Engineering customized TALE nucleases (TALENS) and TALE transcription factors by fast ligation-based automatable solid-phase high-throughput (FLASH) assembly

Current Protocols in Molecular Biology

Volumn , Issue SUPPL.103, 2013, Pages

  Reyon, Deepak ^a,b   Maeder, Morgan L ^a,b   Khayter, Cyd ^a   Tsai, Shengdar Q ^a,b   Foley, Jonathan E ^a   Sander, Jeffry D ^a,b   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

FLASH; TAL effector; TALE; TALE activator; TALEN

Indexed keywords

DNA BINDING PROTEIN; DNA FRAGMENT; NUCLEASE; PLASMID VECTOR; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE; HYBRID PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA;

ARTICLE; BIOENGINEERING; COMPUTER PROGRAM; DOWN REGULATION; ENZYME ENGINEERING; FAST LIGATION BASED AUTOMATABLE SOLID PHASE HIGH THROUGHPUT ASSEMBLY; GENE CONSTRUCT; GENE EXPRESSION REGULATION; MOLECULAR CLONING; PIPETTE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; ROBOTICS; TANDEM REPEAT; AUTOMATION; DNA SEQUENCE; GENE TARGETING; GENE VECTOR; GENETIC ENGINEERING; GENETICS; GENOMICS; INTERNET; METABOLISM; PLASMID; PROCEDURES; SOLID PHASE SYNTHESIS; TIME; METHODOLOGY; AGAR GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; ANALYTIC METHOD; BIOTINYLATION; DNA FRAGMENTATION; FAST LIGATION BASED AUTOMATABLE SOLID PHASE HIGH THROUGHPUT ASSEMBLY METHOD; GENE EDITING; HUMAN; INCUBATION TIME; LIMIT OF QUANTITATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; ROOM TEMPERATURE; UPREGULATION;

AUTOMATION; CLONING, MOLECULAR; DEOXYRIBONUCLEASES; DNA LIGASES; DNA PRIMERS; GENE TARGETING; GENETIC ENGINEERING; GENETIC VECTORS; GENOMICS; INTERNET; PLASMIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SOFTWARE; SOLID-PHASE SYNTHESIS TECHNIQUES; TIME FACTORS; TRANSCRIPTION FACTORS;

EID: 84880815042     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb1216s103     Document Type: Article

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