Heat-stable pullulanase from Bacillus acidopullulyticus: Characterization and refolding after guanidinium chloride-induced unfolding

Extremophiles

Volumn 3, Issue 2, 1999, Pages 147-152

  Stefanova, Miglena E ^a   Schwerdtfeger, Ruth ^b   Antranikian, Garabed ^b   Scandurra, Roberto ^c  

^a INSTITUTE OF MICROBIOLOGY   (Bulgaria)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Bacillus acidopullulyticus; Denaturation; Pullulanase; Renaturation; Thermostability

Indexed keywords

ENZYME ANALYSIS; ENZYME STRUCTURE; PULLULANASE; SPECTROSCOPY;

ANIMALS; BACILLUS; CATTLE; GLYCOSIDE HYDROLASES; GUANIDINE; HEATING; PROTEIN FOLDING;

BACILLUS ACIDOPULLULYTICUS; BACTERIA (MICROORGANISMS); BOVINAE;

EID: 0033136101     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920050110     Document Type: Article

References (36)

1. AnfinsenCB(1973)Principlesthatgovernthefoldingofproteinchains.Science181: 223-230
2. Bender H, Wallenfels K (1961) Untersuchungen an Pullulan. II. Spezifischer Abbau durch ein bakterielles. Enzyme Biochem Z 334:79-95
3. Bernfeld P (1955) Amylases a and b. Methods Enzymol 1:149-158
4. Creighton TE (1994) The protein folding problem. In: Pain RH (ed) Mechanisms of protein folding. IRL Press, Oxford, pp 1-25
5. Jaenicke R, Rudolph R (1989) Folding proteins, In: Creighton TE (ed) Protein structure - a practical approach. IRL Press, Oxford, pp 191-223
6. Knapp S, Rüdiger A, Antranikian G, Jorgensen PL, Ladenstein R (1995) Crystallization and preliminary cryslallographic analysis of an amylopullulanase from the hyperthermophilic archaeon Pyrococcus woesei. Proteins Struct Funct Genet 23:595-597
7. Koch R, Canganella F, Hippe H, Jahnke KD, Antranikian G (1997) Purification and properties of a thermostable pullulanase from a newly isolated thermophilic anaerobic bacterium, Fervidobacterium pennavorans Ven5. Appl Environ Microbiol 63:1088-1094
8. Kuriki T, Park J, Okada S, Imanaka T (1988) Purification and characterization of thermostable pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis. Appl Environ Microbiol 54:2881-2883
9. Kuriki T, Park J, Imanaka T (1990) Characteristics of thermostable pullulanase from Bacillus steamthermophilus and the nucleotide sequence of the gene. J Ferment Bioeng 69:204-210
10. Kusano S, Nagahata N, Takahashi S-I, Fujimoto D, Sakano Y (1988) Purification and properties of Bacillus acidopullnlyticus pullulanase. Agric Biol Chem 52:2293-2298
11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680-685
12. Lappalainen A, Niku-Paavola M-L, Suortti T, Poutanen K (1991) Purification and characterization of Bacillus acidopulluyticus pullulanase for enzymatic starch modification. Starch/Staerke 43: 477-482
13. Leatherbarrow RJ (1987) EnzFitter. A non-linear regression data analysis program for the IBM-PC. Elsevier-BIOSOFT. Cambridge, UK
14. Lilie H, Lang K, Rudolph R, Buchner J (1993) Prolyl isomerases catalyze antibody folding in vitro. Protein Sci 2:1490-1496
15. Maeda Y, Yamada H, Ueda T, Imoto T (1996) Effect of additives on the renaturation of reduced lysozyme in the presence of 4M urea. Protein Eng 9:461-465
16. Nakamura N, Watanabe K, Horikoshi K (1975) Purification and some properties of alkaline pullulanase from a strain of Bacillus no. 202-1, an alkalophilic microrganism. Biochim Biophys Acta 397:188-193
17. Norman BE (1983) A novel Bacillus pullulanase - its properties and application in the glucose syrups industry. J Jpn Soc Starch Sci 30:200-211
18. Odibo FJC, Obi SKC (1988) Purification and characterrization of thermostable pullulanase from Thermoactinomyces thalpophilus. J Ind Microbiol 3:343-350
19. Ohba R, Ueda S (1975) Some properties of crystalline extra- and intracellular pullulanase from Aerobacter aerogenes. Agric Biol Chem 39:967-972
20. Plant AR, Clemens RM, Daniel RM, Morgan HW (1987) Purification and preliminary characterization of an extracellular pullulanase from Thermoanaerobium Tok6-B1. Appl Microbiol Biotechnol 26:427-433
21. Rozema D, Gellman SH (1995) Artificial chaperones: protein refolding via sequential use of detergent and cyclodextrin. J Am Chem Soc 117:2373-2374
22. Rüdiger A, Jorgensen PL, Antranikian G (1995) Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli. Appl Environ Microbiol 61:567-575
23. Saha BC, Mathupala SP, Zeikus JG (1988) Purification and characterization of a highly thermostable novel pullulanase from Clostridium thermohydrosulfuricum. Biochem J 252:343-348
24. Schmid FX (1989) Spectral methods of characterizing protein conformation and conformational changes. In: Creighton TE (ed) Protein structure - a practical approach. IRL Press, Oxford, pp 251-285
25. Schulein M, Højer-Pedersen B (1984) Characterization of a new class of thermophilic pullulanases from Bacillus acidopullulyticus. Ann NY Acad Sci 434:271-274
26. Solovikova A, Gasperik J, Hostinova E (1996) High-yield production of Saccharomycopsis fibuligera glucoamylase in Escherichia coli, refolding, and comparison of the nonglycosylated and glycosylaled enzyme forms. Biochem Biophys Res Commun 224:790-795
27. Spreinat A, Antranikian G (1990) Purification and properties of a thermostable pullulanase from Clostridium thermosulfurogenes EM1 which hydrolyses both α-1.6- and α-1,4-glycosidic linkages. Appl Microbiol Biotechnol 33:511-518
28. Stigter D, Dill KA (1990) Charge effects on folded and unfolded proteins. Biochemistry 29:1262-1271
29. Suzuki Y, Hatagaki K, Oda H (1991) A hyperthermostable pullulanase produced by an extreme thermophile, Bacillus flavocaldarius KP 1228, and evidence for the proline theory of increasing protein thermostability. Appl Microbiol Biotechnol 34:707-714
30. Tams JW, Welinder KG (1996) Unfolding and refolding of Corpinus cinereus peroxidase at high pH, in urea, and at high temperature, Effect of organic and ionic additives on these processes. Biochemistry 35:7573-7579
31. Tandon S, Horowitz PM (1989) Reversible folding of rhodanese. Presence of intermediate(s) at equilibrium. J Biol Chem 264:9859-9866
32. Thatcher DR. Hitchcock A (1994) Protein folding in biotechnology. In: Pain RH (ed) Mechanisms of protein folding. IRL Press, Oxford, pp 229-261
33. Timasheff SN, Arakawa T (1989) Stabilization of protein structure by solvents. In: Creighton TE (ed) Protein structure - a practical approach. IRL Press, Oxford, pp 331-345
34. Vihinen M, Møntsølø P (1989) Microbial amylolytic enzymes. Crit Rev Biochem Mol Biol 24:329-418
35. Walker GJ (1968) Metabolism of the reserve polysaccharide of Streptococcus mitis. Some properties of a pullulanase. Biochem J 108:33-40
36. Zhi W, Landry SJ, Gierasch LM, Srere PA (1992) Renaturation of citrate synthase: influence of denaturant and folding assistants. Protein Sci 1:522-529