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Volumn 39, Issue SUPPL. 2, 2011, Pages

PHUSER (Primer Help for USER): A novel tool for USER fusion primer design

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; POLYKETIDE SYNTHASE;

EID: 79959930123     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkr394     Document Type: Article
Times cited : (16)

References (15)
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    • Precise gene fusion by PCR
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    • (1989) Nucleic Acids Research , vol.17 , Issue.12 , pp. 4895
    • Yon, Y.1    Fried, M.2
  • 2
    • 0030638485 scopus 로고    scopus 로고
    • Creation of chimeric junctions deletions, and insertions by PCR
    • Pont-Kingdon, G. (1997) Creation of chimeric junctions, deletions, and insertions by PCR. Methods Mol. Biol., 67, 167-172.
    • (1997) Methods Mol. Biol. , vol.67 , pp. 167-172
    • Pont-Kingdon, G.1
  • 3
    • 0037082166 scopus 로고    scopus 로고
    • PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors
    • Kuwayama, H., Obara, S., Morio, T., Katoh, M., Urushihara, H. and Tanaka, Y. (2002) PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acids Res., 30, E2.
    • (2002) Nucleic Acids Res. , vol.30
    • Kuwayama, H.1    Obara, S.2    Morio, T.3    Katoh, M.4    Urushihara, H.5    Tanaka, Y.6
  • 4
    • 34249854159 scopus 로고    scopus 로고
    • USER fusion: A rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    • Geu-Flores, F., Nour-Eldin, H.H., Nielsen, M.T. and Halkier, B.A. (2007) USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products. Nucleic Acids Res., 35, e55.
    • (2007) Nucleic Acids Res. , vol.35
    • Geu-Flores, F.1    Nour-Eldin, H.H.2    Nielsen, M.T.3    Halkier, B.A.4
  • 5
    • 77956941760 scopus 로고    scopus 로고
    • USER cloning and USER fusion: The ideal cloning techniques for small and big laboratories
    • Nour-Eldin, H.H., Geu-Flores, F. and Halkier, B.A. (2010) USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories. Methods Mol. Biol., 643, 185-200.
    • (2010) Methods Mol. Biol. , vol.643 , pp. 185-200
    • Nour-Eldin, H.H.1    Geu-Flores, F.2    Halkier, B.A.3
  • 8
    • 33646270475 scopus 로고    scopus 로고
    • PCR primer design
    • Dieffenbach, C.W. and Dveksler, G.S. (eds) 2 edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA
    • Apte, A. and Saurabha, D. (2003) PCR primer design. In Dieffenbach, C.W. and Dveksler, G.S. (eds), PCR Primer a Laboratory Manual, 2 edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
    • (2003) PCR Primer A Laboratory Manual
    • Apte, A.1    Saurabha, D.2
  • 10
  • 12
    • 79955587715 scopus 로고    scopus 로고
    • Versatile enzyme expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the mycophenolic acid gene cluster as a test case
    • Hansen, B.G., Salomonsen, B., Nielsen, M.T., Nielsen, J.B., Hansen, N.B., Nielsen, K.F., Regueira, T.B., Nielsen, J., Patil, K.R. and Mortensen, U.H. (2011) Versatile enzyme expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the mycophenolic acid gene cluster as a test case. Appl. Environ. Microbiol., 77(9), 3044-3051.
    • (2011) Appl. Environ. Microbiol. , vol.77 , Issue.9 , pp. 3044-3051
    • Hansen, B.G.1    Salomonsen, B.2    Nielsen, M.T.3    Nielsen, J.B.4    Hansen, N.B.5    Nielsen, K.F.6    Regueira, T.B.7    Nielsen, J.8    Patil, K.R.9    Mortensen, U.H.10
  • 13
    • 73849104591 scopus 로고    scopus 로고
    • USER friendly DNA recombination (USERec): A simple and flexible near homology-independent method for gene library construction
    • Villiers, B.R., Stein, V. and Hollfelder, F. (2010) USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction. Protein Eng. Des. Sel., 23, 1-8.
    • (2010) Protein Eng. Des. Sel. , vol.23 , pp. 1-8
    • Villiers, B.R.1    Stein, V.2    Hollfelder, F.3
  • 14
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    • An efficient method to assemble linear DNA templates for in vitro screening and selection systems
    • Stein, V. and Hollfelder, F. (2009) An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res., 37, e122.
    • (2009) Nucleic Acids Res. , vol.37
    • Stein, V.1    Hollfelder, F.2
  • 15
    • 57649116988 scopus 로고    scopus 로고
    • Towards engineering glucosinolates into non-cruciferous plants
    • Geu-Flores, F., Olsen, C.E. and Halkier, B.A. (2009) Towards engineering glucosinolates into non-cruciferous plants. Planta, 229, 261-270.
    • (2009) Planta , vol.229 , pp. 261-270
    • Geu-Flores, F.1    Olsen, C.E.2    Halkier, B.A.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.