High-level expression of a novel α-galactosidase gene from Rhizomucor miehei in Pichia pastoris and characterization of the recombinant enyzme

Protein Expression and Purification

Volumn 110, Issue , 2015, Pages 107-114

  Chen, Zhou ^a   Yan, Qiaojuan ^a   Jiang, Zhengqiang ^a   Liu, Yu ^a   Li, Yuchen ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Expression; Pichia pastoris; Rhizomucor miehei; Galactosidase

Indexed keywords

FUNGI; PICHIA PASTORIS; RHIZOMUCOR MIEHEI;

ALPHA GALACTOSIDASE; FUNGAL PROTEIN; OLIGOSACCHARIDE; RAFFINOSE; RECOMBINANT PROTEIN; STACHYOSE;

AMINO ACID SEQUENCE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; FERMENTATION; GENE EXPRESSION; GENETICS; INTRON; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PICHIA; PLASMID; PROTEIN MULTIMERIZATION; RHIZOMUCOR; TEMPERATURE;

ALPHA-GALACTOSIDASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; FERMENTATION; FUNGAL PROTEINS; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; INTRONS; KINETICS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; OPEN READING FRAMES; PICHIA; PLASMIDS; PROTEIN MULTIMERIZATION; RAFFINOSE; RECOMBINANT PROTEINS; RHIZOMUCOR; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84924247720     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2015.02.015     Document Type: Article

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