.
10. Uchida T, Takamiya M, Takahashi M, Miyashita H, Ikeda H, Rohrbach S, Wagner D, Thai B, Jutzi Eme AM, France J, Oberer L, Rihs G, Zenke G, Wagner J. Pin1 and Par14 peptidyl prolyl isomerase inhibitors block cell proliferation. Chem. Biol. 2003;10:15-24.
11. Tatara Y, Lin YC, Bamba Y, Mori T, Uchida T. Dipentamethylene thiuram monosulfide is a novel inhibitor of Pin1. Biochem. Biophys. Res. Commun. 2009;384:394-398.
12. Mori T, Itami S, Yanagi T, Tatara Y, Takamiya M, Uchida T. Use of a Real-Time Fluorescence Monitoring System for High-Throughput Screening for Prolyl Isomerase Inhibitors. J. Biomol. Screening 2009;14:419-424.
13. Mori T, Hidaka M, Lin YC, Yoshizawa I, Okabe T, Egashira S, Kojima H, Nagano T, Koketsu M, Takamiya M, Uchida T. A dual inhibitor against prolyl isomerase Pin1 and cyclophilin discovered by a novel real-time fluorescence detection method. Biochem. Biophys. Res. Commun. 2011;406:439-443.
14. Zhang JH, Chung TD, Oldenburg KR. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J. Biomol. Screening 1999;4:67-73.
15. Fischer G, Bang H, Berger E, Schellenberger A. Conformational specificity of chymotrypsin toward proline-containing substrates. Biochim. Biophys. Acta 1984;791:87-97.
16. Yotsu-Yamashita M, Kondo S, Segawa S, Lin YC, Toyohara H, Ito H, Konoki K, Cho Y, Uchida T. Isolation and structural determination of two novel phlorotannins from the brown alga Ecklonia kurome Okanura, and their radical scavenging activities. Mar. Drugs 2013;11:165-183.
17. James LK, Petr K, Vimal K, Esther CB, Daniel HR. Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay. Biochemistry 1991;30:6127-6134.
18. Subramanium V, Ayyavu M, Balaji ND, Nattarayan K. Mosquito larvicidal efficacy of phenolic acids of seaweed Chaetomorpha antennina (Bory) Kuetz. against Aedes aegypti. J. Appl. Phycol. 2004;67:212-216.
19. Albertus JS. Medicinal and pharmaceutical uses of seaweed natural products: A review. J. Appl. Phycol. 2004;16:245-262.
20. Shibata T, Kawaguchi S, Hama Y, Inagaki M, Yamaguchi K, Nakamura T. Local and chemical distribution of phlorotannins in brown algae. J. Appl. Phycol. 2004;16:291-296.
21. Nakamura T, Nagayama K, Uchida K, Tanaka R. Antioxidant activity of phlorotannins isolated from the Brown Alga Eisenia Bicyclis. Fish. Sci. 1996;62:923-926.
22. Xu HL, Kitajima C, Ito H, Miyazaki T, Baba M, Okuyama T, Okada Y. Antidiabetic effect of polyphenols from brown alga Ecklonia kurome in genetically diabetic KK-A(y) mice. Pharm. Biol. 2012;50:393-400.