RNA sequencing of mouse sinoatrial node reveals an upstream regulatory role for Islet-1 in cardiac pacemaker cells

Circulation Research

Volumn 116, Issue 5, 2015, Pages 797-803

  Vedantham, Vasanth ^a,b,d   Galang, Giselle ^a,b,d   Evangelista, Melissa ^a,d   Deo, Rahul C ^b,c,d   Srivastava, Deepak ^a,b  

^a GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Hcn4 protein, mouse; High throughput RNA sequencing; Isl1 protein, mouse; Laser capture microdissection; Sinoatrial node; Transcription factors

Indexed keywords

BIOLOGICAL FACTOR; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR ISLET 1; TRANSCRIPTOME; UNCLASSIFIED DRUG; INSULIN GENE ENHANCER BINDING PROTEIN ISL-1; LIM HOMEODOMAIN PROTEIN; MESSENGER RNA;

ADULT; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION; GENE REGULATORY NETWORK; LASER CAPTURE MICRODISSECTION; MOUSE; NONHUMAN; PACEMAKER NERVE CELL; PRIORITY JOURNAL; RNA SEQUENCE; SINUS NODE; ANIMAL; BIOSYNTHESIS; CYTOLOGY; DEFICIENCY; EMBRYOLOGY; FEMALE; FETUS HEART; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; HEART ATRIUM; HEART CONTRACTION; HEART MUSCLE CELL; HIGH THROUGHPUT SEQUENCING; IMAGE SUBTRACTION; ISOLATION AND PURIFICATION; MALE; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; REPORTER GENE;

ANIMALS; FEMALE; FETAL HEART; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE REGULATORY NETWORKS; GENES, REPORTER; HEART ATRIA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; LASER CAPTURE MICRODISSECTION; LIM-HOMEODOMAIN PROTEINS; MALE; MICE; MOLECULAR SEQUENCE DATA; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; RNA, MESSENGER; SINOATRIAL NODE; SUBTRACTION TECHNIQUE; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSCRIPTOME;

EID: 84924544044     PISSN: 00097330     EISSN: 15244571     Source Type: Journal    
DOI: 10.1161/CIRCRESAHA.116.305913     Document Type: Article

References (17)

1. Qian L, Srivastava D. Direct cardiac reprogramming: from developmental biology to cardiac regeneration. Circ Res. 2013;113:915-921. doi: 10.1161/CIRCRESAHA.112.300625.
2. Takahashi K. Cellular reprogramming. Cold Spring Harb Perspect Biol. 2014;6.
3. Wiese C, Grieskamp T, Airik R, Mommersteeg MT, Gardiwal A, de Gierde Vries C, Schuster-Gossler K, Moorman AF, Kispert A, Christoffels VM. Formation of the sinus node head and differentiation of sinus node myocardium are independently regulated by Tbx18 and Tbx3. Circ Res. 2009;104:388-397. doi: 10.1161/CIRCRESAHA.108.187062.
4. Hoogaars WM, Engel A, Brons JF, Verkerk AO, de Lange FJ, Wong LY, Bakker ML, Clout DE, Wakker V, Barnett P, Ravesloot JH, Moorman AF, Verheijck EE, Christoffels VM. Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria. Genes Dev. 2007;21:1098-1112. doi: 10.1101/gad.416007.
5. Blaschke RJ, Hahurij ND, Kuijper S, et al. Targeted mutation reveals essential functions of the homeodomain transcription factor Shox2 in sinoatrial and pacemaking development. Circulation. 2007;115:1830-1838. doi: 10.1161/CIRCULATIONAHA.106.637819.
6. Tessadori F, van Weerd JH, Burkhard SB, Verkerk AO, de Pater E, Boukens BJ, Vink A, Christoffels VM, Bakkers J. Identification and functional characterization of cardiac pacemaker cells in zebrafish. PLoS One. 2012;7:e47644. doi: 10.1371/journal.pone.0047644.
7. Weinberger F, Mehrkens D, Friedrich FW, Stubbendorff M, Hua X, Müller JC, Schrepfer S, Evans SM, Carrier L, Eschenhagen T. Localization of Islet-1-positive cells in the healthy and infarcted adult murine heart. Circ Res. 2012;110:1303-1310. doi: 10.1161/CIRCRESAHA.111.259630.
8. Bakker ML, Boink GJ, Boukens BJ, Verkerk AO, van den Boogaard M, den Haan AD, Hoogaars WM, Buermans HP, de Bakker JM, Seppen J, Tan HL, Moorman AF, 't Hoen PA, Christoffels VM. T-box transcription factor TBX3 reprogrammes mature cardiac myocytes into pacemaker-like cells. Cardiovasc Res. 2012;94:439-449. doi: 10.1093/cvr/cvs120.
9. Kapoor N, Liang W, Marbán E, Cho HC. Direct conversion of quiescent cardiomyocytes to pacemaker cells by expression of Tbx18. Nat Biotechnol. 2013;31:54-62. doi: 10.1038/nbt.2465.
10. Hu YF, Dawkins JF, Cho HC, Marban E, Cingolani E. Biological pacemaker created by minimally invasive somatic reprogramming in pigs with complete heart block. Sci Transl Med. 2014;6:245ra294.
11. Nam YJ, Lubczyk C, Bhakta M, Zang T, Fernandez-Perez A, McAnally J, Bassel-Duby R, Olson EN, Munshi NV. Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors. Development. 2014;141:4267-4278. doi: 10.1242/dev.114025.
12. Yasui K, Liu W, Opthof T, Kada K, Lee JK, Kamiya K, Kodama I. I(f) current and spontaneous activity in mouse embryonic ventricular myocytes. Circ Res. 2001;88:536-542.
13. 2+ channel in atrioventricular node automaticity. J Mol Cell Cardiol. 2011;50:194-202. doi: 10.1016/j.yjmcc.2010.10.002.
14. Liang X, Wang G, Lin L, Lowe J, Zhang Q, Bu L, Chen Y, Chen J, Sun Y, Evans SM. HCN4 dynamically marks the first heart field and conduction system precursors. Circ Res. 2013;113:399-407. doi: 10.1161/CIRCRESAHA.113.301588.
15. Morris SA, Cahan P, Li H, Zhao AM, San Roman AK, Shivdasani RA, Collins JJ, Daley GQ. Dissecting engineered cell types and enhancing cell fate conversion via CellNet. Cell. 2014;158:889-902. doi: 10.1016/j.cell.2014.07.021.
16. Cai CL, Liang X, Shi Y, Chu PH, Pfaff SL, Chen J, Evans S. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart. Dev Cell. 2003;5:877-889.
17. Hoffmann S, Berger IM, Glaser A, Bacon C, Li L, Gretz N, Steinbeisser H, Rottbauer W, Just S, Rappold G. Islet1 is a direct transcriptional target of the homeodomain transcription factor Shox2 and rescues the Shox2-mediated bradycardia. Basic Res Cardiol. 2013;108:339. doi: 10.1007/s00395-013-0339-z.