Isolation and culture of rat embryonic neural cells: A quick protocol

Journal of Visualized Experiments

Volumn , Issue 63, 2012, Pages

  Pacifici, Marco ^a,b   Peruzzi, Francesca ^a,b  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Brain; Cell culture; Cortex; Isolation; Issue 63; Neuron; Neuroscience; Rat

Indexed keywords

EID: 84863745193     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3965     Document Type: Article

References (10)

1. Aprea, S., et al. Tubulin-mediated binding of human immunodeficiency virus-1 Tat to the cytoskeleton causes proteasomal-dependent degradation of microtubule-associated protein 2 and neuronal damage. J. Neurosci. 26, 4054-62 (2006).
2. Kivell, B.M., McDonald, F.J., & Miller, J.H. Serum-free culture of rat post-natal and fetal brainstem neurons. Brain Res. Dev. Brain Res. 120, 199-210 (2000).
3. Kivell, B.M., McDonald, F.J., & Miller, J.H. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons. Brain Res. Brain Res. Protoc. 6, 91-9 (2001).
4. Brewer, G.J. Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus. J. Neurosci. Res. 42, 674-83 (1995).
5. Banker, G., G.K. Culturing nerve cells, The MIT Press, Cambridge (1998).
6. Eletto, D., et al. Inhibition of SNAP25 expression by HIV-1 Tat involves the activity of mir-128a. J. Cell Physiol. 216, 764-70 (2008).
7. Gualco, E., et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ. 17 (3), 439-51 (2010).
8. Gage, F.H., et al. Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc. Natl. Acad. Sci. U.S.A. 92, 11879-83 (1995).
9. Keyser, D.O. & Pellmar, T.C. Synaptic transmission in the hippocampus: critical role for glial cells. Glia. 10, 237-43 (1994).
10. Pfrieger, F.W. & Barres, B.A. Synaptic efficacy enhanced by glial cells in vitro. Science. 277, 1684-7 (1997).