Purification and characterization of a cold-adapted pullulanase from a psychrophilic bacterial isolate

Extremophiles

Volumn 18, Issue 6, 2014, Pages 1095-1102

  Qoura, Farah ^a,b   Elleuche, Skander ^a   Brueck, Thomas ^b   Antranikian, Garabed ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Application; Biochemical characterisation; Cold adaptation; Enzymes; Psychrophiles

Indexed keywords

BACTERIAL PROTEIN; GLYCOSIDASE; PULLULANASE;

ADAPTATION; AMINO ACID SEQUENCE; ARCTIC; CHEMISTRY; COLD; ENZYMOLOGY; METABOLISM; MOLECULAR GENETICS; SHEWANELLA;

ADAPTATION, PHYSIOLOGICAL; AMINO ACID SEQUENCE; ARCTIC REGIONS; BACTERIAL PROTEINS; COLD TEMPERATURE; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; SHEWANELLA;

EID: 84925624002     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-014-0678-1     Document Type: Article

References (41)

1. Adams KR, Priest FG (1977) Extracellular pullulanase synthesis in Bacillus macerans. FEMS Microbiol Lett 1:269–273
2. Al Khudary R, Venkatachalam R, Katzer M, Elleuche S, Antranikian G (2010) A cold-adapted esterase of a novel marine isolate, Pseudoalteromonas arctica: gene cloning, enzyme purification and characterization. Extremophiles 14:273–285
3. Albertson GD, McHale RH, Gibbs MD, Bergquist PL (1997) Cloning and sequence of a type I pullulanase from an extremely thermophilic anaerobic bacterium, Caldicellulosiruptor saccharolyticus. Biochim Biophys Acta 1354:35–39
4. Antranikian G, Egorova K (2007) Extremophiles, a unique source of biocatalysts for industrial biotechnology. In: Gerday C, Glansdorff N (eds) Physiology and biochemistry of extremophiles. ASM Press, Wahington DC, pp 361–406
5. Bernfeld P (1955) Amylase a and b. Method Enzymol 1:149–155
6. Bertoldo C, Antranikian G (2002) Starch-hydrolyzing enzymes from thermophilic archaea and bacteria. Curr Opin Chem Biol 6:151–160
7. Bertoldo C, Duffner F, Jorgensen PL, Antranikian G (1999) Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme. Appl Environ Microbiol 65:2084–2091
8. Bertoldo C, Armbrecht M, Becker F, Schafer T, Antranikian G, Liebl W (2004) Cloning, sequencing, and characterization of a heat- and alkali-stable type I pullulanase from Anaerobranca gottschalkii. Appl Environ Microbiol 70:3407–3416
9. Bibel M, Brettl C, Gosslar U, Kriegshauser G, Liebl W (1998) Isolation and analysis of genes for amylolytic enzymes of the hyperthermophilic bacterium Thermotoga maritima. FEMS Microbiol Lett 158:9–15
10. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
11. Davids T, Schmidt M, Bottcher D, Bornscheuer UT (2013) Strategies for the discovery and engineering of enzymes for biocatalysis. Curr Opin Chem Biol 17:215–220
12. Duffner F, Bertoldo C, Andersen JT, Wagner K, Antranikian G (2000) A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis. J Bacteriol 182:6331–6338
13. Elleuche S, Antranikian G (2013) Starch-hydrolyzing enzymes from thermophiles. In: Satyanarayana T, Littlechild J, Kawarabayasi Y (eds) Thermophilic microbes in environmental and industrial biotechnology: biotechnology of thermophiles. Springer, Dordrecht, pp 509–533
14. Elleuche S, Piascheck H, Antranikian G (2011) Fusion of the OsmC domain from esterase EstO confers thermolability to the cold-active xylanase Xyn8 from Pseudoalteromonas arctica. Extremophiles 15:311–317
15. Groudieva T, Grote R, Antranikian G (2003) Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int J Syst Evol Microbiol 53:539–545
16. Groudieva T, Kambourova M, Yusef H, Royter M, Grote R, Trinks H, Antranikian G (2004) Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen. Extremophiles 8:475–488
17. Kang J, Park KM, Choi KH, Park CS, Kim GE, Kim D, Cha J (2011) Molecular cloning and biochemical characterization of a heat-stable type I pullulanase from Thermotoga neapolitana. Enzyme Microb Technol 48:260–266
18. Kelly AP, Diderichsen B, Jorgensen S, McConnell DJ (1994) Molecular genetic analysis of the pullulanase B gene of Bacillus acidopullulyticus. FEMS Microbiol Lett 115:97–105
19. Kim CH, Nashiru O, Ko JH (1996) Purification and biochemical characterization of pullulanase type I from Thermus caldophilus GK-24. FEMS Microbiol Lett 138:147–152
20. Kim HJ, Park S, Lee JM, Jung W, Kang JS, Joo HM, Seo KW, Kang SH (2008) Moritella dasanensis sp. nov., a psychrophilic bacterium isolated from the Arctic ocean. Int J Syst Evol Microbiol 58:817–820
21. Koivula TT, Hemila H, Pakkanen R, Sibakov M, Palva I (1993) Cloning and sequencing of a gene encoding acidophilic amylase from Bacillus acidocaldarius. J Gen Microbiol 139:2399–2407
22. Konneke M, Kuever J, Galushko A, Jorgensen BB (2013) Desulfoconvexum algidum gen. nov., sp. nov., a psychrophilic sulfate-reducing bacterium isolated from a permanently cold marine sediment. Int J Syst Evol Microbiol 63:959–964
23. Kornacker MG, Pugsley AP (1990) Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023. Mol Microbiol 4:73–85
24. Kriegshauser G, Liebl W (2000) Pullulanase from the hyperthermophilic bacterium Thermotoga maritima: purification by beta-cyclodextrin affinity chromatography. J Chromatogr B Biomed Sci Appl 737:245–251
25. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
26. Leiros HK, Willassen NP, Smalas AO (2000) Structural comparison of psychrophilic and mesophilic trypsins. Elucidating the molecular basis of cold-adaptation. Eur J Biochem 267:1039–1049
27. Malle D, Itoh T, Hashimoto W, Murata K, Utsumi S, Mikami B (2006) Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168. Acta Crystallogr Sect F Struct Biol Cryst Commun 62:381–384
28. Messoud BE, Ammar YB, Mellouli L, Bejar S (2002) Thermostable pullulanase typ I from new isolated Bacillus thermoleovorans US105: cloning, sequencing and expression of the gene in E. coli. Enzyme Microbiol Technol 31:827–832
29. Michaelis S, Chapon C, D’Enfert C, Pugsley AP, Schwartz M (1985) Characterization and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae. J Bacteriol 164:633–638
30. Niehaus F, Peters A, Groudieva T, Antranikian G (2000) Cloning, expression and biochemical characterisation of a unique thermostable pullulan-hydrolysing enzyme from the hyperthermophilic archaeon Thermococcus aggregans. FEMS Microbiol Lett 190:223–229
31. Nisha M, Satyanarayana T (2013a) Characterization of recombinant amylopullulanase (gt-apu) and truncated amylopullulanase (gt-apuT) of the extreme thermophile Geobacillus thermoleovorans NP33 and their action in starch saccharification. Appl Microbiol Biotechnol 97:6279–6292
32. Nisha M, Satyanarayana T (2013b) Recombinant bacterial amylopullulanases: developments and perspectives. Bioengineered 4(6):388–400
33. Odibo FJC, Obi SKC (1988) Purification and characterization of a thermostable pullulanase from Thermoactinomyces thalpophilus. J Ind Micro 3:343–350
34. Qoura F, Brueck T, Antranikian G (2014) The psychrophile Shewanella arctica sp. nov: a new source of industrially important enzyme systems. JSM Biotechnol. Biomed Eng 2:1027
35. Rajaei S, Heidari R, Shahbani Zahiri H, Sharifzadeh S, Torktaz I, Akbari Noghabi K (2013) A novel cold-adapted pullulanase from Exiguobacterium sp. SH3: production optimization, purification, and characterization. Starch/Stärke 65:1–10
36. Rüdiger A, Jorgensen PL, Antranikian G (1995) Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli. Appl Environ Microbiol 61:567–575
37. Srinivas TN, Nageswara Rao SS, Vishnu Vardhan Reddy P, Pratibha MS, Sailaja B, Kavya B, Hara Kishore K, Begum Z, Singh SM, Shivaji S (2009) Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of and Ny-alesund, Svalbard, Arctic. Curr Microbiol 59:537–547
38. Stefanova ME, Schwerdtfeger R, Antranikian G, Scandurra R (1999) Heat-stable pullulanase from Bacillus acidopullulyticus: characterization and refolding after guanidinium chloride-induced unfolding. Extremophiles 3:147–152
39. Suzuki Y, Hatagaki K, Oda H (1991) A hyperthermostable pullulanase produced by an extreme thermophile, Bacillus flavocaldarius KP 1228, and evidence for the proline theory of increasing protein thermostability. Appl Microbiol Biotechnol 34:707–714
40. Trincone A (2011) Marine biocatalysts: enzymatic features and applications. Mar Drugs 9:478–499
41. Zouari Ayadi D, Ben Ali M, Jemli S, Ben Mabrouk S, Mezghani M, Ben Messaoud E, Bejar S (2008) Heterologous expression, secretion and characterization of the Geobacillus thermoleovorans US105 type I pullulanase. Appl Microbiol Biotechnol 78:473–481