Positional effects of fusion partners on the yield and solubility of MBP fusion proteins

Protein Expression and Purification

Volumn 110, Issue , 2015, Pages 159-164

  Raran Kurussi, Sreejith ^a   Keefe, Karina ^a   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Fusion protein; Gateway cloning; Inclusion bodies; MBP; Solubility enhancer

Indexed keywords

ESCHERICHIA COLI;

ESCHERICHIA COLI PROTEIN; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; OLIGOPEPTIDE;

AMINO ACID SEQUENCE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; PLASMID; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; SOLUBILITY; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HISTIDINE; MALTOSE-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PLASMIDS; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84925411269     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2015.03.004     Document Type: Article

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