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Volumn 1024, Issue , 2013, Pages 19-40

Methods of analysis of dendritic cell-derived exosome-shuttle microRNA and its horizontal propagation between dendritic cells

Author keywords

Dendritic cells; Exosomes; microRNA function; microRNA transfer; microRNAs

Indexed keywords

MICRORNA; CULTURE MEDIUM;

EID: 84924848675     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-453-1_3     Document Type: Article
Times cited : (28)

References (53)
  • 1
    • 78149409316 scopus 로고    scopus 로고
    • Export of microRNAs and microRNA-protective protein by mammalian cells
    • Wang K, Zhang S, Weber J et al (2010) Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic Acids Res 38:7248-7259
    • (2010) Nucleic Acids Res , vol.38 , pp. 7248-7259
    • Wang, K.1    Zhang, S.2    Weber, J.3
  • 2
    • 79953202200 scopus 로고    scopus 로고
    • Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma
    • Arroyo JD, Chevillet JR, Kroh EM et al (2011) Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci USA 108:5003-5008
    • (2011) Proc Natl Acad Sci USA , vol.108 , pp. 5003-5008
    • Arroyo, J.D.1    Chevillet, J.R.2    Kroh, E.M.3
  • 3
    • 34249302620 scopus 로고    scopus 로고
    • Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
    • Valadi H, Ekstrom K, Bossios A et al (2007) Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9:654-659
    • (2007) Nat Cell Biol , vol.9 , pp. 654-659
    • Valadi, H.1    Ekstrom, K.2    Bossios, A.3
  • 4
    • 45449093192 scopus 로고    scopus 로고
    • MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer
    • Taylor DD, Gercel-Taylor C (2008) MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol 110:13-21
    • (2008) Gynecol Oncol , vol.110 , pp. 13-21
    • Taylor, D.D.1    Gercel-Taylor, C.2
  • 5
    • 57849130395 scopus 로고    scopus 로고
    • Detection of microRNA expression in human peripheral blood microvesicles
    • Piper Hunter M, Ismail N, Zhang X et al (2008) Detection of microRNA expression in human peripheral blood microvesicles. PLoS One 3:e3694
    • (2008) PLoS One , vol.3
    • Piper Hunter, M.1    Ismail, N.2    Zhang, X.3
  • 6
    • 57049103401 scopus 로고    scopus 로고
    • Gliobastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
    • Skog J, Wurdinger T, van Rijn S et al (2008) Gliobastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10:1470-1476
    • (2008) Nat Cell Biol , vol.10 , pp. 1470-1476
    • Skog, J.1    Wurdinger, T.2    Van Rijn, S.3
  • 7
  • 8
    • 0036676445 scopus 로고    scopus 로고
    • Exosomes: Composition, biogenesis and function
    • Thery C, Zitvogel L, Amigorena S (2002) Exosomes: Composition, biogenesis and function. Nat Rev Immunol 2:569-579
    • (2002) Nat Rev Immunol , vol.2 , pp. 569-579
    • Thery, C.1    Zitvogel, L.2    Amigorena, S.3
  • 9
    • 0023645106 scopus 로고
    • Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes)
    • Johnstone RM, Adam M, Hammond JR et al (1987) Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J Biol Chem 262:9412-9420
    • (1987) J Biol Chem , vol.262 , pp. 9412-9420
    • Johnstone, R.M.1    Adam, M.2    Hammond, J.R.3
  • 10
    • 0029989258 scopus 로고    scopus 로고
    • B lymphocytes secrete antigen-presenting vesicles
    • Raposo G, Nijman HW, Stoorvogel W et al (1996) B lymphocytes secrete antigen-presenting vesicles. J Exp Med 183:1161-1172
    • (1996) J Exp Med , vol.183 , pp. 1161-1172
    • Raposo, G.1    Nijman, H.W.2    Stoorvogel, W.3
  • 11
    • 0040089526 scopus 로고    scopus 로고
    • Molecular characterization of dendritic cellderived exosomes. Selective accumulation of the heat shock protein hsc73
    • Thery C, Regnault A, Garin J et al (1999) Molecular characterization of dendritic cellderived exosomes. Selective accumulation of the heat shock protein hsc73. J Cell Biol 147:599-610
    • (1999) J Cell Biol , vol.147 , pp. 599-610
    • Thery, C.1    Regnault, A.2    Garin, J.3
  • 12
    • 0030687649 scopus 로고    scopus 로고
    • Accumulation of major histocompatibility complex class II molecules in mast cell secretory granules and their release upon degranulation
    • Raposo G, Tenza D, Mecheri S et al (1997) Accumulation of major histocompatibility complex class II molecules in mast cell secretory granules and their release upon degranulation. Mol Biol Cell 8:2631-2645
    • (1997) Mol Biol Cell , vol.8 , pp. 2631-2645
    • Raposo, G.1    Tenza, D.2    Mecheri, S.3
  • 13
    • 0034909926 scopus 로고    scopus 로고
    • Intestinal epithelial cells secrete exosome-like vesicles
    • van Niel G, Raposo G, Candalh C et al (2001) Intestinal epithelial cells secrete exosome-like vesicles. Gastroenterology 121:337-349
    • (2001) Gastroenterology , vol.121 , pp. 337-349
    • Van Niel, G.1    Raposo, G.2    Candalh, C.3
  • 14
    • 20244373139 scopus 로고    scopus 로고
    • Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming
    • Wolfers J, Lozier A, Raposo G et al (2001) Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nat Med 7:297-303
    • (2001) Nat Med , vol.7 , pp. 297-303
    • Wolfers, J.1    Lozier, A.2    Raposo, G.3
  • 15
    • 0033485648 scopus 로고    scopus 로고
    • Activated platelets release two types of membrane vesicles: Microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules
    • Heijnen HF, Schiel AE, Fijnheer R et al (1999) Activated platelets release two types of membrane vesicles: Microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules. Blood 94:3791-3799
    • (1999) Blood , vol.94 , pp. 3791-3799
    • Heijnen, H.F.1    Schiel, A.E.2    Fijnheer, R.3
  • 16
    • 22044446912 scopus 로고    scopus 로고
    • Exosome-like vesicles are present in human blood plasma
    • Caby MP, Lankar D, Vincendeau-Scherrer C et al (2005) Exosome-like vesicles are present in human blood plasma. Int Immunol 17:879-887
    • (2005) Int Immunol , vol.17 , pp. 879-887
    • Caby, M.P.1    Lankar, D.2    Vincendeau-Scherrer, C.3
  • 17
    • 4444226979 scopus 로고    scopus 로고
    • Identi fication and proteomic pro filing of exosomes in human urine
    • Pisitkum T, Shen RF, Knepper MA (2004) Identi fication and proteomic pro filing of exosomes in human urine. Proc Natl Acad Sci USA 101:13368-13373
    • (2004) Proc Natl Acad Sci USA , vol.101 , pp. 13368-13373
    • Pisitkum, T.1    Shen, R.F.2    Knepper, M.A.3
  • 18
    • 0141962590 scopus 로고    scopus 로고
    • Exosomes with major histocompatibility complex II and co-stimulatory molecules are present in human BAL fluid
    • Admyre C, Grunewald J, Thyberg S et al (2003) Exosomes with major histocompatibility complex II and co-stimulatory molecules are present in human BAL fluid. Eur Respir J 22:578-583
    • (2003) Eur Respir J , vol.22 , pp. 578-583
    • Admyre, C.1    Grunewald, J.2    Thyberg, S.3
  • 19
    • 0037183242 scopus 로고    scopus 로고
    • Malignant effusions and immunogenic tumour-derived exosomes
    • Andre F, Schartz NE, Movassagh M et al (2002) Malignant effusions and immunogenic tumour-derived exosomes. Lancet 360: 295-305
    • (2002) Lancet , vol.360 , pp. 295-305
    • Andre, F.1    Schartz, N.E.2    Movassagh, M.3
  • 20
    • 0035877018 scopus 로고    scopus 로고
    • Proteomic analysis of dendritic cell-derived exosomes: A secreted subcellular compartment distinct from apoptotic vesicles
    • Thery C, Boussac M, Veron P et al (2001) Proteomic analysis of dendritic cell-derived exosomes: A secreted subcellular compartment distinct from apoptotic vesicles. J Immunol 166:7309-7318
    • (2001) J Immunol , vol.166 , pp. 7309-7318
    • Thery, C.1    Boussac, M.2    Veron, P.3
  • 21
    • 0013495952 scopus 로고    scopus 로고
    • Proteomic and biochemical analyses of human B cell-derived exosomes
    • Wubbolts R, Leckie RS, Veenhuizen PTM et al (2003) Proteomic and biochemical analyses of human B cell-derived exosomes. J Biol Chem 278:10963-10972
    • (2003) J Biol Chem , vol.278 , pp. 10963-10972
    • Wubbolts, R.1    Leckie, R.S.2    Veenhuizen, P.T.M.3
  • 23
    • 8644286802 scopus 로고    scopus 로고
    • Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells
    • Morelli AE, Larregina AT, Shufesky WJ et al (2004) Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells. Blood 104:3257-3266
    • (2004) Blood , vol.104 , pp. 3257-3266
    • Morelli, A.E.1    Larregina, A.T.2    Shufesky, W.J.3
  • 24
    • 22044447897 scopus 로고    scopus 로고
    • ICAM-1 on exosomes from mature dendritic cells is critical for ef ficient naive T-cell priming
    • Segura E, Nicco C, Lombard B et al (2005) ICAM-1 on exosomes from mature dendritic cells is critical for ef ficient naive T-cell priming. Blood 106:216-223
    • (2005) Blood , vol.106 , pp. 216-223
    • Segura, E.1    Nicco, C.2    Lombard, B.3
  • 25
    • 49149111236 scopus 로고    scopus 로고
    • Exosomes as a short-range mechanism to spread alloantigen between dendritic cells during T-cell allorecognition
    • Montecalvo A, Shufesky WJ, Beer Stolz D et al (2008) Exosomes as a short-range mechanism to spread alloantigen between dendritic cells during T-cell allorecognition. J Immunol 180:3081-3090
    • (2008) J Immunol , vol.180 , pp. 3081-3090
    • Montecalvo, A.1    Shufesky, W.J.2    Beer Stolz, D.3
  • 26
    • 74049102373 scopus 로고    scopus 로고
    • Human villous trophoblasts express and secrete placenta-speci fic microRNAs into maternal circulation via exosomes
    • Luo SS, Ishibashi O, Ishikawa G et al (2009) Human villous trophoblasts express and secrete placenta-speci fic microRNAs into maternal circulation via exosomes. Biol Reprod 81:717-729
    • (2009) Biol Reprod , vol.81 , pp. 717-729
    • Luo, S.S.1    Ishibashi, O.2    Ishikawa, G.3
  • 27
    • 73649123120 scopus 로고    scopus 로고
    • Exosomes from human saliva as a source of microRNA biomarkers
    • Michael A, Bajracharya SD, Yuen PST et al (2010) Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 16:34-38
    • (2010) Oral Dis , vol.16 , pp. 34-38
    • Michael, A.1    Bajracharya, S.D.2    Yuen, P.S.T.3
  • 28
    • 62549125455 scopus 로고    scopus 로고
    • Exosomal microRNA: A diagnostic marker for lung cancer
    • Rabinowitz G, Gercel-Taylor C, Day JM et al (2009) Exosomal microRNA: A diagnostic marker for lung cancer. Clin Lung Cancer 10:42-46
    • (2009) Clin Lung Cancer , vol.10 , pp. 42-46
    • Rabinowitz, G.1    Gercel-Taylor, C.2    Day, J.M.3
  • 29
    • 78149420582 scopus 로고    scopus 로고
    • Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line
    • Ohshima K, Inoue K, Fujiwara A et al (2010) Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line. PloS One 5:e13247
    • (2010) PloS One , vol.5
    • Ohshima, K.1    Inoue, K.2    Fujiwara, A.3
  • 30
    • 69949117622 scopus 로고    scopus 로고
    • Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity
    • Gibbings DJ, Ciaudo C, Erhardt M et al (2009) Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity. Nat Cell Biol 11:1143-1149
    • (2009) Nat Cell Biol , vol.11 , pp. 1143-1149
    • Gibbings, D.J.1    Ciaudo, C.2    Erhardt, M.3
  • 31
    • 0022201056 scopus 로고
    • Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes
    • Pan BT, Teng K, Wu C et al (1985) Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes. J Cell Biol 101:942-948
    • (1985) J Cell Biol , vol.101 , pp. 942-948
    • Pan, B.T.1    Teng, K.2    Wu, C.3
  • 32
    • 0021719707 scopus 로고
    • Endocytosis and intracellular processing of transferrin and colloidal-gold transferrin in rat reticulocytes: Demonstration of a pathway of receptor shedding
    • Harding C, Heuser J, Stahl P (1984) Endocytosis and intracellular processing of transferrin and colloidal-gold transferrin in rat reticulocytes: Demonstration of a pathway of receptor shedding. Eur J Cell Biol 35:256-263
    • (1984) Eur J Cell Biol , vol.35 , pp. 256-263
    • Harding, C.1    Heuser, J.2    Stahl, P.3
  • 33
    • 0034254524 scopus 로고    scopus 로고
    • Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface
    • Denzer K, van Eijk M, Kleijmeer MJ et al (2000) Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface. J Immunol 165:1259-1265
    • (2000) J Immunol , vol.165 , pp. 1259-1265
    • Denzer, K.1    Van Eijk, M.2    Kleijmeer, M.J.3
  • 34
    • 68849129712 scopus 로고    scopus 로고
    • Membrane vesicles as conveyors of immune responses
    • Thery C, Ostrowski M, Segura E (2009) Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 9:581-593
    • (2009) Nat Rev Immunol , vol.9 , pp. 581-593
    • Thery, C.1    Ostrowski, M.2    Segura, E.3
  • 35
    • 0031863853 scopus 로고    scopus 로고
    • Eradication of established murine tumors using a novel cell-free vaccine: Dendritic cell-derived exosomes
    • Zitvogel L, Regnault A, Lozier A et al (1998) Eradication of established murine tumors using a novel cell-free vaccine: Dendritic cell-derived exosomes. Nat Med 4:594-600
    • (1998) Nat Med , vol.4 , pp. 594-600
    • Zitvogel, L.1    Regnault, A.2    Lozier, A.3
  • 36
    • 84861164222 scopus 로고    scopus 로고
    • Induction of protective immunity against Eimeria tenella eimeira maxima, and eimeria acervulina infections using dc-derived exosomes
    • del Cacho E, Gallego M, Lee SH et al (2012) Induction of protective immunity against Eimeria tenella, Eimeira maxima, and Eimeria acervulina infections using DC-derived exosomes. Infect Immun 80(5):1909-1916
    • (2012) Infect Immun , vol.80 , Issue.5 , pp. 1909-1916
    • Del Cacho, E.1    Gallego, M.2    Lee, S.H.3
  • 37
    • 20844458756 scopus 로고    scopus 로고
    • Exosomes derived from IL-10-treated dendritic cells can suppress in flammation and collagen induced arthritis
    • Kim SH, Lechman ER, Bianco N et al (2005) Exosomes derived from IL-10-treated dendritic cells can suppress in flammation and collagen induced arthritis. J Immunol 174:6440-6448
    • (2005) J Immunol , vol.174 , pp. 6440-6448
    • Kim, S.H.1    Lechman, E.R.2    Bianco, N.3
  • 38
    • 33745424807 scopus 로고    scopus 로고
    • Induction of tolerance by exosomes and shortterm immunosuppression in a fully MHCmismatched rat cardiac allograft model
    • Peche H, Renaudin K, Beriou G et al (2005) Induction of tolerance by exosomes and shortterm immunosuppression in a fully MHCmismatched rat cardiac allograft model. Am J Transplant 6:1541-1550
    • (2005) Am J Transplant , vol.6 , pp. 1541-1550
    • Peche, H.1    Renaudin, K.2    Beriou, G.3
  • 39
    • 33644891591 scopus 로고    scopus 로고
    • The immune regulatory effect of apoptotic cells and exosomes on dendritic cells: Its impact on transplantation
    • Morelli AE (2006) The immune regulatory effect of apoptotic cells and exosomes on dendritic cells: Its impact on transplantation. Am J Transplant 6:254-261
    • (2006) Am J Transplant , vol.6 , pp. 254-261
    • Morelli, A.E.1
  • 40
    • 49049116385 scopus 로고    scopus 로고
    • Exosomes from bronchoalveolar fluid of tolerized mice prevent allergic reaction
    • Prado N, Marazuela EV, Segura E et al (2008) Exosomes from bronchoalveolar fluid of tolerized mice prevent allergic reaction. J Immunol 181:1519-1525
    • (2008) J Immunol , vol.181 , pp. 1519-1525
    • Prado, N.1    Marazuela, E.V.2    Segura, E.3
  • 42
    • 3042788972 scopus 로고    scopus 로고
    • Cells release prions in association with exosomes
    • Fevrier B, Vilette D, Archer F et al (2004) Cells release prions in association with exosomes. Proc Natl Acad Sci USA 101: 9683-9688
    • (2004) Proc Natl Acad Sci USA , vol.101 , pp. 9683-9688
    • Fevrier, B.1    Vilette, D.2    Archer, F.3
  • 43
    • 33746593662 scopus 로고    scopus 로고
    • Alzheimer's disease ® amyloid peptides are released in association with exosomes
    • Rajendran L, Honsho M, Zahn TR et al (2006) Alzheimer's disease ® -amyloid peptides are released in association with exosomes. Proc Natl Acad Sci USA 103:11172-11177
    • (2006) Proc Natl Acad Sci USA , vol.103 , pp. 11172-11177
    • Rajendran, L.1    Honsho, M.2    Zahn, T.R.3
  • 45
    • 79955070767 scopus 로고    scopus 로고
    • Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells
    • doi: 10:1038/ncomms1285
    • Mittelbrunn M, Gutierrez-Vasquez C, Villarroya-Beltri C et al (2011) Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Nat Commun 2:282. doi: 10:1038/ncomms1285
    • (2011) Nat Commun , vol.2 , pp. 282
    • Mittelbrunn, M.1    Gutierrez-Vasquez, C.2    Villarroya-Beltri, C.3
  • 46
    • 84862908547 scopus 로고    scopus 로고
    • Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes
    • Montecalvo A, Larregina AT, Shufesky WJ et al (2012) Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes. Blood 119:756-766
    • (2012) Blood , vol.119 , pp. 756-766
    • Montecalvo, A.1    Larregina, A.T.2    Shufesky, W.J.3
  • 47
    • 79953858598 scopus 로고    scopus 로고
    • Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
    • Alvarez-Erviti L, Seow Y, Yin HF et al (2011) Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol 19:341-345
    • (2011) Nat Biotechnol , vol.19 , pp. 341-345
    • Alvarez-Erviti, L.1    Seow, Y.2    Yin, H.F.3
  • 48
    • 0035469894 scopus 로고    scopus 로고
    • Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation
    • Morelli AE, Zahorchak AF, Larregina AT et al (2001) Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation. Blood 98:1512-1523
    • (2001) Blood , vol.98 , pp. 1512-1523
    • Morelli, A.E.1    Zahorchak, A.F.2    Larregina, A.T.3
  • 49
    • 77957952606 scopus 로고    scopus 로고
    • Endogenous dendritic cells mediate the effects of intravenously injected therapeutic immunosuppressive dendritic cells in transplantation
    • Divito SJ, Montecalvo A, Wang Z et al (2010) Endogenous dendritic cells mediate the effects of intravenously injected therapeutic immunosuppressive dendritic cells in transplantation. Blood 116:2694-2705
    • (2010) Blood , vol.116 , pp. 2694-2705
    • Divito, S.J.1    Montecalvo, A.2    Wang, Z.3
  • 50
    • 34547152610 scopus 로고    scopus 로고
    • Tolerogenic dendritic cells and the quest for transplant tolerance
    • Morelli AE, Thomson AW (2007) Tolerogenic dendritic cells and the quest for transplant tolerance. Nat Rev Immunol 7:610-621
    • (2007) Nat Rev Immunol , vol.7 , pp. 610-621
    • Morelli, A.E.1    Thomson, A.W.2
  • 51
    • 0037114287 scopus 로고    scopus 로고
    • Production and characterization of clinical grade exosomes derived from dendritic cells
    • Lamparski HG, Metha-Damani A, Yao J-Y et al (2002) Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Methods 270: 211-226
    • (2002) J Immunol Methods , vol.270 , pp. 211-226
    • Lamparski, H.G.1    Metha-Damani, A.2    Yao, J.-Y.3
  • 52
    • 77953291167 scopus 로고    scopus 로고
    • Critical evaluation of nanoparticle tracking analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates
    • Filipe V, Hawe A, Jiskoot W (2010) Critical evaluation of nanoparticle tracking analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates. Pharm Res 27:796-810
    • (2010) Pharm Res , vol.27 , pp. 796-810
    • Filipe, V.1    Hawe, A.2    Jiskoot, W.3
  • 53
    • 42749085108 scopus 로고    scopus 로고
    • Isolation and characterization of exosomes from cell culture supernatants and biological fluids
    • Thery C, Clayton A, Amigorena S et al (2006) Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protocol Cell Biol 3:3.22.1- 3.22.29
    • (2006) Curr Protocol Cell Biol , vol.3 , Issue.3 , pp. 2213-2229
    • Thery, C.1    Clayton, A.2    Amigorena, S.3


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