Campenot cultures and microfl uidics provide complementary platforms for spatial study of dorsal root ganglia neurons

Neuromethods

Volumn 103, Issue , 2015, Pages 105-124

  Fenstermacher, Sara J ^a   Pazyra Murphy, Maria F ^a   Segal, Rosalind A ^a  

^a NONE

Author keywords

Axonal RNA; Campenot cultures; Compartmented culture; Dorsal root ganglia (DRG) neurons; Local protein synthesis; Microfluidics; Neuroscience; Neurotrophins

Indexed keywords

NEUROTROPHIN;

ADULT; ANIMAL CELL; ARTICLE; CAMPENOT CHAMBER; CELL CULTURE EQUIPMENT; CELL STIMULATION; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; EMBRYO; FEMALE; GENE EXPRESSION; MICROFLUIDICS; MOUSE; NERVE CELL CULTURE; NONHUMAN; PERIKARYON; PRIORITY JOURNAL; RAT; SENSORY NERVE CELL; SIGNAL TRANSDUCTION; SPINAL CORD NERVE CELL; SPINAL GANGLION;

RATTUS;

EID: 84924967230     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2510-0_6     Document Type: Article

References (35)

1. Segal RA (2003) Selectivity in neurotrophin signaling: theme and variations. Annu Rev Neurosci 26:299–330.
2. Bibel M, Barde YA (2000) Neurotrophins: key regulators of cell fate and cell shape in the vertebrate nervous system. Genes Dev 14(23):2919–2937.
3. Campenot RB (1977) Local control of neurite development by nerve growth factor. Proc Natl Acad Sci U S A 74(10):4516–4519.
4. Campenot RB (1982) Development of sympathetic neurons in compartmentalized cultures. II Local control of neurite growth by nerve growth factor. Dev Biol 93(1):1–12.
5. Campenot RB (1994) NGF and the local control of nerve terminal growth. J Neurobiol 25(6):599–611.
6. Campenot RB, Lund K, Mok SA (2009) Production of compartmented cultures of rat sympathetic neurons. Nat Protoc 4(12):1869–1887.
7. Karten B et al (2005) Neuronal models for studying lipid metabolism and transport. Methods 36(2):117–128.
8. Vance JE et al (1991) Biosynthesis of membrane lipids in rat axons. J Cell Biol 115(4):1061–1068.
9. Ginty DD, Segal RA (2002) Retrograde neurotrophin signaling: Trk-ing along the axon. Curr Opin Neurobiol 12(3):268–274.
10. Harrington AW, Ginty DD (2013) Longdistance retrograde neurotrophic factor signalling in neurons. Nat Rev Neurosci 14(3):177–187.
11. Pazyra-Murphy MF, Segal RA (2008) Preparation and maintenance of dorsal root ganglia neurons in compartmented cultures. J Vis Exp 20.
12. Watson FL et al (2001) Neurotrophins use the Erk5 pathway to mediate a retrograde survival response. Nat Neurosci 4(10):981–988.
13. Harrington AW et al (2011) Recruitment of actin modifi ers to TrkA endosomes governs retrograde NGF signaling and survival. Cell 146(3):421–434.
14. Kuruvilla R et al (2004) A neurotrophin signaling cascade coordinates sympathetic neuron development through differential control of TrkA traffi cking and retrograde signaling. Cell 118(2):243–255.
15. Riccio A et al (1997) An NGF-TrkA-mediated retrograde signal to transcription factor CREB in sympathetic neurons. Science 277(5329):1097–1100.
16. MacInnis BL, Senger DL, Campenot RB (2003) Spatial requirements for TrkA kinase activity in the support of neuronal survival and axon growth in rat sympathetic neurons. Neuropharmacology 45(7):995–1010.
17. Mok SA, Campenot RB (2007) A nerve growth factor-induced retrograde survival signal mediated by mechanisms downstream of TrkA. Neuropharmacology 52(2):270–278.
18. Mok SA et al (2013) A HaloTag(R) method for assessing the retrograde axonal transport of the p75 neurotrophin receptor and other proteins in compartmented cultures of rat sympathetic neurons. J Neurosci Methods 214(1):91–104.
19. Park JW et al (2006) Microfl uidic culture platform for neuroscience research. Nat Protoc 1(4):2128–2136.
20. Jung H, Yoon BC, Holt CE (2012) Axonal mRNA localization and local protein synthesis in nervous system assembly, maintenance and repair. Nat Rev Neurosci 13(5):308–324.
21. Andreassi C et al (2010) An NGF-responsive element targets myo-inositol monophosphatase- 1 mRNA to sympathetic neuron axons. Nat Neurosci 13(3):291–301.
22. Gumy LF et al (2011) Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization. RNA 17(1):85–98.
23. Taylor AM et al (2009) Axonal mRNA in uninjured and regenerating cortical mammalian axons. J Neurosci 29(15):4697–4707.
24. Willis D et al (2005) Differential transport and local translation of cytoskeletal, injuryresponse, and neurodegeneration protein mRNAs in axons. J Neurosci 25(4):778–791.
25. Dieterich DC et al (2006) Selective identifi cation of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc Natl Acad Sci U S A 103(25):9482–9487.
26. Eng H, Lund K, Campenot RB (1999) Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures. J Neurosci 19(1):1–9.
27. Kar AN et al (2013) Intra-axonal synthesis of eukaryotic translation initiation factors regulates local protein synthesis and axon growth in rat sympathetic neurons. J Neurosci 33(17):7165–7174.
28. Cosker KE et al (2013) Target-derived neurotrophins coordinate transcription and transport of bclw to prevent axonal degeneration. J Neurosci 33(12):5195–5207.
29. Willis DE et al (2007) Extracellular stimuli specifi cally regulate localized levels of individual neuronal mRNAs. J Cell Biol 178(6):965–980.
30. Chao JA, Yoon YJ, Singer RH (2012) Imaging translation in single cells using fl uorescent microscopy. Cold Spring Harb Perspect Biol 4(11).
31. Dieterich DC et al (2010) In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons. Nat Neurosci 13(7):897–905.
32. Tyagi S (2009) Imaging intracellular RNA distribution and dynamics in living cells. Nat Methods 6(5):331–338.
33. Wu B, Chao JA, Singer RH (2012) Fluorescence fl uctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. Biophys J 102(12):2936–2944.
34. Kimpinski K, Campenot RB, Mearow K (1997) Effects of the neurotrophins nerve growth factor, neurotrophin-3, and brainderived neurotrophic factor (BDNF) on neurite growth from adult sensory neurons in compartmented cultures. J Neurobiol 33(4):395–410.
35. Pacifi ci M, Peruzzi F (2012) Isolation and culture of rat embryonic neural cells: a quick protocol. J Vis Exp 63:e3965 Sara J. Fenstermacher et al.