High-level expression of a specific β-1,3-1,4-glucanase from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 88, Issue 2, 2010, Pages 509-518

  Hua, Chengwei ^a   Yan, Qiaojuan ^a   Jiang, Zhengqiang ^a   Li, Yinan ^a   Katrolia, Priti ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

1,3 1,4 Glucanase; Cloning; Efficient expression; Lichenase; Paecilomyces thermophila; Pichia pastoris

Indexed keywords

EFFICIENT EXPRESSION; GLUCANASE; LICHENASE; PAECILOMYCES; PICHIA PASTORIS;

AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CLONING; ENZYMES; GEL PERMEATION CHROMATOGRAPHY; GELS; GENE EXPRESSION; ION CHROMATOGRAPHY; ION EXCHANGE; ORGANIC ACIDS; PURIFICATION;

ENZYME ACTIVITY;

AMINO ACID; ENDO 1,3(4) BETA GLUCANASE;

AMINO ACID; CHROMATOGRAPHY; ENZYME; GENE EXPRESSION; ION EXCHANGE; PH; THERMOPHILY; YEAST;

AMINO ACID SEQUENCE; ARTICLE; BIOREACTOR; CLONING; CONTROLLED STUDY; ENZYME PURIFICATION; ENZYME SPECIFICITY; FUNGAL GENE; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; GENE SEQUENCE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NEOSARTORYA; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PAECILOMYCES; PAECILOMYCES THERMOPHILA; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECRETION; ROOM TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENDO-1,3(4)-BETA-GLUCANASE; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PAECILOMYCES; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

FUNGI; NEOSARTORYA FISCHERI NRRL 181; PAECILOMYCES SP.; PICHIA PASTORIS;

EID: 77956230634     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2759-0     Document Type: Article

References (41)

1. ML Bang I Villadsen T Sandal 1999 Cloning and characterization of an endo-β-1, 3(4)glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938 Appl Microbiol Biotechnol 51 2 215 222 10.1007/s002530051384 1:CAS:528:DyaK1MXitVSrurY%3D
2. MW Bauer LE Driskill W Callen MA Snead EJ Mathur RM Kelly 1999 An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes β-1, 4 bonds in mixed-linkage (1→3), (1→4)-β-D- glucans and cellulose J Bacteriol 181 1 284 290 1:CAS:528:DyaK1MXitFWmtA%3D%3D
3. KM Bischoff AP Rooney XL Li S Liu SR Hughes 2006 Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis Biotechnol Lett 28 21 1761 1765 10.1007/s10529-006-9153-0 1:CAS:528:DC%2BD28XhtVSitLnP
4. KR Celestino RB Cunha CR Felix 2006 Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry BMC Biochem 7 23 10.1186/1471-2091-7-23
5. JL Cereghin JM Cregg 2000 Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol Rev 24 45 66 10.1111/j.1574-6976.2000.tb00532.x
6. H Chen XL Li LG Ljungdahl 1997 Sequencing of a 1, 3-1, 4-β-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin J Bacteriol 179 6028 6034 1:CAS:528: DyaK2sXmsFCis7s%3D
7. R Daly MT Hearn 2005 Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production J Mol Recognit 18 2 119 138 10.1002/jmr.687 1:CAS:528:DC%2BD2MXitFOns7Y%3D
8. MS Ekinci SI McCrae HJ Flint 1997 Isolation and overexpression of a gene encoding an extracellular β-(1, 3-1, 4)-glucanase from Streptococcus bovis JB1 Appl Environ Microbiol 63 3752 3756 1:CAS:528:DyaK2sXms1Sgsrk%3D
9. LL Fu ZR Xu JB Shuai CX Hu W Dai WF Li 2008 High-level secretion of a chimeric thermostable lichenase from Bacillus subtilis by screening of site-mutated signal peptides with structural alterations Curr Microbiol 56 3 287 292 10.1007/s00284-007-9077-5 1:CAS:528:DC%2BD1cXhs1Wnt7k%3D
10. OJ Gaiser K Piotukh MN Ponnuswamy A Planas R Borriss U Heinemann 2006 Structural basis for the substrate specificity of a Bacillus 1, 3-1, 4-β-glucanase J Mol Biol 357 1211 1225 10.1016/j.jmb.2006.01.014 1:CAS:528:DC%2BD28Xit1Ogt7w%3D
11. JM Görlach E van der Knaap JD Walton 1998 Cloning and targeted disruption of MLG1, a gene encoding two of three extracellular mixed-linked glucanases of Cochliobolus carbonum Appl Environ Microbiol 64 385 391
12. SG Grishutin AV Gusakov EI Dzedzyulya AP Sinitsyn 2006 A lichenase-like family 12 endo-(1, 4)-β-glucanase from Aspergillus japonicus: study of the substrate specificity and mode of action on β-glucans in comparison with other glycoside hydrolases Carbohydr Res 341 218 229 10.1016/j.carres.2005.11. 011 1:CAS:528:DC%2BD28Xhs12lsA%3D%3D
13. H Huang P Yang H Luo H Tang N Shao T Yuan Y Wang Y Bai B Yao 2008 High-level expression of a truncated 1, 3-1, 4-β-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation Appl Microbiol Biotechnol 78 1 95 103 10.1007/s00253-007-1290-4 1:CAS:528:DC%2BD1cXot1KmtQ%3D%3D
14. R Kawai K Igarashi M Yoshida M Kitaoka M Samejima 2006 Hydrolysis of β-1, 3/1, 6-glucan by glycoside hydrolase family 16 endo-1, 3(4)-β-glucanase from the basidiomycete Phanerochaete chrysosporium Appl Microbiol Biotechnol 71 6 898 906 10.1007/s00253-005-0214-4 1:CAS:528: DC%2BD28Xns1Smsrg%3D
15. E Kitamura Y Kamei 2006 Molecular cloning of the gene encoding β-1, 3(4)-glucanase A from a marine bacterium, Pseudomonas sp. PE2, an essential enzyme for the degradation of Pythium porphyrae cell walls Appl Microbiol Biotechnol 71 5 630 637 10.1007/s00253-005-0200-x 1:CAS:528:DC%2BD28Xnt1aju74%3D
16. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 10.1038/227680a0 1:CAS:528:DC%2BD3MXlsFags7s%3D
17. OH Lowry NJ Rosebrough AL Farr RJ Randall 1951 Protein measurement with the folin phenol reagent J Biol Chem 193 265 275 1:CAS:528:DyaG38XhsVyrsw%3D%3D
18. H Luo J Yang P Yang J Li H Huang P Shi Y Bai Y Wang Y Fan B Yao 2010 Gene cloning and expression of a new acidic family 7 endo-β-1, 3-1, 4-glucanase from the acidophilic fungus Bispora sp. MEY-1 Appl Microbiol Biotechnol 85 4 1015 1023 10.1007/s00253-009-2119-0 1:CAS:528:DC%2BC3cXjslWkuw%3D%3D
19. M Mansur C Cabello L Hernández J País L Varas J Valdés Y Terrero A Hidalgo L Plana V Besada L García E Lamazares L Castellanos E Martínez 2005 Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris Biotechnol Lett 27 339 345 10.1007/s10529-005-1007-7 1:CAS:528:DC%2BD2MXjtlKmur8%3D
20. BV McCleary 1988 Purification of 1-3, 1-4-β-D-glucan from barley flour Meth Enzymol 160 511 514 10.1016/0076-6879(88)60160-1 1:CAS:528: DyaL1cXls12iurc%3D
21. NP Mchunu S Singh K Permaul 2009 Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli J Biotechnol 141 1-2 26 30 10.1016/j.jbiotec.2009.02.021 1:CAS:528: DC%2BD1MXkslOrs7c%3D
22. GL Miller 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal Chem 31 426 428 10.1021/ac60147a030 1:CAS:528: DyaG1MXmtFKiuw%3D%3D
23. PG Murray A Grassick CD Laffey MM Cuffe T Higgins AV Savage A Planas MG Tuohy 2001 Isolation and characterization of a thermostable endo-β- glucanase active on 1, 3-1, 4-β-D-glucans from the aerobic fungus Talaromyces emersonii CBS 814.70 Enzyme Microb Technol 29 90 98 10.1016/S0141-0229(01)00354-4 1:CAS:528:DC%2BD3MXks1OlsLg%3D
24. A Planas 2000 Bacterial 1, 3-1, 4-β-glucanases: structure, function and protein engineering Biochim Biophys Acta 1543 361 382 1:CAS:528: DC%2BD3MXitl2ksg%3D%3D
25. TM Rose JG Henikoff S Henikoff 2003 CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design Nucl Acids Res 31 13 3763 3766 10.1093/nar/gkg524 1:CAS:528:DC%2BD3sXltVWjsb4%3D
26. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
27. S Schimming WH Schwarz 1991 Properties of a thermoactive β-1, 3-1, 4-glucanase (Lichenase) from Clostridium thermocellum expressed in Escherichia coli Biochem Biophys Res Commun 177 447 452 10.1016/0006-291X(91)92004-4 1:CAS:528:DyaK3MXmt1Klur0%3D
28. CA Scorer JJ Clare WR McCombie MA Romanos K Sreekrishna 1994 Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression Biotechnol NY 12 2 181 184 10.1038/nbt0294-181 1:CAS:528:DyaK2cXmt1eisro%3D
29. MP Sue LF Mariana M Brian MH Linda 2005 Heterologous protein production using the Pichia pastoris expression system Yeast 22 249 270 10.1002/yea.1208
30. RM Teather JD Erfle 1990 DNA sequence of a Fibrobacter succinogenes mixed-linkage 3-glucanase (1, 3-1, 4-β-D-glucan 4-glucanohydrolase) gene J Bacteriol 172 3837 3841 1:CAS:528:DyaK3cXmtVGlsbg%3D
31. D Teng Y Fan Y Yang Z Tian J Luo J Wang 2007 Codon optimization of Bacillus licheniformis β-1, 3-1, 4-glucanase gene and its expression in Pichia pastoris Appl Microbiol Biotechnol 74 1074 1083 10.1007/s00253-006-0765-z 1:CAS:528:DC%2BD2sXjtVSqur4%3D
32. A Vassileva DA Chugh S Swaminathan N Khanna 2001 Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris Protein Expr Purif 21 71 80 10.1006/prep.2000.1335 1:CAS:528:DC%2BD3MXmsFCisA%3D%3D
33. J Vasur R Kawai E Andersson K Igarashi M Sandgren M Samejima J Ståhlberg 2009 X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis FEBS J 276 3858 3869 10.1111/j.1742-4658.2009.07099.x 1:CAS:528: DC%2BD1MXovV2ltrw%3D
34. JL Wang H Ruan HF Zhang Q Zhang HB Zhang GQ He SR Shen 2007 Characterization of a thermostable and acidic-tolerable β-glucanase from aerobic fungi Trichoderma koningii ZJU-T J Food Sci 72 C452 C456 10.1111/j.1750-3841.2007.00549.x 1:CAS:528:DC%2BD2sXhsValtb3J
35. TN Wen JL Chen SH Lee NS Yang LF Shyur 2005 A truncated Fibrobacter succinogenes 1, 3-1, 4-β-D-glucanase with improved enzymatic activity and thermotolerance Biochemistry 44 9197 9205 10.1021/bi0500630 1:CAS:528: DC%2BD2MXks1Sntrc%3D
36. A-S Xiong Q-H Yao R-H Peng P-L Han Z-M Cheng Y Li 2005 High level expression of a recombinant acid phytase gene in Pichia pastoris J Appl Microbiol 98 2 418 428 10.1111/j.1365-2672.2004.02476.x 1:CAS:528: DC%2BD2MXhvVGjsL4%3D
37. M Yaish A Doxey B McConkey B Moffatt M Griffith 2006 Coldactive winter rye glucanases with ice-binding capacity Plant Physiol 141 1459 1472 10.1104/pp.106.081935 1:CAS:528:DC%2BD28XosVKit7Y%3D
38. SQ Yang QJ Yan ZQ Jiang LT Li HM Tian YZ Wang 2006 High-level of xylanase production by the thermophilic Paecilomyces thermophila J18 on wheat straw in solid-state fermentation Bioresour Technol 97 1794 1800 10.1016/j.biortech.2005. 09.007 1:CAS:528:DC%2BD28XmsVyjsLo%3D
39. P Yang P Shi Y Wang Y Bai K Meng H Luo T Yuan B Yao 2007 Cloning and overexpression of a Paenibacillus β-glucanase in Pichia pastoris: purification and characterization of the recombinant enzyme J Microbiol Biotechnol 17 58 66
40. SQ Yang QJ Yan ZQ Jiang GS Fan L Wang 2008 Biochemical characterization of a thermostable novel β-1, 3-1, 4-glucanase (lichenase) from Paecilomyces thermophila J Agric Food Chem 56 13 5345 5351 10.1021/jf800303b 1:CAS:528:DC%2BD1cXntVygu78%3D
41. K Yaoi Y Mitsuishi 2004 Purification, characterization, cDNA cloning, and expression of a xyloglucan endoglucanase from Geotrichum sp. M128 FEBS Lett 560 1-3 45 50 10.1016/S0014-5793(04)00068-7 1:CAS:528:DC%2BD2cXhsVKhur4%3D