Production and characterization of clinical grade exosomes derived from dendritic cells

Journal of Immunological Methods

Volumn 270, Issue 2, 2002, Pages 211-226

  Lamparski, Henry G ^a   Metha Damani, Anita ^a   Yao, Jenq Yuan ^a   Patel, Sanjay ^a   Hsu, Di Hwei ^a   Ruegg, Curtis ^a   Le Pecq, Jean Bernard ^a  

^a Anosys Inc   (United States)

Author keywords

Characterization; Clinical grade exosomes; Manufacturing; Purification

Indexed keywords

DEUTERIUM OXIDE; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; SUCROSE; VACCINE;

ANTIGEN PRESENTING CELL; ARTICLE; CELL DENSITY; CELL SIZE; CELL VACUOLE; CONTROLLED STUDY; DENDRITIC CELL; EXOSOME; HUMAN; HUMAN CELL; MAJOR HISTOCOMPATIBILITY COMPLEX; MEMBRANE; MOLECULAR WEIGHT; MONOCYTE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PURIFICATION; QUALITY CONTROL; STANDARDIZATION; SUPERNATANT; ULTRACENTRIFUGATION; ULTRAFILTRATION;

ALBUMINS; ANTIGENS, CD; CENTRIFUGATION; CULTURE MEDIA; CYTOPLASMIC VESICLES; DENDRITIC CELLS; HAPTOGLOBINS; HISTOCOMPATIBILITY ANTIGENS CLASS II; HUMANS; IMMUNOPHENOTYPING; KINETICS;

EID: 0037114287     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00330-7     Document Type: Article

References (34)

1. Andre, F., Andersen, M., Wolfers, J., Lozier, A., Raposo, G., Serra, V., Ruegg, C., Fament, C., Angevin, E., Amigorena, S., Zitvogel, L., 2001. Exosomes in cancer immunotherapy: preclinical data. In: Mackiewicz, A. (Ed.), Progress in Basic and Clinical Immunology. Kluwer Academic/Plenum Publishers, New York, pp. 349-354.
2. Arnold P.Y., Mannie M.D. Vesicles bearing MHC class II molecules mediate transfer of antigen-presenting cells to CD4+ cells. Eur. J. Immunol. 29:1999;1363-1373.
3. Bhardwaj N., Friedman S.M., Cole B.C., Nisanian A.J. Dendritic cells are potent antigen-presenting cells for microbial superantigen. J. Exp. Med. 175:1992;267-273.
4. Bhardwaj N., Young J.W., Nisanian A.J., Baggers J., Steinman R.M. Small amounts of superantigen, when presented on dendritic cells, are sufficient to initiate T cell responses. J. Exp. Med. 178:1993;633-642.
5. Cella M., Engering A., Pinet V., Pieters J., Lanzavecchia A. Inflammatory stimuli induce accumulation of MHC class II complexes on dendritic cells. Nature. 388:1997;782-787.
6. Clayton A., Court J., Navabi H., Adams M., Mason M., Hobot J.A., Newman G.R., Jasani B. Analysis of antigen presenting cell-derived exosomes, through immuno-magnetic isolation and flow cytometry. J. Immunol. Methods. 247:2001;163-174.
7. Craunic R., Wu R., Otelea D., Georgescu M.M., Delperoux F., Guillot S., Balanant J., Tardy-Panit M. The replacement of water with deuterium oxide significantly improves the thermal stability of the oral poliovirus vaccine. Dev. Biol. Stand. 87:1996;161-166.
8. Denzer K., Eijk M.V., Kleijmeer M.J., Jakobson E., Groot C.D., Gueze H.J. Follicular dendritic cells carry MHC II-expressing microvesicles at their surface. J. Immunol. 165:2000;1259-1265.
9. Denzer K., Kleijmeer M.J., Heijnen H.F.G., Stoorvogel W., Geuze H.J. Exosomes: from internal vesicle of the multivesicular body to intercellular signaling device. J. Cell Sci. 113:2000;3365-3374.
10. Dobryyszycka W. Biological functions of haptoglobin-new pieces to an old puzzle. Eur. J. Clin. Chem. Clin. Biochem. 35:1997;647-654.
11. Escola J.-M., Kleijmeer M.J., Stoorvogel W., Griffith J.M., Yoshie O., Gueze H.J. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J. Biol. Chem. 273:1998;20121-20127.
12. Geminard C., Nault F., Johnstone R.M., Vidal M. Characteristics of the interaction between Hsc70 and the transferrin receptor in exosomes released during reticulocyte maturation. J. Biol. Chem. 276:2001;9910-9916.
13. Heijnen H.F.G., Schiel A.E., Fijnheer R., Gueze H.J., Sixma J.J. Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules. Blood. 94:1999;3791-3799.
14. Hess C., Sadallah S., Hefti A., Landmann R., Schifferli J.-A. Ectosomes released by human neutrophils are specialized functional units. J. Immunol. 163:1999;4564-4573.
15. Jensen L.B., Dam J., Teisner B. Identification and removal of polymer- and aggregate-forming proteins in human plasma albumin preparations. Vox Sang. 67:1994;125-131.
16. Johnstone R.M., Adam M., Hammond J.R., Orr L., Turbide C. Vesicle formation during reticulocyte maturation. J. Biol. Chem. 262:1987;9412-9420.
17. Jones P.J.H., Leatherdale S.T. Stable isotopes in clinical research: safety reaffirmed. Clin. Sci. 80:1991;277-280.
18. Kovacsovics-Bankowski M., Clark K., Benacerraf B., Rock K. Efficient major histocompatibility complex class I presentation of exogenous antigen upon phagocytosis by macrophage. Proc. Natl. Acad. Sci. U. S. A. 90:1993;4942-4946.
19. Kushner D.J., Baker A., Dunstall T.G. Pharmacological uses and perspectives of heavy water and denatured compounds. Can. J. Physiol. Pharm. 77:1999;79-88.
20. Milstien J.B., Lemon S. Regulatory considerations for new stabilized polio vaccines. Dev. Biol. Stand. 87:1996;181-189.
21. Morse M.A., Garst J., Khan S., Compagna L., Hobeika A., Colling K., Ghanayem M., Heck R., Valente N., Pecq J.B.L., Clay T.M. Preliminary results of a phase I/II study of active immunotherapy with autologous dexosomes loaded with MAGE peptides in HLA A2+ patients with stage III/IV non-small cell lung cancer. Proc. Am. Soc. Oncol. 21(A42):2002;11a.
22. Oh S.-K., Kim S.-H., Walker J.E. Interference with immune response at the level of generating effector cells by tumor-associated haptoglobin. J. Natl. Cancer Inst. 82:1990;11.
23. Patel D.M., Arnold P.Y., White G.A., Nardella J.P., Mannie M.D. Class II MHC/peptide complexes are released from APC and are acquired by T cell responders during specific antigen recognition. J. Immunol. 163:1999;5201.
24. Quah B., O'Niel H.C. The application of dendritic cell-derived exosomes in tumour immunotherapy. Cancer Biother. Radiopharm. 15:2000;185-194.
25. Raposa G., Tenza D., Mecheri S., Peronet R., Bonnerot C., Desaymond C. Accumulation of major histocompatibility complex class II molecules in mast cell secretory granules and their release upon degranulation. Mol. Biol. Cell. 8:1997;2631-2645.
26. Raposo G., Nijman H.W., Stoorvogel W., Leijendekker R., Harding C.V., Melief C.J., Gueze H.J. B lymphocytes secrete antigen-presenting vesicles. J. Exp. Med. 183:1996;1161-1172.
27. Skokos D., Panse S.L., Villa I., Rousselle J.-C., Peronet R., David B., Namane A., Mercheri S. Mast cell-dependent B and T-lymphocyte activation is mediated by the secretion of immunologically active exosomes. J. Immunol. 166:2001;868-876.
28. Thery C., Regnault A., Garin J., Wolfers J., Zitvogel L., Ricciardi-Castagnoli P., Raposo G., Amigorena S. Molecular characterization of dendritic cell-derived exosomes: selective accumulation of the heat shock protein Hsc73. J. Cell Biol. 147:1999;599-610.
29. Thery C., Boussac M., Veron P., Ricciardi-Castagnoli P., Raposo G., Garin J., Amigorena S. Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. J. Immunol. 166:2001;7309-7318.
30. Thery C., Zitvogel L., Amigorena S. Exosomes: composition, biogenesis, and function. Nat. Immunol. 2:2002;1-11.
31. Wolfers J., Lozier A., Raposo G., Regnault A., Thery C., Masurier C., Flament C., Pouzieux S., Faure F., Tursz T., Angevin E., Amigorena S., Zitvogel L. Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nat. Med. 7:2001;297-303.
32. 2O). Vaccine. 13:1995;1058-1063.
33. Yee E., Brown J.E. Quantitation of human haptoglobin: comparative ELISA studies using adsorption and capture methods. J. Immunol. Methods. 225:1999;125-130.
34. Zitvogel L., Regnault A., Lozier A., Wolfers J., Flament C., Tenza D., Ricciardi-Castagnoli P., Raposo G., Amigorena S. Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes. Nat. Med. 4:1998;594-600.