Rapid detection of viable Bacillus cereus emetic and enterotoxic strains in food by coupling propidium monoazide and multiplex PCR (PMA-mPCR)

Food Control

Volumn 55, Issue , 2015, Pages 151-157

  Forghani, Fereidoun ^a   Langaee, Taimour ^b   Eskandari, Mohammad ^c   Seo, Kun Ho ^d   Chung, Mi Ja ^e   Oh, Deog Hwan ^a  

^a Kangwon National University   (South Korea)

^b UNIVERSITY OF FLORIDA HEALTH   (United States)

^c ISLAMIC AZAD UNIVERSITY   (Iran)

^d Konkuk University   (South Korea)

^e Kwangju University   (South Korea)

Author keywords

Bacillus cereus; Emetic toxin; Enterotoxin; Food; Multiplex PCR; Propidium monoazide (PMA)

Indexed keywords

BACILLUS CEREUS;

EID: 84924931933     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2015.02.049     Document Type: Article

References (35)

1. Àlvarez G., González M., Isabal S., Blanc V., Berríos R.L. Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide. AMB Express 2013, 3(1):1-8.
2. Buchanan R.L., Schultz F.J. Comparison of Tecra VIA kit, Oxoid BCET-RPLA kit and CHO cell culture assay for the detection of Bacillus cereus diarrhoeal enterotoxin. Letters in Applied Microbiology 1994, 19:353-356.
3. Dierick K., Van Coillie E., Swiecicka I., Meyfroidt G., Devlieger H., Meulemans, et al. Fatal family outbreak of Bacillus cereus-associated food poisoning. Journal of Clinical Microbiology 2005, 43:4277-4279.
4. Drobniewski F.A. Bacillus cereus and related species. Clinical Microbiology Reviews 1993, 6:324-338.
5. Ehling-Schulz M., Fricker M., Siegfried S. Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay. FEMS Microbiology Letters 2004, 232:189-195.
6. Elizaquível P., Gabaldón J.A., Aznar R. Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control 2011, 22:158-164.
7. Elizaquível P., Sánchez G., Selma M.V., Aznar R. Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157:H7 in fresh-cut vegetable wash water. Food Microbiology 2012, 30:316-320.
8. Forghani F., Kim J.B., Oh D.H. Enterotoxigenic profiling of emetic toxin- and enterotoxin-producing Bacillus cereus, isolated from food, environmental, and clinical samples by multiplex PCR. Journal of Food Science 2014, 10.1111/1750-3841.12666.
9. Fricker M., Messelhäuer U., Scherer S., Ehling-Schulz M. Diagnostic real-time PCR assay for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Applied and Environmental Microbiology 2007, 73(6):1892-1898.
10. Fricker M., Reissbrodt R., Ehling-Schulz M. Evaluation of standard and new chromogenic selective plating media for isolation and identification of Bacillus cereus. International Journal of Food Microbiology 2008, 121(1):27-34.
11. Ghelardi E., Celandroni F., Salvetti S., Barsotti C., Baggiani A., Senesi S. Identification and characterization of toxigenic Bacillus cereus isolates responsible for two food-poisoning outbreaks. FEMS Microbiology Letters 2002, 208:129-134.
12. Hyeon J.Y., Park C., Choi I.S., Holt P.S., Seo K.H. Development of a multiplex real-time PCR with internal amplification control for simultaneous detection of Salmonella and cronobacter in powdered infant formula. International Journal of Food Microbiology 2010, 144:177-181.
13. Kim J.M., Forghani F., Kim J.B., Park Y.B., Park M.S., Wang J., et al. Improved multiplex PCR assay for simultaneous detection of Bacillus cereus emetic and enterotoxic strains. Food Science and Biotechnology 2012, 21(5):1439-1444.
14. Kim G.H., Forghani F., Oh D.H. Rapid detection of emetic toxin producing Bacillus cereus strains using triple-primer polymerase chain reaction (PCR) assay. African Journal of Microbiology Research 2013, 7(8):620-625.
15. Kim S.K., Kim K.P., Jang S.S., Shin E.M., Kim M.J., Oh S., et al. Prevalence and toxigenic profiles of Bacillus cereus isolated from dried red peppers, rice and sunsik in Korea. Journal of Food Protection 2009, 72:578-582.
16. Kim J.B., Kim J.M., Kim C.H., Seo K.S., Park Y.B., Choi N.J., et al. Emetic toxin producing Bacillus cereus Korean isolates contain genes encoding diarrheal-related enterotoxins. International Journal of Food Microbiology 2010, 144:182-186.
17. Kotiranta A., Lounatmaa K., Haapasalo M. Epidemiology and pathogenesis of Bacillus cereus infection. Microbial Infections 2000, 2:189-198.
18. Ladeuze S., Lents N., Delbrassinne L., Hu X., Kahillon J. Antifungal activity displayed by cereulide, the emetic toxin produced by Bacillus cereus. Applied and Environmental Microbiology 2011, 77(7):2555-2558.
19. Martínez-Blanch J.F., Sánchez G., Garay E., Aznar R. Development of a real-time PCR assay for detection and quantification of enterotoxigenic members of Bacillus cereus group in food samples. International Journal of Food Microbiology 2009, 135:15-21.
20. Martínez-Blanch J.F., Sánchez G., Garay E., Aznar R. Evaluation of real-time PCR assay for the detection and quantification of Bacillus cereus group spores in food. Journal of Food Protection 2010, 73(8):1480-1485.
21. Martínez-Blanch J.F., Sánchez G., Garay E., Aznar R. Detection and quantification of viable Bacillus cereus in food by RT-qPCR. European Food Research and Technology 2011, 232:951-955.
22. Nakano S., Maeshima H., Matsumura A., Ohno K., Ueda S., Kuwabara Y., et al. APCR assay based on a sequence-characterized amplified region marker for detection of emetic Bacillus cereus. Journal of Food Protection 2004, 67(8):1694-1701.
23. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiological Methods 2006, 67:310-320.
24. Nogva H.K., Dromtorp S.M., Nissen H., Rudi K. Ethidium monoazizde for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechnology 2003, 34:804-813.
25. Park Y.B., Kim J.B., Shin S.W., Kim J.C., Cho S.H., Lee B.K., et al. Prevalence, genetic diversity, and antibiotic susceptibility of Bacillus cereus strains isolated from rice and cereals collected in Korea. Journal of Food Protection 2009, 72(3):612-617.
26. Priha O., Hallamaa K., Saarela M., Raaska L. Detection of Bacillus cereus group bacteria from cardboard and paper with real-time PCR. Journal of Industrial Microbiology and Biotechnology 2004, 31:161-169.
27. Wan C., Yang Y., Xu H., Aguilar Z.P., Liu C., Lai W., et al. Development of a propidium monoazide treatment combined with loop-mediated isothermal amplification (PMA-LAMP) assay for rapid detection of viable Listeria monocytogenes. International Journal of Food Science and Technology 2012, 47:2460-2467.
28. Wang L., Li Y., Mustapha A. Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR. Journal of Applied Microbiology 2009, 107(5):1719-1728.
29. Wehrle E., Didier A., Moravek M., Dietrich R., Märtlbauer E. Detection of Bacillus cereus with enteropathogenic potential by multiplex real-time PCR. Molecular and Cellular Probes 2010, 24:124-130.
30. Wu L.C., Horng J.T., Huang H.Y., Lin F.M., Huang H.D., Tsai M.F. Primer design for multiplex PCR using a genetic algorithm. Soft Computing 2007, 11:855-863.
31. Yáñez M.A., Nocker A., Soria-Soria E., Múrtula R., Martínez L., Catalán V. Quantification of viable Legionella cells using propidium monoazide combined with quantitative PCR. Journal of Microbiological Methods 2011, 85(2):124-130.
32. Yang I.C., Shih D.Y., Huang T.P., Huang Y.P., Wang J.Y., Pan T.M. Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. Journal of Food Protection 2005, 68:2123-2130.
33. Yang I.C., Shih D.Y.C., Wang J.Y., Pan A.M. Development of rapid real-time PCR and most-probable-number real-time PCR assays to quantify enterotoxigenic strains of the species in the Bacillus cereus group. Journal of Food Protection 2007, 70(12):2774-2781.
34. Zhang Z., Wang L., Xu H., Aguilar Z.P., Liu C., Gan B., et al. Detection of non-emetic and emetic Bacillus cereus by propidium monoazide multiplex PCR (PMA-mPCR) with internal amplification control. Food Control 2014, 35:401-406.
35. Zhao X., Wang J., Forghani F., Park J.H., Park M.S., Seo K.H., et al. Rapid detection of viable Escherichia coli O157 by coupling propidium monoazide with loop-mediated isothermal amplification. Journal of Microbiology and Biotechnology 2013, 23(12):1708-1716.