Oleuropein induces apoptosis via activation of caspases and suppression of phosphatidylinositol 3-kinase/protein kinase B pathway in HepG2 human hepatoma cell line

Molecular Medicine Reports

Volumn 11, Issue 6, 2015, Pages 4617-4624

  Yan, Chun Mei ^a   Chai, Er Qing ^a   Cai, Hong Yi ^a   Miao, Guo Ying ^a   Ma, Wen ^a  

^a GANSU PROVINCIAL HOSPITAL   (China)

Author keywords

Apoptosis; Hepatocellular carcinoma; Oleuropein; Proliferation; Protein kinase B

Indexed keywords

CYTOKINE RECEPTOR; JANUS KINASE; LIPOPOLYSACCHARIDE; ANTINEOPLASTIC AGENT; CASPASE; GLUTATHIONE; IRIDOID; OLEUROPEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN BAX; PROTEIN KINASE B; REACTIVE OXYGEN METABOLITE;

ACUTE LUNG INJURY; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ARTIFICIAL VENTILATION; BIOLOGICAL FUNCTIONS; CATALYSIS; CLUSTER ANALYSIS; CONTROLLED STUDY; CYTOKINE RESPONSE; DNA MICROARRAY; GENE EXPRESSION; GENE INTERACTION; HISTOPATHOLOGY; IMMUNE RESPONSE; INFLAMMATION; LIPOPOLYSACCHARIDE INDUCED ACUTE LUNG INJURY; MALE; MOUSE; NONHUMAN; PHARMACEUTICAL CARE; RANDOMIZED CONTROLLED TRIAL; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; VENTILATOR INDUCED LUNG INJURY; APOPTOSIS; CHEMISTRY; DRUG EFFECTS; GENETICS; HEPG2 CELL LINE; HUMAN; LIVER CELL CARCINOMA; LIVER TUMOR; METABOLISM; PATHOLOGY; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CARCINOMA, HEPATOCELLULAR; CASPASES; CELL LINE, TUMOR; GLUTATHIONE; HEP G2 CELLS; HUMANS; IRIDOIDS; LIVER NEOPLASMS; PHOSPHATIDYLINOSITOL 3-KINASE; PROTO-ONCOGENE PROTEINS C-AKT; REACTIVE OXYGEN SPECIES; SIGNAL TRANSDUCTION;

EID: 84924692295     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2015.3266     Document Type: Article

References (42)

1. Llovet JM, Burroughs A and Bruix J: Hepatocellular carcinoma. Lancet 362: 1907-1917, 2003.
2. Singh S, Singh PP, Roberts LR and Sanchez W: Chemopreventive strategies in hepatocellular carcinoma. Nat Rev Gastroenterol Hepatol 11: 45-54, 2013.
3. Cheng JW and Lv Y: New progress of non-surgical treatments for hepatocellular carcinoma. Med Oncol 30: 381, 2013.
4. Bruix J and Sherman M; American Association for the Study of Liver Diseases: Management of hepatocellular carcinoma: an update. Hepatology 53: 1020-1022, 2011.
5. Bravi F, Bosetti C, Tavani A, Gallus S and La Vecchia C: Coffee reduces risk for hepatocellular carcinoma: an updated meta-analysis. Clin Gastroenterol Hepatol 11: 1143-1421, 2013.
6. Calvisi DF, Ladu S, Hironaka K, Factor VM and Thorgeirsson SS: Vitamin E down-modulates iNOS and NADPH oxidase in c-Myc/TGF-alpha transgenic mouse model of liver cancer. J Hepatol 41: 815-822, 2004.
7. Sawada N, Inoue M, Iwasaki M, et al: Consumption of n-3 fatty acids and fish reduces risk of hepatocellular carcinoma. Gastroenterology 142: 1468-1475, 2012.
8. Hursting SD, Thornquist M and Henderson MM: Types of dietary fat and the incidence of cancer at five sites. Prev Med 19: 242-253, 1990.
9. Liu K, Stamler J, Moss D, Garside D, Persky V and Soltero I: Dietary cholesterol, fat, and fibre, and colon-cancer mortality. An analysis of international data. Lancet 2: 782-785, 1979.
10. Ferro-Luzzi A and Sette S: The Mediterranean Diet: an attempt to define its present and past composition. Eur J Clin Nutr 43 Suppl 2: 13-29, 1989.
11. Andreadou I, Mikros E, Ioannidis K, et al: Oleuropein prevents doxorubicin-induced cardiomyopathy interfering with signaling molecules and cardiomyocyte metabolism. J Mol Cell Cardiol 69: 4-16, 2014.
12. Hassan ZK, Elamin MH, Omer SA, et al: Oleuropein induces apoptosis via the p53 pathway in breast cancer cells. Asian Pac J Cancer Prev 14: 6739-6742, 2013.
13. Bulotta S, Corradino R, Celano M, et al: Antioxidant and antigrowth action of peracetylated oleuropein in thyroid cancer cells. J Mol Endocrinol 51: 181-189, 2013.
14. Cardeno A, Sánchez-Hidalgo M, Rosillo MA and Alarcón de la Lastra C: Oleuropein, a secoiridoid derived from olive tree, inhibits the proliferation of human colorectal cancer cell through downregulation of HIF-1-. Nutr Cancer 65: 147-156, 2013.
15. Cotter TG: Apoptosis and cancer: the genesis of a research field. Nat Rev Cancer 9: 501-507, 2009.
16. Johnstone RW, Ruefli AA and Lowe SW: Apoptosis: a link between cancer genetics and chemotherapy. Cell 108: 153-164, 2002.
17. Kumar A, Rajendran V, Sethumadhavan R and Purohit R: AKT kinase pathway: a leading target in cancer research. Scientific World Journal 2013: 756134, 2013.
18. Pavlidou A and Vlahos NF: Molecular alterations of PI3K/Akt/mTOR pathway: a therapeutic target in endometrial cancer. Scientific World Journal 2014: 709736, 2014.
19. Franke TF, Hornik CP, Segev L, Shostak GA and Sugimoto C: PI3K/Akt and apoptosis: size matters. Oncogene 22: 8983-8998, 2003.
20. Datta SR, Dudek H, Tao X, et al: Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91: 231-241, 1997.
21. Hahn-Windgassen A, Nogueira V, Chen CC, Skeen JE, Sonenberg N and Hay N: Akt activates the mammalian target of rapamycin by regulating cellular ATP level and AMPK activity. J Biol Chem 280: 32081-32089, 2005.
22. Rokutanda S, Fujita T, Kanatani N, et al: Akt regulates skeletal development through GSK3, mTOR, and FoxOs. Dev Biol 328: 78-93, 2009.
23. Adams JM and Cory S: Life-or-death decisions by the Bcl-2 protein family. Trends Biochem Sci 26: 61-66, 2001.
24. Nicholson KM and Anderson NG: The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14: 381-395, 2002.
25. Vlahos CJ, Matter WF, Hui KY and Brown RF: A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J Biol Chem 269: 5241-5248, 1994.
26. Nogueira V, Park Y, Chen CC, et al: Akt determines replicative senescence and oxidative or oncogenic premature senescence and sensitizes cells to oxidative apoptosis. Cancer Cell 14: 458-470, 2008.
27. Robey RB and Hay N: Is Akt the 'Warburg kinase' Akt-energy metabolism interactions and oncogenesis. Semin Cancer Biol 19: 25-31, 2009.
28. Fini L, Hotchkiss E, Fogliano V, et al: Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines. Carcinogenesis 29: 139-146, 2008.
29. La Vecchia C: Association between Mediterranean dietary patterns and cancer risk. Nutr Rev 67 Suppl 1: S126-S129, 2009.
30. Menendez JA, Papadimitropoulou A, Vellon L and Lupu R: A genomic explanation connecting 'Mediterranean diet', olive oil and cancer: oleic acid, the main monounsaturated fatty acid of olive oil, induces formation of inhibitory 'PEA3 transcription factor-PEA3 DNA binding site' complexes at the Her-2/neu (erbB-2) oncogene promoter in breast, ovarian and stomach cancer cells. Eur J Cancer 42: 2425-2432, 2006.
31. Kaufmann SH and Earnshaw WC: Induction of apoptosis by cancer chemotherapy. Exp Cell Res 256: 42-49, 2000.
32. Wiita AP, Ziv E, Wiita PJ, et al: Global cellular response to chemotherapy-induced apoptosis. Elife 29: e01236, 2013.
33. Czerski L and Nunez G: Apoptosome formation and caspase activation: is it different in the heart? J Mol Cell Cardiol 37: 643-652, 2004.
34. Fulda S and Debatin KM: Apoptosis signaling in tumor therapy. Ann NY Acad Sci 1028: 150-156, 2004.
35. Zhang L, Yu J, Park BH, Kinzler KW and Vogelstein B: Role of BAX in the apoptotic response to anticancer agents. Science 290: 989-992, 2000.
36. Fresno Vara JA, Casado E, de Castro J, Cejas P, Belda-Iniesta C and González-Barón M: PI3K/Akt signalling pathway and cancer. Cancer Treat Rev 30: 193-204, 2004.
37. Hennessy BT, Smith DL, Ram PT, Lu Y and Mills GB: Exploiting the PI3K/AKT pathway for cancer drug discovery. Nat Rev Drug Discov 4: 988-1004, 2005.
38. Minguez B, Tovar V, Chiang D, Villanueva A and Llovet JM: Pathogenesis of hepatocellular carcinoma and molecular therapies. Curr Opin Gastroenterol 25: 186-194, 2009.
39. Saxena NK, Sharma D, Ding X, et al: Concomitant activation of the JAK/STAT, PI3K/AKT, and ERK signaling is involved in leptin-mediated promotion of invasion and migration of hepatocellular carcinoma cells. Cancer Res 67: 2497-2507, 2007.
40. Wang GL, Iakova P, Wilde M, Awad S and Timchenko NA: Liver tumors escape negative control of proliferation via PI3K/Akt-mediated block of C/EBP alpha growth inhibitory activity. Genes Dev 18: 912-925, 2004.
41. Nogueira V, Park Y, Chen CC, et al: Akt determines replicative senescence and oxidative or oncogenic premature senescence and sensitizes cells to oxidative apoptosis. Cancer Cell 14: 458-470, 2008.
42. Liu J, Zhou J and Xing D: Phosphatidylinositol 3-kinase plays a vital role in regulation of rice seed vigor via altering NADPH oxidase activity. PLoS One 7: e33817, 2012.